[1]刘振东,薛蓓,王泽敏,等.林芝地区两株优良黑木耳菌株的ITS序列验证[J].北方园艺,2015,39(10):98-103.[doi:10.11937/bfyy.201510024]
　LIU Zhen-dong,XUE Bei,WANG Ze-min,et al.Verification of Two Excellent Auricularia auricula Species Based on Internal Transcribed Spacer(ITS) Sequences in Nyingchi Area[J].Northern Horticulture,2015,39(10):98-103.[doi:10.11937/bfyy.201510024]
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林芝地区两株优良黑木耳菌株的ITS序列验证

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第39卷 期数: 2015年10 页码: 98-103 栏目: 出版日期: 2015-05-30
Title:
Verification of Two Excellent Auricularia auricula Species Based on Internal Transcribed Spacer(ITS) Sequences in Nyingchi Area
文章编号:
1001-0009（2015）10-0098-06
作者:
刘振东1; 2; 薛蓓1; 2; 王泽敏1; 2; 杨赟毓1; 2; 张彦龙3
(1.西藏大学 农牧学院 食品科学学院，西藏 林芝 860000；2.西藏野生特色生物资源开发平台，西藏 林芝 860000；3.黑龙江大学 生命科学学院，微生物黑龙江省高校重点实验室，黑龙江 哈尔滨 150000)
Author(s):
LIU Zhen-dong1; 2; XUE Bei1; 2; WANG Ze-min1; 2; YANG Yun-yu1; 2; ZHANG Yan-long3
(1.Department of Food Science，Tibet Agricultural and Animal Husbandry College，Nyingchi,Tibet 860000;2.Platform of Wild Characteristic Biological Resources in Tibet,Nyingchi,Tibet 860000;3.Province Key Laboratory of Microbiology，College of Life Science，Heilongjiang University，Harbin,Heilongjiang 150000)
关键词:
林芝; 黑木耳; ITS; 验证
Keywords:
Nyingchi area; Auricularia auricula; ITS; verification
分类号:
S 646.6
DOI:
10.11937/bfyy.201510024
文献标志码:
A
摘要:
以林芝地区2株优良野生黑木耳菌种“西藏6号”和“西藏7号”为试材，采用ITS序列分析方法对其进行分子生物学鉴定，再用生物软件Clustal X 1.83和MAGA 5.0进行系统发育分析。结果表明：菌株“西藏6号”和“西藏7号”为黑木耳属黑木耳种，通过对二者的碱基序列比对和共同系统发育树的构建，确定二者之间存在一定遗传差异。
Abstract:
Taking two excellent field Auricularia auricula species ‘X6’ and ‘X7’ in Nyingchi area as test material,ITS Sequence Analysis was used to screen,the software Clustal X 1.83 and MAGA 5.0 were used to conduct the phylogeny analysis.The results showed that two excellent field species ‘X6’ and ‘X7’ were Auricularia auricula,comparing the DNA sequence and building a common phylogenetic tree，it was confirmed that there was a certain genetic difference between the two.

参考文献/References:


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备注/Memo

第一作者简介：刘振东（1986-），男，硕士，讲师，研究方向为食品微生物学及天然产物。E-mail：liu304418091@126.com.

责任作者：张彦龙（1966-），男，博士，教授，研究方向为天然产物活性。

更新日期/Last Update: 2015-08-07