WANG Ze-hua,SHI Bao-cai,GONG Ya-jun,et al.Common Toxicity of Bifenazate and Bifenthrin on the Spider Mite Tetranychus cinnabarinus (Acari：Tetranychidae) Using Bioassay[J].Northern Horticulture,2015,39(07):112-115.[doi:10.11937/bfyy.201507033]
联苯肼酯与联苯菊酯对朱砂叶螨联合毒力的生物测定
- Title:
- Common Toxicity of Bifenazate and Bifenthrin on the Spider Mite Tetranychus cinnabarinus (Acari：Tetranychidae) Using Bioassay
- Keywords:
- Tetranychus cinnabarinus; bifenazate; bifenthrin; common toxicity
- 文献标志码:
- A
- 摘要:
- 以朱砂叶螨为试材，采用玻片浸渍法，研究了2种杀螨剂联苯肼酯和联苯菊酯对朱砂叶螨的联合毒力。结果表明：联苯肼酯和联苯菊酯单独使用时，其24 h对朱砂叶螨的LC50（致死中浓度）分别为23.2385 mg/L和34.5302 mg/L，48 h的LC50分别为16.0384 mg/L和15.3873 mg/L。联苯肼酯和联苯菊酯混配使用时24 h的共毒系数为115~204，48 h共毒系数为134~224，均表现为增效作用。对不同混配比例的分析发现，联苯肼酯与联苯菊酯按有效成分在1∶1的混配比例下较之其它比例增效作用更佳，共毒系数在24 h和48 h时分别为204和221。2种药剂混用能够发挥不同药剂的作用特点，增强杀虫活性，扩大杀虫谱，对延缓抗药性的发展具有重要作用。
- Abstract:
- Taking Tetranychus cinnabarinus as material,using slide-dip method，the effect of common toxicity of bifenazate and bifenthrin on Tetranychus cinnabarinus was studied.The results showed that the lethal concentration (LC50) of bifenazate and bifenthrin to T.cinnabarinus was 23.2385 mg/L and 34.5302 mg/L when used after 24 hours，that was 16.0384 mg/L and 15.3873 mg/L after 48 hours.The CTC (common toxicity coefficient) of the mixture of these two acaracides was 115-204 after 24 hours，while that was to 134-224 after 48 hours，it showed a synergistic toxicity to T.cinnabarinus.Among the different mixture ratios of bifenazate and bifenthrin，the one of 1∶1 showed the highest synergistic toxicity to T.cinnabarinus，with a CTC of 204 and 221 after 24 hours and 48 hours，respectively.A mixture of bifenazate and bifenthrin displayed characteristics of action from both acaracides，enhancing their activity to，expanding their range of，and might be helpful to delay the development of resistance by target pests.
参考文献/References:
[1]Zhang Y Q，Ding W，Zhao Z M，et al.Studies on acaricidal bioactivities of Artemisia annua L.extracts against Tetranychus cinnabarinus Bois.(Acari：Tetranychidae)[J].Agricultural Sciences in China，2008，7(5)：577-584. [2]李隆术，李云瑞.蜱螨学[M].重庆：重庆出版社，1988：4-129. [3]吴孔明，刘孝纯，秦夏卿，等.朱砂叶螨抗药性研究[J].华北农学报，1990，5(2)：117-123. [4]van Leeuwen T，Vontas J，Tsagkarakou A，et al.Acaricide resistance mechanisms in the two-spotted spider mite Tetranychus urticae and other important Acari：A review[J].Insect Biochemistry and Molecular Biology，2010，40(8)：563-572. [5]Guo F Y，Zhang Z Q，Zhao Z M.Pesticide resistance of Tetranychus cinnabarinus (Acari：Tetranychidae) in China：A review[J].Systematic and Applied Acarology，1998，3：3-7. [6]Dekeyser M A，Mcdonald P T，Angle G W.The discovery of bifenazate，a novel carbazate acaricide[J].CHIMIA International Journal for Chemistry，2003，57(11)：702-704. [7]Ochiai N，Mizuno M，Mimori N，et al.Toxicity of bifenazate and its principal active metabolite，diazene，to Tetranychus urticae and Panonychus citri and their relative toxicity to the predaceous mites，Phytoseiulus persimilis and Neoseiulus californicus[J].Experimental and Applied Acarology，2007，43(3)：181-197. [8]James D G.Selectivity of the acaricide，bifenazate，and aphicide，pymetrozine，to spider mite predators in Washington hops[J].International Journal of Acarology，2002，28(2)：175-179. [9]宫亚军，石宝才，王泽华，等.新型杀螨剂-联苯肼酯对二斑叶螨的毒力测定及田间防效[J].农药，2013（3）：23. [10]Ay R，Gürkan M O.Resistance to bifenthrin and resistance mechanisms of different strains of the two-spotted spider mite (Tetranychus urticae)from Turkey[J].Phytoparasitica，2005，33(3)：237-244. [11]Herron G，Rophail J，Wilson L.The development of bifenthrin resistance in two-spotted spider mite (Acari：Tetranychidae) from Australian cotton[J].Experimental and Applied Acarology，2001，25(4)：301-310. [12]王少丽，王然，张友军，等.11 种常用药剂对蔬菜朱砂叶螨的室内毒力测定[J].中国农学通报，2009，25(24)：386-388. [13]Dittrich V，Cranham J，Jepson L，et al.Revised method for spider mites and their eggs (eg Tetranychus spp.and Panonychus ulmi Koch)，FAO method No.10a[J].FAO Plant Production and Protection Paper，1980，21：49-53. [14]Sun Y P，Johnson E.Analysis of joint action of insecticides against house flies[J].Journal of Economic Entomology，1960，53(5)：887-892. [15]陈青，赵冬香，莫圣书，等.阿维菌素与吡虫啉混用对豇豆荚螟的联合毒力[J].农药，2006，44(12)：561-564. [16]邹华娇.联苯菊酯与啶虫脒增效混配制剂对茶假眼小绿叶蝉的防治效果[J].福建农业科技，2012(11)：55-57. [17]van Leeuwen T，Tirry L.Esterase-mediated bifenthrin resistance in a multiresistant strain of the two-spotted spider mite，Tetranychus urticae[J].Pest Management Science，2007，63(2)：150-156. [18]陈秋双，赵舒，邹晶，等.朱砂叶螨抗药性监测[J].应用昆虫学报，2012，49(2)：364-369. [19]王少丽，张友军，徐宝云，等.朱砂叶螨对不同蔬菜寄主的取食选择性[J].环境昆虫学报，2011，33(3)：315-320. [20]彭德良.蔬菜病虫害的综合治理 (十) 蔬菜线虫病害的发生和防治[J].中国蔬菜，1998(4)：57-58.
备注/Memo
第一作者简介：王泽华（1983-），女，硕士，助理研究员，研究方向为农业昆虫与害虫防治。E-mail：wangzehua200707@163.com. 责任作者：魏书军（1981-），男，博士，副研究员，研究方向为农业昆虫与害虫防治。E-mail：shujun268@163.com。基金项目：北京市农林科学院创新能力建设专项资助项目（KJCX20140403，KJCX20150406）；北京市农业科技资助项目（2013010301）。