YAN Qiu-jie,HUANG Dan,GU Ying-qiong,et al.ffect of Exogenous Calcium to Disporum cautoniense Leaves Growth at Natural Low Temperature[J].Northern Horticulture,2015,39(03):66-69.[doi:10.11937/bfyy.201503021]
外源钙对自然低温下富贵竹叶片生长的影响
- Title:
- ffect of Exogenous Calcium to Disporum cautoniense Leaves Growth at Natural Low Temperature
- Keywords:
- Disporum cautoniense; natural low temperature; calcium chloride; relative electrical conductivity; MDA; free proline
- 文献标志码:
- A
- 摘要:
- 为探究能够提高富贵竹叶片生长的适宜外源钙浓度,用水培法研究了冬季自然低温下0.3%~0.7%氯化钙溶液对富贵竹叶片黄叶面积及黄叶指数,叶片干尖率及叶片含水量、叶绿素含量、丙二醛含量、游离脯氨酸含量及电导率等形态和生理指标的影响。结果表明:0.3%氯化钙溶液处理下,富贵竹叶片的黄叶面积,叶片干尖率和叶绿素b含量低于对照,黄叶指数高于对照,但均与对照无显著差异;叶片含水量极显著地高于对照;叶绿素a含量、相对电导率、丙二醛含量和游离脯氨酸含量极显著地低于对照,但与对照的差值最小。综合各项指标,0.3%的氯化钙溶液优于0.4%~0.7%氯化钙处理浓度。因此,0.3%氯化钙溶液有利于自然低温下水培富贵竹叶片的生长。
- Abstract:
- In order to explore the suitable exogenous calcium concentration for the better growth of Disporum cautoniense leaves,the morphological and physiological indexes of Disporum cautoniense cultured in 0.3%~0.7% calcium chloride solution at the natural low temperature of winter were measured.The results showed that the yellow leaf area,the rate of yellow blade tip and chlorophyll b content of the calcium chloride solution treatment under 0.3% were lower than the control,and the yellow leaf index were higher than the control.There were no significant difference between the above index and the control.The leaf water content was higher than the control very significantly.The chlorophyll a content,the relative electrical conductivity,MDA content and the free proline content were lower than the control very significantly,but the differences with the control attached minimum.From all indexes,the 0.3% of calcium chloride solution was better than 0.4%~0.7% of calcium chloride solution concentration.Therefore,the suitable exogenous calcium chloride concentration to Disporum cautoniense leaf growth at natural low temperature was 0.3%.
参考文献/References:
[1]张新权.富贵竹的盆栽与养护[J].江西园艺,2004(1):35-36.
[2]王茂桦,陈暮初,危疆泽.水杨酸对富贵竹抗寒生理的影响[J].四川林业科技,2009,30(6):6-11.
[3]毛文岭,王勇,刘绣华.富贵竹研究进展[J].济源职业技术学院学报,2010,9(4):29-30.
[4]徐华,杨学奎.富贵竹水插繁殖技术[J].现代园艺,2010(10):26-27. [5]林思锻,李焕苓,赖钟雄,等.富贵竹的顶梢扦插生根[J].亚热带农业研究,2008,4(4):265-267.
[6]范昭鹤.富贵竹的愈伤组织诱导与植株再生试验研究[J].园艺博览,2009(1):16-17.
[7]Kudla J,Xu Q,Harter K,et al.Genes for calcineurin B-like proteins in Arabidopsis are differentially regulated by stress signals[J].Proceedings of the National Academy of Sciences of the United States of America,1999,96:4718-4723.
[8]王笑笑,葛水莲,陈建中,等.植物体内钙离子的感受器类型及功能研究[J].河北农业科学,2008(11):57-58.
[9]龚伟,王伯初.钙离子在植物抵抗非生物胁迫中的作用[J].生命的化学,2011,31(1):107-111.
[10]张志良,瞿伟箐,李小芳.植物生理学实验指导[M].4版.北京:高等教育出版社,2009.
[11]陈爱葵,韩瑞宏,李东洋,等.植物叶片相对电导率测定方法比较研究[J].广东教育学院学报,2010,30(5):89-91.
[12]李绍军,龚月桦,王俊儒,等.关于茚三酮法测定脯氨酸含量中脯氨酸与茚三酮反应之探讨[J].植物生理学通讯,2005,41(3):365-368.
[13]何云,李宪伟.大蒜生产中黄叶干尖现象研究[J].农业科技与信息,2002(9):25-26.
[14]刘慧春,朱开元,周江华,等.常绿水生鸢尾叶片含水量与抗寒性初报[J].江苏农业科学,2010(1):182-183.
[15]壬毅,杨宏福,李树德.园艺植物冷害和抗冷性的研究[J].园艺学报,1994,21(3):239-244.
[16]王增池,孔德平,梁风琴,等.萘乙酸浓度对金边富贵竹水培繁殖效果的影响[J].河南农业科学,2007,11(4):31-32.
[17]白青华,郭晓冬,王萍,等.低温对辣椒幼苗叶片氮及叶绿素含量的影响[J].甘肃农业大学学报,2009(6):48-51,78.
[18]田丹青,葛亚英,潘刚敏,等.低温胁迫对3个红掌品种叶片形态和生理特性的影响[J].园艺学报,2011,38(6):1173-1179.
[19]Chance B,Maehly A C.Assay of catalase and peroxidase[J].Methods Enzymol,1955,2:764-775.
[20]McCord J M,Fridovich I.Superoxide dismutase:An enzymic function for ekythrocuprein[J].Journal of Biological Chemistry,1960,224:6049-6055.
[21]Hare P D,Cress W A.Metabolic implications of stress induced proline accumulation in plants[J].Journal of Plant Growth Regulation,1997,21:79-102.
[22]龚明,李英,曹宗巽.植物体内的钙信使系统[J].植物学通报,1990,7(3):19-29.
[23]余叔文.植物生理与分子生物学[M].北京:科学出版社,1992:728-733,762.
[24]Xu Y,Huystee R B.Association of calcium and calmodulin to peroidase secretion and activation [J].Plant Physiology,1993,141(2):141-146.
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备注/Memo
第一作者简介:闫秋洁(1980-),女,甘肃酒泉人,博士研究生,讲师,现主要从事植物抗逆遗传等研究工作。E-mail:veronica008@163.com. 责任作者:杨毅(1963-),男,四川彭州人,博士,教授,现主要从事植物抗逆等研究工作。基金项目:国家自然科学基金资助项目(31171586)。