YANG Qing-zhen,LIU De-wang,TIAN Yi-xin,et al.Research Progress on Chemical Structure and Pharmacological Activity of Astragalus Polysaccharide[J].Northern Horticulture,2015,39(07):168-175.[doi:10.11937/bfyy.201507048]
黄芪多糖化学结构与药理作用研究进展
- Title:
- Research Progress on Chemical Structure and Pharmacological Activity of Astragalus Polysaccharide
- 文献标志码:
- A
- 摘要:
- 黄芪多糖（Astragalus polysaccharide,APS）是黄芪主要的活性成分，现代研究表明黄芪多糖有免疫调节、抗衰老、抗肿瘤、抗病毒、降血糖等多种药理作用，这使得黄芪多糖成为业界研究的热点。随着研究的深入，多种黄芪多糖化合物的化学结构及其药理作用被发现。现对黄芪多糖的化学结构和药理作用进行全面的归纳和总结，以期为黄芪多糖的开发及临床应用提供依据。
- Abstract:
- Astragalus polysaccharides (APS) is one of the main active ingredient of Astragli radix.Modern phytochemistry and pharmacological experiments have proved that polysaccharide is one of the major active ingredients in Astragli radix with various important bioactivities，such as immunomodulation，antioxidant，antitumor，anti-diabetes，antiviral，hepatoprotection，anti-inflammation，anti-atherosclerosis，hematopoiesis and neuroprotection.Therefore，the structural features and biological activities of Astragalus polysaccharides become a hot topic in the field of pharmacy.The aim of the present review is to summarize previous and current references and give a comprehensive summary regarding the structural features and biological activities of Astragli radix polysaccharides in order to provide new insight for further development of these macromolecules.
参考文献/References:
[1]国家药典委员会.《中华人民共和国药典》2010年版（1部）[M].北京:中国医药科技出版社,2010:283-284. [2]Jin M L,Zhao K,Huang Q S,et al.Structural features and biological activities of the polysaccharides from Astragalus membranaceus[J].International Journal of Biological Macromolecules,2014,64:257-266. [3]Li R,Chen W C,Wang W P.Extraction，characterization of Astragalus polysaccharides and its immune modulating activities in rats with gastric cancer[J].Carbohydrate Polymers,2009,4(78):738-742. [4]Xiao H B,Liang X M,Lu P C.Classification of structurally related compounds from Astragalus extract by correlation of the logkw and S[J].Chromatographia,2000,5(13):212-220. [5]Liu M,Qin J,Hao Y R,et al.Astragalus polysaccharide suppresses skeletal muscle myostatin expression in diabetes：involvement of ROS-ERK and NF-κB pathways[J].Oxidative Medicine and Cellular Longevity,2014,1(24):497-507. [6]Lian Y H,Xie L L,Chen M H,et al.Effects of an Astragalus polysaccharide and rhein combination on apoptosis in rats with chronic renal failure[J].Evidence-Based Complementary and Alternative Medicine,2014,5(10):862-869. [7]Zhu H Y,Gao Y H,Wang Z Y,et al.Astragalus polysaccharide suppresses the expression of adhesion molecules through the regulation of the p38 MAPK signaling pathway in human cardiac microvascular endothelial cells after ischemia-reperfusion injury[J].Evidence-based Complementary and Alternative Medicine：eCAM,2013,9(17):493-501. [8]Guo L，Bai S P，Zhao L，et al.Astragalus polysaccharide injection integrated with vinorelbine and cisplatin for patients with advanced non-small cell lung cancer：effects on quality of life and survival[J].Medical Oncology,2012,3(29):1656-1662. [9]屈静.低分子量黄芪多糖的分离纯化和结构分析[D].长春:东北师范大学,2010. [10]聂凌鸿,宁正祥.活性多糖的构效关系[J].林产化学与工业,2003(4):89-94. [11]Fu J,Huang L F,Zhang H T,et al.Structural features of a polysaccharide from Astragalus membranaceus (Fisch.) Bge.var.mongholicus (Bge.) Hsiao[J].Journal of Asian Natural Products Research,2013,6(15):687-692. [12]Niu Y G,Wang H Y,Xie Z H,et al.Structural analysis and bioactivity of a polysaccharide from the roots of Astragalus membranaceus (Fisch) Bge.var.mongholicus (Bge.) Hsiao[J].Food Chemistry,2011,3(128):620-626. [13]Yin J Y,Chan B L,Yu H,et al.Separation，structure characterization，conformation and immunomodulating effect of a hyperbranched heteroglycan from Radix astragali[J].Carbohydrate Polymers,2012,1(87):667-675. [14]艾连中,吴艳,郭本恒,等.黄芪多糖的研究进展[J].山东食品发酵,2008(1):39-41. [15]方圣鼎,陈燕,徐小异,等.中药黄芪有效成分的研究Ⅰ多糖体的分离、性质及其生理活性[J].有机化学,1982(1):26-29. [16]方积年.多糖的研究状况[J].药学学报,1986,21(12):944-949. [17]方积年,Wanger H.黄芪葡聚多糖的化学结构[J].化学学报,1988,46:1101-1106. [18]黄乔书,吕归宝,李雅臣,等.黄芪多糖研究[J].药学学报,1982,17(3):200-204. [19]王莹,赵毅民,张起凤,等.黄芪中一种新葡聚糖的分离纯化及化学结构研究[J].中草药,2001,32(11):962-964. [20]李宏全,赵万国,吕小虎,等.黄芪中一种新杂聚多糖的理化分析[J].中国药学杂志,2009,44(9):654-657. [21]林梦感,杨义芳,许海燕.黄芪多糖MAPS-5的化学结构及其体外淋巴细胞增殖活性[J].中草药,2009(12):1865-1868. [22]刘荣强.黄芪多糖结构与抗肿瘤活性研究[D].天津:天津科技大学,2011. [23]刘星谐,王美英,吴厚生,等.黄芪多糖的分离及其免疫活性的研究[J].天然产物研究与开发,1994,16(1):23. [24]Masashi T,Noriko S,Naoko O,et al.A reticuloendothelial system activating glycan from the root s of ast ragalus membranaceus[J].Phytochemistry,1992,31(1):63-65. [25]Li S G,Zhang Y Q.Characterization and renal protective effect of a polysaccharide from Astragalus membranaceus[J].Carbohydrate Polymers,2009,78(2):343-348. [26]邹一愚,顾学裘,陈骐,等.双多糖多相脂质体的研究(1)多糖中有效成分的选择和鉴定[J].沈阳药学院学报,1998,4(3):170-173. [27]单俊杰,王易,翁颖琦,等.黄芪毛状根多糖与黄芪多糖化学组成及免疫活性的比较[J].中草药,2002,33(12):1096-1099. [28]陈艳蕊,毛欣月,金文闻,等.黄芪多糖结构及其单糖组成的气相色谱-质谱研究[J].现代生物医学进展,2011(S1):4632-4635. [29]Kiyohara H,Uchida T,Takakiwa M,et al.Different contributions of side-chainsin β-D-（1-3,6）-galactans on intestinal Peyer’s patch-immunomodulation by polysaccharides from Astragalus mongholics Bunge[J].Phytochemistry,2009,71(2-3):280-293. [30]王志学,马启凤,何群,等.黄芪化学成份的研究[J].沈阳药科大学学报,1983,3(17):53-56. [31]王瑞遵.黄芪多糖的分离纯化及体外抗氧化活性研究[D].郑州:郑州大学,2010. [32]颜军,易勇,邬晓勇,等.黄芪多糖的相对分子量测定及单糖组成分析[J].食品科技,2012(12):278-283. [33]徐诺,巢志茂.黄芪的药性:对体外抗体生成作用的影响[J].国外医学中医中药分册,1998,20(2):40. [34]刘耀,石英,刘职瑞,等.黄芪多糖与黄芪甲苷配伍对辐射损伤模型小鼠的保护作用[J].中国药房,2014(3):211-214. [35]李天一,汪丽佩,吴国琳.黄芪多糖对免疫性肝损伤小鼠免疫调节的影响[J].中国中医急症,2014(1):25-27. [36]刘俊英,曾广仙,熊金蓉,等.黄芪多糖对创伤应激小鼠胸腺、脾脏、淋巴细胞中NF-K B mRNA 与IL-10mRNA表达影响的形态计量学研究[J].中国体视学与图像分析,2004,9(1):21-24. [37]潘惠娟,宋涛.黄芪多糖对抗环磷酰胺免疫抑制作用的实验研究[J].细胞与分子免疫学杂志,1997,13(增刊):54-57. [38]梁华平,张艳,耿波.黄芪多糖对烧伤小鼠细胞免疫功能的影响[J].中华整形烧伤外科杂志,1994,10(2):138-141. [39]Lee K Y,Jeon Y J.Macrophage activation by polysaccharide isolated from Astragalus membranaceus[J].Intlmlnullo Pharmaeology,2005,5:1225-1233. [40]杨五彪.植物多糖对家兔动脉粥样硬化脂质过氧化及炎症反应的干预[J].四川中医,2007,25(2):12-14. [41]吴勇,欧阳静萍,涂淑珍,等.黄芪多糖对动脉粥样硬化内皮细胞损伤的影响[J].湖北中医学院学报,2002(1):21-22. [42]吴德光,李清贤,王彦富,等.黄芪多糖在动脉粥样硬化发展过程中保护性作用的研究[J].临床心血管病杂志,2012(2):147-152. [43]汪海宁.探析黄芪多糖对脂多糖诱导大鼠心肌细胞肥大的影响[J].当代医学,2014(3):24-25. [44]张静,王洪新,杨娟,等.黄芪多糖通过Toll样受体4/核转录因子-кB信号通路抑制异丙肾上腺素诱导的心肌细胞肥大[J].中华高血压杂志,2014(2):157-162. [45]朱海燕.黄芪多糖对人心脏微血管内皮细胞缺氧再复氧损伤后核因子-KB表达的影响[J].辽宁中医杂志,2008,35(1):7-9. [46]朱丹.黄芪多糖对人心脏微血管内皮细胞缺血再灌注损伤影响的研究[D].北京:北京中医药大学,2009. [47]陈立新,朱海燕,朱陵群,等.黄芪多糖对人心脏微血管内皮细胞增殖及再灌注后内皮细胞与中性粒细胞黏附的影响[J].中国组织工程研究与临床康复,2007,11(27):53-82. [48]陈雪辉,魏学娟,王涛,等.黄芪多糖对大鼠胰岛素敏感性和脂肪细胞因子的影响[J].新乡医学院学报,2013(5):356-359. [49]Liu M,Wu K,Mao X,et al.Astragalus polysaccharide improves insulin sensitivity in KKAy mice：regulation of PKB/GLUT4 signaling in skeketal muscle[J].J Ethnopharmocol,2010,127(1):32-37. [50]Wang N,Zhang D,Mao X,et al.Astragalus polysaccharides decreased the expression of PTP1B through relieving ER stress induced activation of ATF6 in a rat model of type 2 diabetes[J].Mol Cell Endocrinol,2009,307:89-98. [51]吴德红,王凤杰,邓娟,等.黄芪多糖对Ⅱ型糖尿病大鼠肝脏AMPK苏氨酸磷酸化的影响[J].微循环学杂志,2009,19(3):1-3. [52]林晓泉,廖海,梁蔚婷.注射用黄芪多糖联合化疗治疗恶性肿瘤的临床疗效观察[J].中药材,2009,32(6):1-10. [53]陈瑾歆,何军,张娟娟,等.黄芪多糖对人肝癌细胞HepG2凋亡相关基因表达的影响[J].中国老年学杂志,2014(1):124-126. [54]吴朝妍,张莹雯,叶太生,等.NF-κB抑制介导的黄芪多糖抗人肺癌细胞效应[J].武汉大学学报(医学版),2013(2):174-177. [55]任美萍,刘明华,李蓉,等.黄芪多糖抗肿瘤活性研究[J].中国新药杂志,2010(3):221-224. [56]梁金花,郑科文,孙立群.探讨中药黄芪多糖对溃疡性结肠炎大鼠肠道菌群失调的调整作用[J].微量元素与健康研究,2013(2):1-3. [57]孙梅.黄芪多糖对LPS损伤小肠上皮细胞的保护作用[J].世界华人消化杂志,2008,16(1):15-17. [58]欧德渊,高铭宇,刘世会,等.黄芪多糖对大肠埃希菌侵入肠上皮细胞的保护研究[J].动物医学进展,2006(8):85-87. [59]钟灵,王振富,文德鉴.黄芪多糖抗衰老作用的实验研究[J].中国应用生理学杂志,2013(4):350-352. [60]赵丹威,欧芹,魏晓东,等.黄芪多糖对衰老HDFβ-半乳糖苷酶活性影响的实验研究[J].中国老年学杂志,2006(10):1361-1362. [61]王振富,钟灵,肖本见,等.黄芪多糖对小鼠益智和低氧/复氧损伤的保护作用[J].中国老年学杂志,2014(4):970-971. [62]刘志洋,万朋,高俊涛.黄芪多糖对血管性痴呆小鼠学习记忆能力的影响[J].吉林医药学院学报,2014(1):14-16. [63]万朋,王师,王军,等.黄芪多糖对小鼠学习记忆能力的影响[J].延边大学医学学报,2011(1):23-25. [64]王胜红,邱斌,徐志红,等.黄芪多糖对内毒素血症性肺损伤治疗作用的实验研究[J].微循环学杂志,2014(1):6-8. [65]李树鹏,郝艳霜,陈福星,等.黄芪多糖和2株益生菌体外抑菌作用研究[J].河南农业科学,2007(4):86-92. [66]李丽娅,凌秋,崔洪波,等.黄芪多糖抗流感病毒的实验研究[J].中国中医药科技,2002,9(6):354-358. [67]吕岫华,刘伟,郝冬梅,等.中药多糖对EB病毒早期抗原激活的抑制作用[J].北京工业大学学报,2010(1):92-97. [68]马宝瑕,陈新,邓军娥.中药多糖研究进展[J].中国医院药学杂志,2003(6):42-44. [69]Yin X L，Chen L，Liu Y，et al.Enhancement of the innate immune response of bladder epithelial cells by Astragalus polysaccharides through 〖JP〗upregulation of TLR4 expression[J].Biochemical and Biophysical Research Communications,2010,2:232-238. [70]Li R，Chen W C，Wang W P，et al.Extraction,characterization of Astragalus polysaccharides and its immune modulating activities in rats with gastric cancer[J].Carbohydrate Polymers,2009,78(4):738-742. [71]Li S G,Zhang Y Q.Characterization and renal Protective effete of a polysaccharide from Astragalus membranaceus[J].Carbohydrate Polymers,2009,78(2):343-348. [72]Kiyohara H，Uchida T，Takakiwa M，et al.Different contributions of side-chains in β-D-(l-3,6)-galactans on intestinal peyer‘s patch-immunomodulation by polysaccharides from Astragalus mongholics Bunge[J].Phytochemistry,2010,71:280-293. [73]田庆锷,李焕德.黄芪多糖抗肿瘤作用研究进展[J].中医药导报,2011(12):86-88. [74]黄皓,干信.天然多糖类活性物质抗乙肝病毒的研究进展[J].中草药,2006(10):1594-1596. [75]胡元亮,孔样峰.10种中药成分对CEF的增殖和抵抗NDV感染的影响[J].畜牧兽医学报,2004,35(3):301-305.
备注/Memo
第一作者简介：杨庆珍（1988-），女，河南新乡人，硕士研究生，研究方向为中药资源及中药新药研究与开发。E-mail:youngqingzhen@126.com. 责任作者：田义新（1963-），男，博士，教授，硕士生导师，现主要从事中药材栽培教学与科研工作。E-mail:y.x.tian2003@163.com. 基金项目：国家自然科学基金面上资助项目(81274013,81473315)；国家自然科学基金重点资助项目(81130069)。