ZAN Li-feng,LIANG Rui-juan,BAO Hai-ying.Antioxidant and Antimicrobial Activities of Inonotus hispidus Extracts[J].Northern Horticulture,2015,39(05):151-155.[doi:10.11937/bfyy.201505044]
粗毛纤孔菌提取物的抗氧化和抑菌活性研究
- Title:
- Antioxidant and Antimicrobial Activities of Inonotus hispidus Extracts
- Keywords:
- Inonotus hispidus; mushroom; antioxidant; antimicrobial activity
- 文献标志码:
- A
- 摘要:
- 采用福林酚法和NaNO2-Al(NO3)3比色法分别测定了粗毛纤孔菌子实体70%乙醇提取物中的多酚和黄酮，含量分别为（64.02±0.73）μg/mg和（5.74±0.13）μg/mg；并对70%乙醇提取物及主要化合物Hispidin的抗氧化和抑菌活性进行了测定。结果表明：70%乙醇提取物和Hispidin具有强烈的清除DPPH和?OH自由基活性的作用，在浓度为200 mg/kg时清除率分别与BHA相当，清除率达到90%左右；采用K-B纸片扩散法测定了70%乙醇提取物及Hispidin对大肠杆菌Escherichia coli ATCC8099、金黄色葡萄球菌Staphyloccocus aureus ATCC6538、枯草芽孢杆菌Bacillus subtilis的抑制活性，结果除70%乙醇提取物高剂量（40.96 mg/mL）对大肠杆菌的抑制率达到40.35%，其余均没有明显活性。
- Abstract:
- Antioxidant capacity and antimicrobial activities of Inonotus hispidus extracts obtained with 70% ethanol and compound hispidin were investigated by Folin-Ciocalteu and NaNO2-Al(NO3)3 colorimetric methods.Total phenolics were (64.02±0.73) μg/mg (gallic acid equivalent) while the flavanoids were (5.74±0.13) μg/mg (rutin equivalent) in the extract.The antioxidant and antibacterial activities of 70% ethanol extracts and hispidin were determined.The results showed that DPPH and ?OH free radical-scavenging activities were found to exhibit about 90% inhibition，at concentration of 200 mg/kg.Positive correlations were found between total phenolic content in the mushroom extracts and their antioxidant activities.70% ethanol extract and hispidin showed narrow antibacterial activities against Staphyloccocus aureus and Bacillus subtilis.70% ethanol extracts inhibited the growth of the Escherichia coli，and inhibition values was 40.35% at concentration of 40.96 mg/mL.
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备注/Memo
第一作者简介：昝立峰（1980-），男，博士，讲师，现主要从事食药用菌质量评价等研究工作。E-mail：tengfei007zlf@126.com. 基金项目：邯郸市科技局资助项目（1422104057-3）；邯郸学院食用菌研究所资助项目；国家自然科学基金面上资助项目（31270088）。