HUANG Ting,GONG Ling-ling,JIANG Jun-xi,et al.Identification of the Pathogen Causing Anthraconose of Melon (Cucumis melo L.)[J].Northern Horticulture,2015,39(05):132-134.[doi:10.11937/bfyy.201505038]
甜瓜炭疽病病原菌鉴定
- Title:
- Identification of the Pathogen Causing Anthraconose of Melon (Cucumis melo L.)
- 文献标志码:
- A
- 摘要:
- 以南昌郊蛟桥镇甜瓜基地的甜瓜炭疽病病果为试材，采用致病性测定、形态学观察和分子生物学的方法，研究了甜瓜炭疽病病原归属。结果表明：黄瓜炭疽病病原菌形态具典型炭疽菌属类真菌Colletorichum orbiculare的特征，GPDH基因序列与NCBI-GenBank中已有的C.orbiculare序列同源性为100%，接种发病症状与自然发病症状类似，因此将该病原菌鉴定为瓜类刺盘孢（Colletorichum orbiculare）。
- Abstract:
- Taking the disease melon of anthracnose from Jiaoqiao town in Nanchang for test material，the pathogen was identified by the study of morphology，pathogenicity，cultural feature and the sequence of GPDH.The results showed that the sequence of GPDH of isolate NCBI showed a sequence identity of 100% with C.orbiculare published in GenBank.The vaccination symptoms was similar to the filed symptoms，so the fungus was identified as C.orbiculare.
参考文献/References:
[1] John C G，Liu B，James C C，et al.Charaterization of diversity in Colletorichum acutatum sensu lato by sequence analysis of two gene introns，mtDNA and intron RFLPs，and mating compatibility[J].Mycologia，2003，95(5):872-895. [2] 方中达.植病研究方法[M].2版.北京：农业出版社，1979. [3] Kuan C P，Wu M T，Huang H C，et al.Rapid detection of Colletotrichum lagenarium causal agent of anthracnose of cucurbitaceous crops by PCR and Real-time PCR[J].Journal of Phytopathology，2011，159 (4)：276-282. [4] Tsay J G，Chen R S，Wang W L，et al.First report of anthracnose on cucurbitaceous crops caused by Glomerella magna in Taiwan[J].Plant Disease，2010，94(6)：787.
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备注/Memo
第一作者简介：黄婷（1989-），女，硕士，研究方向为分子植物病理学。E-mail:jxfzncht@126.com. 责任作者：蒋军喜（1964-），男，博士，教授，现主要从事植物病害综合治理等研究工作。E-mail:jxau2011@126.com. 基金项目：江西省教育厅科学技术研究资助项目（GJJ13269）。