WANG Pei-dan,XIE Chang-hai,SU De-wei,et al.Application of Juncao Spent Mushroom Substrate Extracts on the Mother Culture Media of Dictyophora indusiata (Pers.)[J].Northern Horticulture,2015,39(05):155-159.[doi:10.11937/bfyy.201505045]
菌草菌糠提取物在竹荪母种培养基中的应用
- Title:
- Application of Juncao Spent Mushroom Substrate Extracts on the Mother Culture Media of Dictyophora indusiata (Pers.)
- Keywords:
- Juncao; spent mushroom substrate; extracts; D.indusiata; mother culture media
- 文献标志码:
- A
- 摘要:
- 以平菇、香菇、灵芝、真姬菇、银耳、杏鲍菇、金针菇7种常见菌草菌糠浸提液配制的培养基为试材,研究了其对竹荪菌丝生长的影响,进而对最适竹荪菌丝生长的菌糠进行水溶物质的提取。将该菌糠提取物配制的不同浓度梯度的培养基与PDA培养基进行对比,筛选出最适的菌糠提取物添加量,旨在研究出一种新式竹荪母种培养基。结果表明:以菌草灵芝菌糠浸提液作为培养基,竹荪菌丝生长速度快于其它菌糠与PDA培养基(P<0.01)。当向母种培养基中添加6 g/L菌草灵芝菌糠提取物时,最适于竹荪菌丝的快速生长,且菌丝粗壮、洁白。
- Abstract:
- Spent mushroom substrate (SMS) from Pleurotus ostreatus,Lentinus edodes,Ganoderma lucidum, Hypsizygus marmoreus, Tremella fuciformis, Pleurotus eryngii and Flammulina velutipes that cultivated by Juncao technology were used for the materials selection in producing mother culture media for the cultivation of D.indusiata mycelium.Water-soluble substance of SMS that can promote the rapid growth of D.indusiata mycelium were extracted.Then,culture media added with different ratio of water-soluble extracts were used in growing mycelium of D.indusiata,and then the appropriate dose of SMS extracts were screened in order to get a new formula of mother cultrue media of D.indusiata,compared with general PDA media.The results showed that water-soluble extracts from SMS of G.lucidum performed the best effect in growth of D.indusiata mycelium,and the latter grew faster compared with other spent mushroom substrates (P<0.01).D.indusiata mycelium grew faster and showed better quality while 6 g extracts from SMS G.lucidum were added to 1 L mother media.
参考文献/References:
[1] 林占熺.菌草学[M].3版.北京:国家行政学院出版社,2013. [2] 吴振忠,吴潇.菌草菌糠的研究开发和利用[J].食用菌,2010,32(2):4-5. [3] 郁建强.浅谈食用菌菌糠的综合利用[J].再生资源研究,1997(5):34. [4] 蒋冬花,郑重.食用菌的代谢产物[J].生物学杂志,2000,17(4):1-4. [5] 刘文波,赵勇,孙晓红,等.竹荪提取物对食源性细菌的抑菌特性研究[J].微生物学杂志,2013(4):50-54. [6] 刘芳,陈娇,吴三林,等.红托竹荪母种培养基配方的筛选试验[J].乐山师范学院学报,2010(12):21-23. [7] 杨勇,张凤英,陈岑.PDA培养基改良配方的研究[J].酿酒科技,2012(4):29-31. [8] 梁明勤,海建平,陈世昌,等.杏鲍菇菌糠对鸡腿菇菌丝生长和产量的影响[J].河南农业科学,2012(10):118-121. [9] 曾丽平.银耳废料栽培鸡腿蘑效益分析与关键技术[J].食用菌,2005,27(2):17-18. [10]马国良,刘卫东.白灵菇废料栽培鸡腿菇技术[J].食用菌,2005,27(3):28-29. [11]刘军,王照亮.灵芝菌糠料栽培平菇技术[J].食用菌,2000,22(5):25-26. [12]耿小丽,刘宇,王守现,等.废料栽培平菇试验[J].中国食用菌,2010,29(2):19-20. [13]龚振杰,赵桂云.木耳菌糠袋栽平菇技术[J].北方园艺,2009(3):214-215. [14]许绍朴.袋栽香菇菌棒废料再利用研究简报[J].食用菌学报,1995,2(4):55-58. [15]赵桂云,马怀良.平菇菌糠提取液对四种食用菌菌丝生长的影响[J].北方园艺,2010(22):170-171. [16]曾荣耀,郑林用,罗霞.蕈菌次生代谢产物中的活性成分[J].中国食用菌,2008,27(1):43-45. [17]姜明,荆伟,陈鑫.不同比例滑菇菌糠提取液对几种食用菌菌丝生长的影响[J].黑龙江农业科学,2014(5):113-115. [18]李用芳.食用菌菌渣的再利用[J].生物学通报,2001,36(3):44-45. [19]王贺祥.食用菌学[M].北京:中国农业大学出版社,2004:41-43. [20]李俐俐,刘天学.平菇菌糠提取液对5种食用菌菌丝生长的影响[J].安徽农业科学,2007,35(2):430,432. [21]陈恒雷,王轶群,万红贵,等.阿魏菇菌糠成分测定及对四种大宗食用菌化感效应研究[J].北方园艺,2009(5):210-212. [22]李挺,宋斌,林敏,等.真姬菇菌糠提取液对5种食用菌的化感作用[J].中国食用菌,2013,32(1):38-40. [23]王金贺,王延锋,孙靖轩,等.不同比例的黑木耳菌糠提取液对五种蘑菇菌丝生长的影响[J].北方园艺,2013(4):156-158.
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备注/Memo
第一作者简介:王培丹(1987),女,硕士研究生,现主要从事食药用菌等研究工作。E-mail:603554582@qq.com. 责任作者:林占熺(1943-),男,研究员,博士生导师,现主要从事食药用菌等研究工作。基金项目:福建省科技重大专项资助项目(2012NZ0002);科技部国家中心组建资助项目(2011FU125X11)。