HU Bohua,XU Jie,DUAN Dechao,et al.Physiological Response of Celery (Apium graveolens L.) to Cadmium Stress by FTIR Spectroscopy[J].Northern Horticulture,2015,39(15):11-16.[doi:10.11937/bfyy.201515003]
镉胁迫下芹菜生理响应的傅里叶变换红外光谱研究
- Title:
- Physiological Response of Celery (Apium graveolens L.) to Cadmium Stress by FTIR Spectroscopy
- 文献标志码:
- A
- 摘要:
- 镉是一种毒性较强、水溶性大的重金属元素，一旦进入环境就会通过食物链危害人体健康。芹菜作为大众蔬菜，在我国具有较广泛的种植面积，其对镉具有一定的富集能力。现以芹菜为试验对象，采用傅里叶变换红外光谱法（FTIR）研究在不同镉浓度（0、5、10、20、40 mg/L）处理下根、茎和叶化学组分的生理变化。结果表明：根、茎组织在3 410、1 635、1 389、1 065 cm-1处特征峰A/A2 931值基本呈现先降后升最后又降的变化趋势；反映出低浓度Cd2+胁迫对根、茎的生理过程几乎无影响；中浓度的Cd2+可能促进根、茎合成、分泌和运输有机物（碳水化合物、氨基酸、蛋白质、糖类），而高浓度的Cd2+则会抑制有机物的分泌和运输，同时导致根、茎组织细胞壁的果胶甲基化程度升高，根、茎细胞内脂肪族酮类化合物过氧化物产物减少。在叶组织中A/A2 931值则呈现逐步升高的趋势，表明芹菜叶对Cd2+的抗逆性会随着Cd2+浓度的增大而增强。以上结果表明，FTIR法可用于植物对重金属胁迫适应过程的生理学研究。
- Abstract:
- Cd is a highly toxic and highly water soluble element without biological function，which tends to enter the food chain，adversely affecting human health once released into the environment.Celery (Apium graveolens L.) is widely planted as a popular vegetable in our country，and has certain accumulation ability for Cd.In the present study，fourier transform infrared (FTIR) spectrometry was used to investigate the physiological changes in chemical composition of celery exposed to solution containing different Cd2+ concentration (0，5，10，20，40 mg/L).The results showed that after an initial decline，the A/A2 931value of the dominant infrared bands near 3 410 cm-1，1 636 cm-1，1 389 cm-1and 1 065 cm-1 in both roots and stems increased at first and then decreased.This indicated that there was almost no effect on physiological processes of the roots and the stems with low Cd2+ concentration，while at medium Cd2+ concentration the roots and the stems were promoted to coalesce，secret and transport organics (carbohydrates，amino acids，proteins，carbohydrates)，and with high Cd2+ concentration the secretion and transportation of the organics were inhibited.Meanwhile，the methylated pectin in cell wall of the roots and the stems increased，and the peroxide products of ketones in cells of the roots and the stems decreased.The A/A2 931value of the dominant infrared bands in the leaves showed a gradually rising trend，which indicated that the stress resistance of the leaves to Cd2+ increased with the Cd2+ concentration increased.As a result，FTIR technique could be accepted as a way to study the physiological mechanism of plant for adapting to the stress of heavy metals.
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备注/Memo
第一作者简介：胡博华（1992-），男，浙江永康人，本科，研究方向为土壤重金属植物污染化学及农产品安全研究。E-mail：1508138765@qq.com.责任作者：徐劼（1975-），男，博士，讲师，现主要从事土壤重金属植物污染化学及农产品安全等研究工作。E-mail：xujie1688@126.com.基金项目：嘉兴市科技计划资助项目（2012AY1046）；嘉兴学院博士科研启动基金资助项目（70611027）。