HE Shuling,MA Lingfa,YANG Jingjun,et al.Effect of Exogenous SA on Physiological Conditions and Membrane Damage of Rhodiola kirilowii Seedling Under Stress of Low Temperature[J].Northern Horticulture,2015,39(17):61-65.[doi:10.11937/bfyy.201517016]
外源水杨酸对低温胁迫下狭叶红景天幼苗生理及膜伤害的影响
- Title:
- Effect of Exogenous SA on Physiological Conditions and Membrane Damage of Rhodiola kirilowii Seedling Under Stress of Low Temperature
- Keywords:
- Rhodiola kirilowii; stress of low temperature; exogenous substance; membrane damage; physiological condition
- 文献标志码:
- A
- 摘要:
- 以狭叶红景天幼苗为试材，在0℃低温条件下，研究外源SA对狭叶红景天幼苗过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、过氧化物酶(POD)3种抗氧化酶活性、MDA含量以及膜伤害的影响，探讨外源SA对低温胁迫下红景天幼苗生理及膜伤害的影响。结果表明：在0℃低温胁迫条件下，不同浓度的外源SA总体上均使红景天幼苗叶片CAT、POD、SOD活性不同程度升高，使叶片MDA含量不同程度降低；使叶绿体膜Ca2+-ATP活性、Mg2+-ATP活性不同程度增强。在低温胁迫下，外源SA通过提高抗氧化酶活性、抗氧化物质含量及渗透调节物质含量，减少了膜脂过氧化产物MDA的产生，叶绿体膜Ca2+-ATP活性、Mg2+-ATP活性的增强，减轻细胞膜的损伤，提高了狭叶红景天幼苗耐低温胁迫的能力；提高狭叶红景天耐寒力的外源物质SA适宜浓度为0.1~0.3 mg/L，SA的最适浓度为0.3 mg/L。
- Abstract:
- Taking Rhodiola kirilowii seedlings as test material，under the low temperature at 0℃，the effect of exogenous SA on activities of three antioxidant enzymes including CAT，SOD and POD，MDA content and membrane damage of Rhodiola kirilowii were studied.The results showed that under stress of low temperature at 0℃，exogenous SA at different concentrations could elevate activities of CAT，POD and SOD in leaves of Rhodiola kirilowii seedling to some extents，decreased MDA content of leaves to some levels and enhanced activities of Ca2+-ATP enzyme and Mg2+-ATP enzyme in the chloroplast membrane at variable degrees.Under the stress of low temperature，exogenous SA would decrease production of MDA which was a kind of membrane lipid peroxidation product，enhance activities of Ca2+-ATP enzyme and Mg2+-ATP enzyme in the chloroplast membrane and ease damage to the cell membrane by enhancing activity of antioxidant enzyme，content of antioxidants and osmolytes，which could improve capabilities of Rhodiola kirilowii seedling to resist low temperature，where the appropriate concentrations of exogenous SA ranged from 0.1 mg/L to 0.3 mg/L and the most appropriate concentration of SA was 0.3 mg/L.
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备注/Memo
第一作者简介：何淑玲（1975-），女，博士，副教授，研究方向为药用植物资源与利用。E-mail：heshuling2@163.com.基金项目：国家星火计划资助项目（2010GA860031）；甘肃民族师范学院院长基金资助项目（14-16，14-17）。