[1]段雪甜,陈雨薇,王芳,等.连瓣兰组织培养及菌根化研究[J].北方园艺,2015,39(15):111-113.[doi:10.11937/bfyy.201515029]
　DUAN Xuetian,CHEN Yuwei,WANG Fang,et al.Study on Tissue Culture and Mycorrhizal Fungi of Cymbidium lianpan[J].Northern Horticulture,2015,39(15):111-113.[doi:10.11937/bfyy.201515029]
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连瓣兰组织培养及菌根化研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第39卷 期数: 2015年15 页码: 111-113 栏目: 出版日期: 2015-08-15
Title:
Study on Tissue Culture and Mycorrhizal Fungi of Cymbidium lianpan
作者:
段雪甜1; 2; 陈雨薇1; 2; 王芳1; 李婷婷2; 伍建榕1; 2
（1.西南林业大学 国家林业局西南地区生物多样性保育重点实验室，云南 昆明 650224； 2.西南林业大学 林学院，云南省高校森林灾害预警控制重点实验室，云南 昆明 650224）
Author(s):
DUAN Xuetian1; CHEN Yuwei1; WANG Fang2; LI Tingting1; WU Jianrong1; 2
(1.Key Laboratory of State Forestry Administration for Biodiversity Conservation in Southwest China,Southwest Forestry University,Kunming,Yunnan 650224；2.Key Laboratory of Forest Disaster Warning and Control in Yunnan Higher Education Institutions, College of Forestry, Southwest Forestry University, Kunming,Yunnan 650224)
关键词:
连瓣兰; 组织培养; 菌根真菌; 共生培养; 菌根化
Keywords:
Cymbidium lianpan;  tissue culture; mycorrhizal fungi;  symbiosis; mycorrhizal
DOI:
10.11937/bfyy.201515029
文献标志码:
A
摘要:
以野生连瓣兰和组培连瓣兰为试材，采用内生真菌分离鉴定、内生真菌与连瓣兰组培苗的共生培养以及制作石蜡切片对其菌根结构进行观察等方法，研究菌根真菌对连瓣兰生长的影响，并找出适合连瓣兰生长的菌根真菌。结果表明：从保山野外采集的12株连瓣兰中分离得到的10个菌株中，有3株适合与组培苗进行共生培养，分别是丝核菌属（Rhizoctonia sp.）、毛壳菌属（Chaetomium sp.）、镰刀菌属（Fusarium sp.），其中丝核菌效果最佳，对无菌组培苗生长有良好的促进作用并在无菌组培苗根部形成菌丝结。
Abstract:
Using wild Cymbidium lianpan and Cymbidium seed as test materials, using the method of isolating and identificating endophytic fungus, examining the interaction between aseptic seedlings and endophytic fungus in Cymbidium lianpan and making paraffin section of the mycorrhizal structure observation,to study the effect of mycorrhiza fungi on cymbidium lianpan growth,and find out the suitable mycorrhizal fungi for growth of Cymbidium. The results showed that from Baoshan field collection 12 cymbidium lianpan of 10 strains of isolated Cymbidium, there were three suits with the somaclone symbiotic cultivation, respectively was Rhizoctonia sp., Chaetomium sp., Fusarium sp., among them,the effect of Rhizoctonia sp. was the best, had a good role in promoting growth of sterile somaclone hypha and sterile somaclone in the root formation.

参考文献/References:

 

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备注/Memo

第一作者简介：段雪甜（1992-），女，云南保山人，硕士研究生,研究方向为资源微生物学。E-mail：864230585@qq.com. 责任作者:伍建榕(1963-)，女，福建清流人，博士，教授，现主要从事森林病理学和资源微生物利用等研究工作。E-mail：wujianrong63@aliyun.com. 基金项目：国家自然科学基金资助项目（31360198、31260175）；云南省教育厅科学研究基金资助项目(2013Y123)；云南省高校干热河谷植被恢复创新团队资助项目；云南省重点学科森林保护学资助项目（XKZ200905）；云南省高等学校森林病虫害综合治理教学团队资助项目（20126005）。

更新日期/Last Update: 2015-08-20