YANG Zhi-gang,ZHU Dong,LUO Bing,et al.Construction of Fingerprint and Analysis of Genetic Diversity with EST-SSR Markers for Thirty-seven Peony Cultivars in Shandong[J].Northern Horticulture,2015,39(07):77-81.[doi:10.11937/bfyy.201507024]
三十七个山东牡丹品种DNA指纹图谱构建与EST-SSR标记遗传多样性分析
- Title:
- Construction of Fingerprint and Analysis of Genetic Diversity with EST-SSR Markers for Thirty-seven Peony Cultivars in Shandong
- Keywords:
- EST-SSR; DNA fingerprint; genetic diversity; peony
- 文献标志码:
- A
- 摘要:
- 以中国常熟尚湖牡丹园引栽的37个山东牡丹品种为试材，采用筛选出的10对多态性高、稳定性好且在染色体上分布均匀的EST-SSR标记引物，研究山东牡丹37个品种DNA指纹图谱和遗传多样性。结果表明：10对EST-SSR标记引物在37份材料中共扩增出了共计58个多态性基因型，平均每对引物5.8个，变幅2~13种；10对引物的多态性频率（FP）平均值为0.60；筛选出的4对核心引物可以将37个牡丹品种完全区分,以遗传相似系数0.69为阈值可将供试37个试牡丹品种分成5类。EST-SSR标记在供试牡丹品种间多态性差异较大，适合用于牡丹的DNA指纹图谱的构建。
- Abstract:
- Taking thirty-seven peony cultivars from Shandong as experimental materials,the DNA fingerprinting database was successfully constructed,and the genetic diversity was analyzed based on ten evenly distributed EST-SSR primer pairs with high polymorphisms and good repeatability.The results showed that,among the 37 varieties，10 primer pairs had 58 polymorphic locis，and 5.8 polymorphic locis were detected by each EST-SSR primer pair on an average with the range from 2 to 13.EST-SSR markers were suitable for using in construction of peony DNA fingerprint with polymorphism differences between the selected varieties.Thirty-seven cultivars could be identified by 4 primer combinations st least.The genetic deversity analyzed indicated that，37 peony varieties could be divided into five types based on the genetic similarity coefficient of 0.69 threshold.
参考文献/References:
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备注/Memo
第一作者简介：杨志刚（1977-），男，硕士，高级实验师，研究方向为植物功能基因研究与开发。E-mail：zhigangyang77@sina.cn. 基金项目：江苏农业科技支撑资助项目（BE2011445）；苏州市科技计划资助项目（SZS201102，SYN201110，SNG201323）；江苏省大学生实践创新训练计划资助项目（201310333032Y）；苏州市吴江区科技计划资助项目（WN201311，WN201317）；常熟市科技计划资助项目（CS201205）。