[1]孙国珍,高利利,陆文利,等.河北省设施番茄褪绿病毒分子检测和鉴定研究[J].北方园艺,2015,39(09):95-98.[doi:10.11937/bfyy.201509026]
　SUN Guo-zhen,GAO Li-li,LU Wen-li,et al.Molecular Detection and Identification of Tomato chlorosis virus Infecting Greenhouse-grown Tomato Plants in Hebei Province[J].Northern Horticulture,2015,39(09):95-98.[doi:10.11937/bfyy.201509026]
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河北省设施番茄褪绿病毒分子检测和鉴定研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第39卷 期数: 2015年09 页码: 95-98 栏目: 出版日期: 2015-05-15
Title:
Molecular Detection and Identification of Tomato chlorosis virus Infecting Greenhouse-grown Tomato Plants in Hebei Province
文章编号:
1001-0009（2015）09-0095-04
作者:
孙国珍1; 高利利1; 陆文利2; 王勇3; 张安胜4; 竺晓平1
(1.山东农业大学 植物保护学院，山东 泰安 271018；2.山东农业大学 园艺科学与工程学院，山东 泰安 271018；3.天津市植物保护研究所，天津 300381；4.山东省农业科学院 植物保护研究所，山东 济南 251000)
Author(s):
SUN Guo-zhen1; GAO Li-li1; LU Wen-li2; WANG Yong3; ZHANG An-sheng4; ZHU Xiao-ping1
(1.College of Plant Protection,Shandong Agricultural University,Tai’an，Shandong 271018;2.College of Horticulture Science and Engineering,Shandong Agricultural University,Tai’an,Shandong 271018;3.Tianjin Institute of Plant Protection,Tianjin 300381;4.Institute of Plant Protection,Shandong Academy of Agricultural Sciences,Jinan，Shandong 251000)
关键词:
番茄褪绿病毒; HSP70; CP; RT-PCR检测; 番茄黄化曲叶病毒; 复合侵染
Keywords:
Tomato chlorosis virus; HSP70; CP; RT-PCR detection; Tomato yellow leaf curl virus; mixed infection
分类号:
S 641.2
DOI:
10.11937/bfyy.201509026
文献标志码:
A
摘要:
以2014年夏季在河北7个县市设施番茄种植区田间采集的疑似番茄褪绿病毒(Tomato chlorosis virus，ToCV)感病植株为试材，采用RT-PCR检测、BLAST比对和MEGA 5.0系统树分析等方法，研究河北设施番茄上ToCV的发生和分子鉴定以及ToCV与另一种严重发生的番茄黄化曲叶病毒(Tomato yellow leaf curl virus，TYLC)复合侵染情况。结果表明：以ToCV外壳蛋白(Coat Protein，CP)和热激蛋白(Heat Shock Protein 70，HSP70)共3对特异引物对其中4个典型地区ToCV疑似样品进行检测，分别扩增到约1 032、943、911 bp的特异条带，同时针对ToCV阳性样品利用TYLCV特异引物从部分样品中扩增得到约833 bp的核苷酸序列，4个样品均确定为ToCV阳性，样品与ToCV北京分离物(KC887999) CP基因序列一致性最高，为99.7％，同时确定田间存在ToCV和TYLCV复合侵染。
Abstract:
Taking tomato samples showing symptom similar to that caused by Tomato chlorosis virus (ToCV) found and collected from 7 regions in Hebei Province in the summer of 2014 as material，using RT-PCR detection，BLAST alignment and phylogenetic analysis based on MEGA 5.0 as research methods to detect and identify ToCV occurrence and mixed infection on ToCV and another economic important virus Tomato yellow leaf curl virus (TYLCV).The results showed that three pairs of specific primers targeting the ToCV coat protein (CP) and heat shock protein (HSP70) genes were used for detection，specific 1 032 bp，943 bp and 911 bp fragments were amplified from the symptomatic samples，respectively.The ToCV positive samples were also tested for presence of TYLCV，specific primers of TYLCV were used and a 833 bp specific fragments were amplified and sequenced.Plant samples from Hebei were infected by Tomato chlorosis virus，the sequence analyses showed the nucleotide sequence of Hebei samples had the highest identity of 99.7% with a Beijing isolate of ToCV (KC887999).The mixed infection of ToCV and TYLCV occurred in the field was also confirmed.

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相似文献/References:

[1]代惠洁,刘永光,赵玉翠,等.山东地区番茄褪绿病毒病的发生规律与综合防控策略[J].北方园艺,2017,41(24):211.[doi:10.11937/bfyy.20171697]
　DAI Huijie,LIU Yongguang,ZHAO Yucui,et al.Occurrence and Control Strategies of Tomato Chlorosis Virus Disease in Shandong[J].Northern Horticulture,2017,41(09):211.[doi:10.11937/bfyy.20171697]

备注/Memo

第一作者简介：孙国珍(1989-)，女，山东聊城人，硕士，研究方向为植物病理学。E-mail：178067433@qq.com.

责任作者:竺晓平(1966-)，男，江苏六合人，博士，教授，研究方向为植物病理学。E-mail：zhuxp@sdau.edu.cn.
基金项目：国家公益性(农业)行业科研专项资助项目(201003065)；山东省科技发展计划资助项目(2014GNC111008)；山东省自然科学基金资助项目(ZR2012CM032)。

更新日期/Last Update: 2015-08-01