QU Yongqing,WANG Ran,MA Chunhui,et al.Genetic Diversity Analysis of Pear Genus in Shanxi Province by SSR Marker[J].Northern Horticulture,2015,39(16):11-17.[doi:10.11937/bfyy.201516003]
山西梨属植物资源的SSR遗传多样性分析
- Title:
- Genetic Diversity Analysis of Pear Genus in Shanxi Province by SSR Marker
- Keywords:
- pear; wild resource; SSR; genetic diversity
- 文献标志码:
- A
- 摘要:
- 以50份野生梨属植物资源为试材，应用SSR分子标记技术对其进行了遗传多样性分析，以探索山西省梨属植物资源的遗传多样性。结果表明：筛选的40对SSR引物扩增得到236个等位基因位点，平均每对引物扩增得到5.90个等位点。不同样品间遗传多样性指数为0.276 2～0.431 9，检测位点杂合度为0.335 0～0.775 0，多态信息含量变化范围在0.236 8～0.431 2，表明4个遗传多样性参数均表现出较高的遗传多样性。聚类分析结果显示，50份样品相似系数的变化范围在0.444 9～0.944 9，样品在相似系数0.57处被分为四大类群，类群I为豆梨类，类群II、III、IV为杜梨类；其中，类群II、III集中了大部分白家山和绛县地区的样品，类群IV的样品全部来自太谷，表明山西梨属植物资源的聚类结果与样品的地理分布基本吻合；发现大果、绿皮杜梨野生资源。
- Abstract:
- To explore the genetic diversity of wild pear genus in Shanxi Province，the genetic diversity of 50 wild simples of Pyrus were analyzed using SSR molecular marker technique in this study，the genetic diversity of 50 wild pear genus were analyzed by SSR molecular marker technique.The results showed that，236 alleles were amplified by 40 pairs of SSR primers and 5.90 alleles were amplified by per primers.The genetic diversity analysis showed that the genetic diversity indexes varied from 0.276 2 to 0.431 9，the expected heterozygosity were 0.335 0 to 0.775 0，and the polymorphism information content ranged from 0.236 8 to 0.431 2.The diversity of the 50 wild pear resources were showed high by the four parameters.The clustering analysis results showed that the similarity coefficient of the 50 cultivars ranged from 0.444 9 to 0.839 0，all the cultivars were divided into four groups at the similarity coefficient of 0.57，the cultivars of group I belonged to Pyrus calleryana，the cultivars of group II，III and IV belonged to Pyrus betulaefolia.The most cultivars of Baijia mountain and Jiang county belonged to the group II and group III，however，all the cultivars of Taigu county belonged to group IV，which accorded with the geographic distribution；we also found new Pyrus betulaefolia with big fruit and green skin.
参考文献/References:
[1]李秀根,张绍铃.世界梨产业现状与发展趋势分析[J].烟台果树,2007(1):12-17. [2]李树玲，曹玉芬,黄礼森,等.大鸭梨与不同倍性梨品种杂交后代染色体数目鉴定[J].天津农学院学报,1998,5(4):1-7. [3]曲柏宏,严花淑,陈艳秋，等.同工酶分析在梨品种分类中的应用[J].延边大学农学学报,2003,25(1):86-91. [4]BAO L，CHEN K，ZHANG D，et al.An assessment of genetic variability and relationships within Asian pears based on AFLP (Amplified Fragment Length Polymorphism) markers[J].Sci Hortic,2008,116(4):374-380. [5]BAO L，CHEN K，ZHANG D，et al.Genetic diversity and similarity of pear(Pyrus L.) cultivars native to east Asia revealed by SSR (Simple Sequence Repeat) markers[J].Genet Resour Crop Evol,2007,54(5):959-971. [6]张东.中国红皮砂梨遗传多样性及亲缘关系的SSR和AFLP分析[D].杭州:浙江大学,2006. [7]曹玉芬,刘凤之,高源,等.梨栽培品种SSR鉴定及遗传多样性[J].园艺学报,2007,34(2):305-310. [8]杨军.梨野生资源及杂交F-1代的遗传多样性研究[D].南京:南京农业大学,2012. [9]管晓庆,王奎玲,刘庆华,等.RAPD技术在我国观赏植物中的应用[J].北方园艺,2007(4):75-77. [10]王永清,付燕,杨岑,等.枇杷属植物遗传多样性的ISSR分析[J].林业科学,2010,46(4):49-57. [11]许云亭,吴文墙,谢俊,等.111份大暑种质资源遗传多样性AFLP分析[J].植物遗传资源学报,2014,15(2):299-304. [12]沈玉英,滕元文,田边贤二.部分中国沙梨和日本梨的RAPD分析[J].园艺学报,2006,33(3):621-624. [13]朱立武,王艳芳,贾兵,等.安徽砀山酥梨自然保护区梨种质资源AFLP分析[J].果树学报,2009,26(2):145-150. [14]蔡丹英,范太伟,滕元文,等.甘肃中部梨种质资源的AFLP分析[J].果树学报,2008,25(3):298-304. [15]路娟,吴俊,张绍铃,等.基于苹果EST-SSR的梨种质资源遗传多样性分析[J].西北植物学报,2010,30(4):645-651. [16]YAMAMOTO T，KIMURA T，SAITO T,et al.Genetic linkage maps of Japanese and European pears aligned to the apple consensus map[J].Acta Hort,2004,663:51-55. [17]HAN Y，ZHENG D,VIMOLMANGKANG S，et al.Integration of physical and genetic maps in apple confirms whole-genome and segmental duplications in the apple genome[J].Exp Bot,2011,62(14):5117-5130. [18]许靖诗,乌云塔娜,叶生晶,等.梨种质资源SSR引物的筛选与评价[J].中南林业科技大学学报,2012,32(7):80-85. [19]LIU K，MUSE S V.Power marker：an integrated analysis environment for genetic marker data[J].Bioinformatics (Oxford England),2005,21(9):2121-2129. [20]PEAKALL R，SMOUSE P E.GENALEX 6：genetic analysis in excel.Population genetic software for teaching and research[J].Mol Ecol Notes,2006,6(1):288-295. [21]TAKEZAK I N，NEI M.Genetic distances and reconstruction of phylogenetic trees from microsatellite DNA[J].Genetics,1996,144(1):389-399. [22]宗宇,孙萍,牛庆丰,等.中国北方野生杜梨分布现状及其形态多样性评价[J].果树学报,2013,30(6):918-923. [23]杨庆文,秦文斌,张万霞,等.中国农业野生植物原生境保护实践与未来研究方向[J].植物遗传资源学报,2013,14(1):1-7.
相似文献/References:
[1]侯玉华,孙 正,张玉星.河北省果品梨流通体系的现状、问题及对策[J].北方园艺,2013,37(22):201.
HOU Yu-hua,SUN Zheng,ZHANG Yu-xing.Study on the Current Situation,Problems and Countermeasures of the Pear Circulation in Hebei Province[J].Northern Horticulture,2013,37(16):201.
[2]冯娟娟,刘军弟,霍学喜.世界主要落叶水果主产国生产与贸易预测分析[J].北方园艺,2014,38(04):195.
[3]王尚堃,杜红阳.“晚秋黄”梨省力化高效丰产栽培技术[J].北方园艺,2014,38(06):59.
[J].Northern Horticulture,2014,38(16):59.
[4]张晓峰,韩翔,张建光.不同土壤水分含量对黄冠梨叶片光合效率和抗氧化特性的影响[J].北方园艺,2013,37(10):7.
ZHANG Xiao-feng,HAN Xiang,ZHANG Jian-guang.Effect of Different Soil Moisture Contents on Net Photosynthetic Rate and Antioxidant Abilities in Huangguan Pear Leaves[J].Northern Horticulture,2013,37(16):7.
[5]贾春霞,张小珍,马春晖.梨园春季晚霜冻害发生与预防技术[J].北方园艺,2013,37(11):197.
JIA Chun-xia,ZHANG Xiao-zhen,MA Chun-hui.Causes and Preventing Techniques of Frost Injury in the Pear Orchard[J].Northern Horticulture,2013,37(16):197.
[6]刘珠琴,舒巧云,蒋国强,等.梨人工辅助授粉时间、花朵序位及方法研究[J].北方园艺,2012,36(01):35.
LIU Zhu-qin,SHU Qiao-yun,JIANG Guo-qiang,et al.Study on the Pear Assisted Pollination Time, Flowers Ordinal and Method[J].Northern Horticulture,2012,36(16):35.
[7]李金龙,马光恕.不同储藏方式对梨果实硬度及PPO活性的影响[J].北方园艺,2013,37(09):164.
LI Jin-long,MA Guang-shu.Effect of Different Storage Methods on the Fruit Firmness and PPO Activity of Pear[J].Northern Horticulture,2013,37(16):164.
[8]董晓勤,周 鹏,仇宗浩,等.两个品种绿皮梨及其红色芽变品种光合生理特性的比较[J].北方园艺,2013,37(20):1.
DONG Xiao-qin,ZHOU Peng,QIU Zong-hao,et al.Comparison of Photosynthetic Physiological Characteristics of Two Varieties of Green Pears and Their Red Mutations Varieties[J].Northern Horticulture,2013,37(16):1.
[9]徐凌飞,李致慧,贾东峰,等.梨矮化砧木云南榅桲离体快繁研究[J].北方园艺,2012,36(05):113.
XU Ling-fei,LI Zhi-hui,JIA Dong-feng,et al.Study on in vitro Propagation of Yunnan Quince as a Dwarf Rootstock of Pear[J].Northern Horticulture,2012,36(16):113.
[10]王俊英,李富军.1-甲基环丙烯对“丰水梨”冷藏品质的影响[J].北方园艺,2012,36(18):165.
Influence of -MCP on Hosui Pear Storage Quality.[J].Northern Horticulture,2012,36(16):165.
备注/Memo
第一作者简介：曲永青（1987-），女，硕士研究生，研究方向为果树栽培生理与分子生物学。E-mail:nongxueyuan123ko@163.com.责任作者：马春晖（1966-），男，博士，副教授，研究方向为果树栽培生理。E-mail：machunhui2000@163.com.基金项目：山东省自然科学基金资助项目(Y2007D64)；国家现代农业(梨)产业技术体系建设专项资助项目(Nycytx-29-06)。