WU Hai-yan,ZHANG Ji,GAO Qing-ya,et al.Effect of Compound Biological Preparation on Cold Resistance of Brassica campetris(rape) ‘Longyou 9’ Seedling[J].Northern Horticulture,2015,39(06):1-5.[doi:10.11937/bfyy.201506001]
复合外源物质对“陇油9号”白菜型油菜幼苗抗寒性的影响
- Title:
- Effect of Compound Biological Preparation on Cold Resistance of Brassica campetris(rape) ‘Longyou 9’ Seedling
- 关键词:
- “陇油9号”白菜型油菜; 复合外源物质; 抗寒性
- 文献标志码:
- A
- 摘要:
- 以“陇油9号”白菜型油菜幼苗为试材,利用低温培养箱模拟低温条件,通过叶面喷施复合外源物质,研究了复合外源物质对白菜型油菜幼苗抗寒性的影响。结果表明:以抗寒性植物水提液为基液配置的复合外源物质处理可使油菜幼苗在低温(0±1)℃胁迫下保持相对较高的超氧化物歧化酶(SOD)活性和葡萄糖、可溶性蛋白质、叶绿素、脯氨酸(Pro)含量,减少丙二醛(MDA)含量的积累,提高了“陇油9号”白菜型油菜幼苗的抗寒性;复合外源物质可在一定程度上提高“陇油9号”白菜型油菜幼苗的抗寒能力,有效降低“陇油9号”白菜型油菜幼苗的冻害。
- Abstract:
- The Brassica campetris(rape)‘Longyou 9’ seedling was selected as experimental material,and the low temperature conditions were stimulated with low temperature incubator.The Brassica campetris(rape)‘Longyou 9’ seedling were treated with compound biological preparation as foliar application to study the effect of low temperature stress on it.The results showed that the composite exogenous substances of cold resistance of plant water solution for the base configuration treatment could make the rape seedlings under the stress of low temperature (0±1)℃ maintain a relatively high superoxide dismutase (SOD)activity and the contents of glucose,soluble protein,chlorophyll and proline (Pro),reduce the malondialdehyde (MDA)accumulation,improve the cold resistance of Brassica campetris(rape)‘Longyou 9’.Composite exogenous substances could increase the Brassica campetris(rape)‘Longyou 9’ seedlings fight cold ability,effectively reduce Brassica campetris(rape)‘Longyou 9’ seedling frost damage.
参考文献/References:
[1]Deng J M,Jian L C.Advances of studies on plant freezing-tolerance mechanism:freezing tolerance gene expression and its function[J].Chinese Bulletin of Botany,2001,18(5):521-530. [2]Lee D H,Lee C B.Chilling stress-induced changes of antioxidant enzymes in the leaves of cucumber:in gelenzyme activity assays[J].Plant Science,2000,159(1):75-85. [3]徐正华,张晓红,陈秀斌,等.不同栽培措施对油菜抗寒性的影响[J].华中农业大学学报,2012,31(6):661-667. [4]李世成,牛俊义,盖珥,等.零上低温处理对不同甘蓝型油菜品种抗寒性的影响[J].干旱地区农业研究,2007(6):40-44. [5]Morrison M J,Andrews C J.Variable increases in cold hardiness induce in winter rape by plant growth regulators[J].Journal of Plant Growth Regulation,1992,11:113-117. [6]马霓,刘丹,张春雷,等.植物生长调节剂对油菜生长及冻害后光合作用和产量的调控效应[J].作物学报,2009,35(7):1336-1343. [7]徐呈祥.提高植物抗寒性的机理研究进展[J].生态学报,2012,32(24):7966-7980. [8]王学奎.植物生理生化实验原理和技术[M].2版.北京:高等教育出版社,2006. [9]张琳,赵晓嫚,任君,等.外源ABA对低温胁迫下青花菜植株生长及若干生理特性的影响[J].福建农业学报,2012,27(3):267-271. [10]Kerepesi I,Bányai-stefanovitsé,Galiba G.Cold acclimation and abscisic acid induced alterations in carbohydrate content in call of wheat genotypes differing in frost tolerance [J].Journal of Plant Physiology,2004,161(1):131-133. [11]Zuther E,Büchel K,Hundertmark M,et al.The role of raffi nose in the cold acclimation response of Arabidopsis thaliana[J].FEBS Letters,2004,576:169-173. [12]骆爱玲,刘家尧,马德钦,等.转甜菜碱醛脱氢酶基因烟草叶片中抗氧化酶活性增高[J].科学通报,2000,45(18):1953-1956. [13]和红云,田丽萍,薛琳.植物抗寒性生理生化研究进展[J].天津农业科学,2007,13(2):10-13. [14]黄翔.复合外源活性物质对几种作物抗寒性的影响及其机理研究[D].武汉:华中农业大学,2008. [15]Arbaoui M,Balko C,Link W.Study of faba bean (Vicia faba L.) winter-hardiness and development of screening methods[J].Field Crops Research,2008,106:60-67. [16]孙玉洁.植物动力2003抗寒机理的研究以及油菜抗寒剂的研制[D].长沙:湖南农业大学,2011. [17]王敏,曲存民,刘晓兰,等.温度胁迫下甘蓝型油菜苗期生理生化指标的研究[J].作物杂志,2013(2):53-59. [18]沈漫,王明麻,黄敏仁.植物抗寒机理研究进展[J].植物学通报,1997,14(2):1-8. [19]朱惠霞,孙万仓.白菜型冬油菜的抗寒性及其生理生化特性[J].西北农业学报,2007,16(4):34-38.
备注/Memo
第一作者简介:吴海燕(1979-),女,甘肃渭源人,硕士研究生,研究方向为植物资源开发与利用。E-mail:1285916629@qq.com.责任作者:张继(1963-),女,博士,研究员,现主要从事植物资源开发与利用等研究工作。E-mail:1083455950@qq.com.基金项目:国家自然科学基金资助项目(31200255);国家“十二五”科技支撑计划资助项目(2012BAD20B06)。