YUE Wei,YANG Wenting,WU Gege,et al.Optimization and Comparison of Extraction Technology of Flavonoids From Leaves of Indocalamus iatifolius[J].Northern Horticulture,2015,39(16):131-134.[doi:10.11937/bfyy.201516031]
箬叶总黄酮提取工艺优化及比较
- Title:
- Optimization and Comparison of Extraction Technology of Flavonoids From Leaves of Indocalamus iatifolius
- Keywords:
- flavonoids from leaves of indocalamus; ethanol refluxing method; ethanol ultrasonic method
- 文献标志码:
- A
- 摘要:
- 以阔叶箬竹叶为试材，采用单因素和正交实验，研究了回流法和超声法中乙醇浓度、料液比、提取时间等对箬叶总黄酮得率的影响，并优化了提取工艺。结果表明：回流法最佳工艺是提取溶剂85%乙醇溶液、料液比1∶20 g/mL、提取回流时间50 min；超声法的最佳提取工艺条件是提取溶剂80%乙醇溶液、料液比1∶30 g/mL、提取时间35 min；优化条件下，回流法的箬叶总黄酮的提取率达到2.66%；超声法的提取率为1.59%，回流法比超声法更适合于箬叶总黄酮的提取。
- Abstract:
- Taking leaves of Indocalamus latifolius as experimental material，the effect of the ethanol concentration,solid-liquid ratio,extraction time on the yield of total flavonoids were studied by the single factor experiments and orthogonal experiments,and the extraction technology was optimized.The results showed that the optimized extraction process of ethanol refluxing method,the extraction solvent was 85% ethanol，material/liquid ratio was 1∶20 g/mL，extracting time was 50 min.The optimized extraction process of ethanol ultrasonic method：the extraction solvent was 85% ethanol，material/liquid ratio was 1∶30 g/mL，extracting time was 35 min.Under the optimum process conditions,the yield of total flavonoids from leaves of indocalamus by ethanol refluxing method was 2.66%，the yield by ethanol ultrasonic method was 1.59%，the ethanol refluxing method was more suitable for the extraction of flavonoids.
参考文献/References:
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备注/Memo
第一作者简介：乐薇（1979-），女，硕士，副教授，现主要从事生物分析等研究工作。E-mail：yuewei11@126.com. 基金项目：湖北省自然科学基金资助项目（2013C111）。