[1]许忠民,张恩慧,程永安,等.甘蓝胞质雄性不育与内源激素含量的关系[J].北方园艺,2015,39(08):1-5.[doi:10.11937/bfyy.201508001]
　XU Zhong-min,ZHANG En-hui,CHENG Yong-an,et al.Relationships Between Endogenous Hormone Contents and Cytoplasmic Male Sterility in Cabbage[J].Northern Horticulture,2015,39(08):1-5.[doi:10.11937/bfyy.201508001]

点击复制

甘蓝胞质雄性不育与内源激素含量的关系

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第39卷期数: 2015年08 页码: 1-5 栏目: 出版日期: 2015-04-30

Title:: Relationships Between Endogenous Hormone Contents and Cytoplasmic Male Sterility in Cabbage

文章编号:: 1001-0009(2015)08-0001-05

作者:: 许忠民; 张恩慧; 程永安; 马青山; （西北农林科技大学园艺学院，陕西杨凌 712100）

Author(s):: XU Zhong-min; ZHANG En-hui; CHENG Yong-an; MA Qing-shan; (College of Horticulture，Northwest Agricultural and Forestry University，Yangling，Shaanxi 712100)

关键词:: 关键词：甘蓝; 胞质雄性不育; 内源激素

Keywords:: cytoplasmic male sterility; endogenous hormone

分类号:: S 635.1

DOI:: 10.11937/bfyy.201508001

文献标志码:: A

摘要:: 以甘蓝胞质雄性不育系CMS451及其保持系Y03-6为试材，采用间接酶联免疫（ELISA）检测技术，分析花蕾不同发育时期IAA、ABA、GA3和ZR等内源激素的含量与比值变化，研究甘蓝胞质雄性不育与内源激素含量的关系。结果表明：不育系CMS451花蕾GA3和ZR含量均低于保持系，均呈现先升后降；IAA含量不育系均高于保持系，呈现先降后升，保持系则持续减少；ABA含量在小孢子发育中期不育系低于保持系，后期高于保持系，变化趋势相反；综上可知，甘蓝胞质雄性不育系CMS451花蕾发育过程中内源激素的异常变化与小孢子败育有关。

Abstract:: Enzyme linked immunosorbent assays (ELISA) was used as test material，to determine IAA，ABA,ZR and GA₃ contents and the ratio of these endogenous hormones，in order to study the relationship between cabbage cytoplasmic male sterility (CMS)and the content of endogenous hormones.These endogenous hormones were taken from buds of cytoplasmic male sterile line (CMS451)and its maintainer(Y03-6) during reproductive period.The results showed that the content of GA₃?and ZR in buds of CMS451 were lower than those of its maintainer，their contents increased first and decreased afterwards.IAA content of CMS451 was higher than that of its maintainer and decreased first and then increased while it continuously decreased in maintainer.The content of ABA in CMS451 was lower than that of its maintainer at midstage of microspore development，and was higher at the later stage.The results were concluded that,the abnormal hormone change in the bud development of cabbage CMS451 may be related to the microspore abortion.

参考文献/References:

[1]Ogura H.Studies on the new male sterility in Japanese radish with special reference to the utilization of this sterlity towards the practical raising of hybrid s eeds[J].Mern Fac Agri Kagoshima University,1968,6(2):39-78.

[2]Hanson M R.Plant mitochondrial mutations and sterility[J].Annuual Review of Genetics,1991,25:461-486.

[3]孙日飞,方智远,张淑江,等.萝卜胞质大白菜雄性不育系的生化分析[J].园艺学报,2000,27(3):187-192.

[4]Sawhney V K，Shukla A.Male sterility in flowering plants are plant growth substances involved[J].American Journal of Botany,1994,81(12):1640-1647.

[5]刘忠松,官春云,陈社员.植物雄性不育机理的研究及应用[M].北京:中国农业出版社，2001.

[6]史公军,侯喜林,胡巍.细胞质雄性不育白菜败育过程中激素和多胺含量的变化[J].西北植物学报,2004,24(11):2109-2112.

[7]朱玉英,龚静,吴晓光,等.青花菜细胞质雄性不育系叶绿素和内源激素含量变异初探[J].上海农业学报,2002,18(4):42-46.

[8]王华忠,吴则东,韩英,等.甜菜细胞质雄性不育与内源激素含量的关系[J].中国农业科学,2008,41(4):1134-1141.

[9]张明方,陈竹君,汪炳良,等.榨菜胞质雄性不育系和保持系花器发育过程中内源激素变化[J].浙江农业大学学报,1997,23(2):154-157.

[10]夏涛,刘纪麟.生长素和玉米素与玉米细胞质雄性不育性关系研究[J].作物学报,1994,20(1):26-32.

[11]赵玉锦,童哲,陈华君,等.内源植物激素与光敏核不育水稻农垦58S育性的关系[J].植物学报,1996,38(12):936-941.

[12] Shukla A，Sawhney V K.Cytokinins in a genic male sterile line of Brassica napus[J].Physiol Plant,1992,85:23-29.

[13]田长恩,张明永,段俊,等.油菜细胞质雄性不育系极其保持系不同发育阶段内源激素动态变化初探[J].中国农业科学,1998,31(4):20-25.

[14]吴颂如,陈婉芬,周燮.酶联免疫法(ELISA)测定内源植物激素[J].植物生理学通讯,1988(5):53-57.

[15]高夕全，张子学，夏凯，等.雄性不育辣椒中几种内源植物激素的含量变化[J].植物生理学通讯,2001,37(1):31-32.

[16] Shukla A，Sawhney V K.A bscisic acid：one of the factors affecting male sterility in Brassica napu[J].Physiol Plant,1994,91:522-528.

[17] Singh S，Sawhney V K，Pearce D W.Temperature effects on endogenous indole-3 aceticacid levels in leaves and stamens of the normal and male sterile ‘stamenless-2’ mutant of tomato (Lycoperrsicon esculentum Mill)[J].Plant Cell & Enviroment,1992,15:373-377.[

18]唐祈林,荣廷昭,胡长远.不同核背景的玉米CMS系内源激素关系的研究[J].四川农业大学学报,2002,20(3):209-211.

[19]吴智明,胡开林,符积钦,等.辣椒胞质雄性不育与花蕾内源激素含量的关系[J].华南农业大学学报,2010,31(2):1-4.

[20]李英贤,张爱民,黄铁城.小麦细胞质雄性不育与花药组织内源激素的关系[J].农业生物技术学报,1996(4)：307-312.

[21]张子学，罗育淮.辣椒核一质互作雄性不育与叶、蕾中内源激素含量的关系[J].安徽技术师范学院学报,2002,16(2):5-7.

[22]苗锦山,杨文才,刘彩霞,等.葱胞质雄性不育花蕾生化物质含量和能量代谢酶活性的动态变化特征[J].西北植物学报,2010,30(6):1142-1148.

[23]童哲.光敏核不育水稻的发育生物学研究评述[J].植物学报,1998,40(3):189-199.

[24]游年顺,雷捷成,黄利兴,等.水稻同核异质体雄性不育系内源激素IAA、GA_1+3+4+7比较分析[J].福建省农科院学报,1997,12(1):7-11.

[25]黄少白,周燮.水稻细胞质雄性不育与内源GA₁₊₄和IAA的关系[J].华北农学报,1994,9(3):16-20.

[26] Shamon S，Guardia M D.Sex expression and the production of ethylene induced by auxin in the cucumber (Cucumis sarivus L.) [J].Nature,1969,223:186-190.

[27] Polowick P L.Sawhney V K.Microsporogenesis in a normal line and in the Ogu cytoplasmic malesterile line of Brassica napus[J].Sex Plant Report,1990(3):263-276.

[28]任喜波,戴希尧,魏毓棠.不同核背景的萝卜细胞质雄性不育与内源激素关系的研究[J].沈阳农业大学学报,2006,37(2):169-172.

[29]冯忠梅,张凤兰,张德双,等.大白菜新型胞质雄性不育系及其保持系花药不同发育时期内源激素动态变化的研究[J].华北农学报,2005,20(4):40-43.

备注/Memo

第一作者简介：许忠民（1971-），男，陕西大荔人，博士，助理研究员，现主要从事蔬菜种子资源和遗传育种等研究工作。E-mail:xuzhongmin2003@126.com.

基金项目：国家“十一五”科技支撑计划资助项目（2008BADB1B02）；陕西省农业科技攻关计划资助项目（2009K01-24）；西安市农业科技攻关计划资助项目(NC09041（1）)。

收稿日期：2015-01-16

更新日期/Last Update: 2015-07-21