QI Fei,LIU Guan,LI Jing-fu.QTL Analysis for Total Organic Acid Content in Tomato Fruit[J].Northern Horticulture,2015,39(05):109-113.[doi:10.11937/bfyy.201505033]
番茄果实有机酸总含量的QTL分析
- Title:
- QTL Analysis for Total Organic Acid Content in Tomato Fruit
- Keywords:
- ; tomato FONT-SIZE: 10.5pt">tomato： FONT-SIZE: 10.5pt">：acid content' target="_blank" rel="external"> FONT-SIZE: 10.5pt">acid content; QTL; AFLP; SSR
- 文献标志码:
- A
- 摘要:
- 以番茄高酸品种“12680”为母本，低酸品种“12574”为父本，配制杂交组合，构建含有260个单株的F2代群体为试材，采用酸碱滴定法测量果实的总酸含量，并进行遗传图谱构建及QTL分析，研究番茄果实中有机酸含量的遗传规律并找到与有机酸含量相关的QTL位点。结果表明：在256对AFLP引物和185对SSR引物中获得多态性良好的AFLP引物24对，SSR引物17对；构建连锁图谱，该图谱包括6个连锁群，共42个标记，覆盖基因组长度1 358.93 cM，检测LOD值大于2.5的标记2个，贡献率分别为20.27%、10.98%，均分布在第2连锁群上。其中Ta1为负向加性效应，Ta2为正向加性效应。
- Abstract:
- An AFLP and SSR genetic linkage map was constructed in tomato with a 260 intraspecific F2 population derived from a cross between high acid content line ‘12680’ as female parent and low acid contend line ‘12574’ as male parent.Total acid content was determined by acid-base titration,total organic acid genetic development was studied to find QTL position related to organic acid conted.The results showed that, in totally chose 256 paris of AFLP molecule markers，185 paris of SSR molecule markers,24 paris of AFLP molecule markers and 17 paris of SSR molecule markers had favorable polymorphism.F2 generation were analyzed for patterns to construct the six linkage groups.It contained 41 molecule markers，the total length of 1 358.93 cM.There were two markers detected the LOD scores greater than 2.5，genetic contribution rate were 20.27% and 10.98%.The 2 QTLs distributed in 2 linkage groups，among which Ta1 was negative additive effects,Ta2 was positive additive effect.
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备注/Memo
第一作者简介：戚飞（1990-），女，硕士研究生，研究方向为蔬菜育种。E-mail:1036860306@qq.com. 责任作者：李景富（1943-），男，教授，博士生导师，研究方向为蔬菜遗传与育种及生物技术。E-mail：lijf_2005@163.com. 基金项目：国家自然科学基金资助项目（NSFC 31272171）。