WANG Jie-fei,CAO Zhi-min,BAI Lu-chao,et al.Identification of Phytophthora Species Causing Pricklyash Blight from Shaanxi and Gansu Provinces and Screening of Antagonistic Actinomycetes[J].Northern Horticulture,2015,39(05):113-119.[doi:10.11937/bfyy.201505034]
陕甘两省花椒疫霉病病原鉴定及其拮抗菌筛选
- Title:
- Identification of Phytophthora Species Causing Pricklyash Blight from Shaanxi and Gansu Provinces and Screening of Antagonistic Actinomycetes
- Keywords:
- pricklyash; Phytophthora; morphology; rDNA-ITS; β-tubulin; pathogenicity; antagonism
- 文献标志码:
- A
- 摘要:
- 以采自陕西、甘肃的花椒疫霉病菌为试材，依据病原菌的菌落与繁殖体特征、rDNA-ITS及β-tubulin基因序列分析和致病性测定，研究该病菌的种类与分布，并对花椒疫霉菌拮抗菌进行了筛选。结果表明：陕西宝鸡地区的疫霉菌菌系Pfs3、Pfs4、Pbjt和Pfs5为苎麻疫霉（Phytophthora boehmeriae Sawada）；甘肃陇南地区菌系Pcsx、Pwmc、Pwqh、Pwbs3和Pwbs2为多寄主疫霉（Phytophthora multivora Scott & Jung），从花椒园土壤中分离筛选出1株对疫霉菌具有拮抗效果的放线菌zy-1，初步鉴定为链霉菌之一种（Streptomyces sp.）。
- Abstract:
- Taking species and distribution of Phytophthora which were isolated from pricklyash (Zanthoxylum bungeanum) in Shaanxi and Gansu provices as material，based on colony，sporangium and oogonium morphology，ITS and β-tubulin gene sequences and their pathogenicity，species and distribution of the bacteria were studied，and the antagonistic bacteria of pricklyash Phytophthora were screened.The results showed that Phytophthora isolates Pfs3，Pfs4，Pbjt and Pfs5 from Baoji of Shaanxi were identified as Phytophthora boehmeriae Sawada，and isolates Pcsx，Pwmc，Pwqh，Pwbs3 and Pwbs2 from Longnan of Gansu were identified as Phytophthora multivora Scott and Jung.One actinomycetes isolated from the soil of pricklyash field was screened out，and preliminary identified as Streptomyces sp.which showed antagonistic activity to these Phytophthora species.
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备注/Memo
第一作者简介：王洁菲（1987-），女，陕西西安人，硕士，研究方向为森林菌物学。E-mail：hiwjf0932@126.com. 责任作者：曹支敏 (1954-)，男，陕西富平人，教授，博士生导师，现主要从事森林病理学与菌物学等研究工作。E-mail：zmcao@nwsuaf.edu.cn. 基金项目：国家“十二五”科技支撑计划资助项目（2012BAD19B0804）。