YANG Jin,YU Zhen-wen,LI Zi-qin,et al.Physiological and Biochemical Changes to Salt Stress in Hydropically Cultured Salix psammophila Seedlings[J].Northern Horticulture,2015,39(05):71-78.[doi:10.11937/bfyy.201505024]
盐胁迫下沙柳幼苗的生理生化变化
- Title:
- Physiological and Biochemical Changes to Salt Stress in Hydropically Cultured Salix psammophila Seedlings
- Keywords:
- morphological changes; enzyme solution macroscopic changes; enzyme activity; endogenous hormones
- 文献标志码:
- A
- 摘要:
- 以沙柳幼苗为试材，研究了0（对照）、50、100、150 mM NaCl胁迫对沙柳幼苗的形态变化、酶液宏观变化、酶活性变化及内源激素含量变化的影响。结果表明：100 mM NaCl盐浓度及以下胁迫对沙柳幼苗近乎无影响，ABA、IAA、GA的含量相对平稳，无太大变化，通过测定内源激素的含量可发现，100 mM NaCl盐浓度时沙柳幼苗长势出现下降，100 mM NaCl盐浓度以上的胁迫对沙柳幼苗的影响显著，沙柳幼苗所能承受NaCl迫害的最高Na盐浓度处于100 mM NaCl和150 mM NaCl之间。150 mM NaCl盐浓度胁迫时，其在第1天和第3天间代谢紊乱程度达到其可耐受的极限程度，随后的几天植株走向死亡。同时沙柳幼苗在濒临死亡时其ABA含量无大幅度升高，这与常见植物有所不同。而IAA、GA的含量呈现出在短时间内迅速达到最大含量，随后的几天内含量有所降低。在极高浓度的NaCl胁迫下，沙柳幼苗的SOD活性在短时间内迅速下降后持续大幅度上升，沙柳幼苗死亡时，SOD活性达到最高。沙柳幼苗受到NaCl胁迫时，其CAT比SOD和POD敏感，CAT活性受到的影响更显著。
- Abstract:
- Taking Salix psammophila seedlings as test materials，the effect of NaCl concentration (0 mM（CK），50 mM，100 mM and 150 mM) on morphological changes and the activities of SOD，CAT，POD，and endogenous hormone content of Salix psammophila seedlings were measured in this study.The results showed that the growth was strongly depressed by 100 mM or less NaCl concentration.Contents of ABA，IAA，GA3 were relatively stable.By measuring content of endogenous hormones,it was found that the Salix psammophila seedlings growth decreased under 100 mM NaCl salt stress，the impact of more than 100 mM NaCl concentration salt stress on Salix psammophila seedling was significant，the highest NaCl concentration that the cuttings could withstand was between 100 mM and 150 mM.Under 150 mM NaCl salt treatment，Salix psammophila seedlings between the first day and three days of metabolic disorders extent reached its limit of tolerable degree，the following days the plants gradually died.When the Salix psammophila seedlings at the brink of death，which did not increase significantly in ABA content，this was different from the common plants.The IAA，GA3 showed in a short time quickly reached maximum content，their contents were reduced in the next few days.Under high concentrations of NaCl stress，the SOD activity decreased rapidly in a short period of time，and later continued substantial increase，when Salix psammophila seedling died，the SOD activity reached the highest.When the Salix psammophila seedlings were stressed by NaCl，its CAT was more sensitive than SOD and POD，the CAT activity been affected more significantly.
参考文献/References:
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备注/Memo
第一作者简介：杨进（1984-），男，硕士，助理实验师，现主要从事园林植物的引种等研究工作。E-mail：1521155822@qq.com.