KorbanjhonBRAD,WANG Meng,ZHANG Xiaoying.Extraction and Determination of Apple Leaf Total Flavonoids[J].Northern Horticulture,2015,39(14):129-132.[doi:10.11937/bfyy.201514034]
苹果叶总黄酮的提取及其含量测定
- Title:
- Extraction and Determination of Apple Leaf Total Flavonoids
- 文献标志码:
- A
- 摘要:
- 以“红元帅”苹果的干燥叶子为试材，通过单因素试验确定苹果叶总黄酮的最佳提取工艺条件，采用可见分光光度法对苹果叶中总黄酮含量进行测定，以期为苹果叶中总黄酮提取工艺及含量测定提供方法和依据。结果表明：苹果叶总黄酮的最佳提取工艺条件为乙醇作溶剂，乙醇浓度60%，料液比1∶20 g/mL，提取温度为70℃，提取时间为30 min；在选定试验条件下对苹果叶进行含量测定，对芦丁对照品浓度与吸光度进行线性回归，其回归方程为Y=24.821 43X-0.031，R2=0.999 45（n=6），芦丁对照品质量在4～24 μg范围内呈良好的线性关系，在此条件下测定苹果叶中总黄酮平均含量为30.28 mg/g。试验优选出了苹果叶中总黄酮的最佳提取工艺条件及含量测定方法，该方法简单、快速，准确，精密度，稳定性，重现性良好。
- Abstract:
- Taking dry apple leaf of ‘Red delicious’ as test material，the optimized extraction method for the apple leaves was explored by the single factor experiment.The total flavonoids were quantitative determined by visible spectrophotometer,to supply an optimized method and methodological basis for flavonoids extraction from apple leave.The results showed that the optimum extraction conditions of apple leaves flavonoids were diluted in 60% ethanol by the ratio of 1∶20 g/mL，70℃ for 30 minutes.Under the above conditions，the flavonoids concentration in apple leaves was measured and took rutin as standard.The linear regression was good within the range of 4-24 μg of rutin and the equation was Y＝24.821 43X-0.031，R2＝0.999 45 (n=6).The total flavonoid in apple leaves was 30.28 mg/g at average.And had set up an optimized flavonoid extraction and assessment methods for apple leave.This method is simple，rapid，accurate，precision，stable and good repeatable.
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备注/Memo
第一作者简介：库尔班江?巴拉提（1962-），男，维吾尔族，教授，研究方向为中药与天然药物。E-mail：korbanjhon@126.com.基金项目：国家自然科学基金资助项目（21166024）。