[1]李 森,李婷婷,亢秀萍,等.耧斗菜园艺品种与野生华北耧斗菜[J].北方园艺,2015,39(02):98-101.[doi:10.11937/bfyy.201502027]
　LI Sen,LI Ting-ting,KANG Xiu-ping,et al.The SRAP Analysis on the Genetic Relationship of Wild Aquilegia yabeana and Cultivated Aquilegia caerulea[J].Northern Horticulture,2015,39(02):98-101.[doi:10.11937/bfyy.201502027]
点击复制

耧斗菜园艺品种与野生华北耧斗菜

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第39卷 期数: 2015年02 页码: 98-101 栏目: 出版日期: 2015-01-30
Title:
The SRAP Analysis on the Genetic Relationship of Wild Aquilegia yabeana and Cultivated Aquilegia caerulea
作者:
李 森; 李婷婷; 亢秀萍; 邢国明
(山西农业大学 园艺学院，山西 太谷 030801)
Author(s):
LI Sen; LI Ting-ting; KANG Xiu-ping; XING Guo-ming
(College of Horticulture，Shanxi Agricultural University，Taigu，Shanxi，030801)
关键词:
华北耧斗菜; 亲缘关系; SRAP分析
Keywords:
Aquilegia yabeana; genetic relationship; SRAP analysis
DOI:
10.11937/bfyy.201502027
文献标志码:
A
摘要:
以采自山西省沁水县历山等8处植物多样性丰富的山区10个华北耧斗菜材料和5个耧斗菜园艺品种为试材，采用SRAP(sequence-related amplified polymorphism)分子标记技术进行了亲缘关系分析。结果表明：从88对引物中筛选出17对多态性丰富的引物组合用于PCR扩增，得到扩增条带352条，其中多态性条带307条，多态性比率为87.2%。利用NTSYSpc-2.0软件进行UPGMA聚类分析，分别得出15个供试材料与10个华北耧斗菜材料间的亲缘关系树状图。15个供试材料的相似系数为0.6416～0.8571，聚类结果遵循植物学分类。10个华北耧斗菜材料的相似系数为0.7218～0.8571，生境是影响华北耧斗菜聚类结果的主要因素，地理分布为次要因素。
Abstract:
The genetic relationship among 10 wild samples of Aquilegia yabeana from 8 main mountains in Shanxi province and 5 horticulture cultivars of Aquilegia caerulea were analyzed with the method of SRAP Marker.The results showed that 17 pair primes among 88 were selected for PCR，and 352 clear amplified bands were obtained.The total number of polymorphism bands was amounted to 307，the polymorphism rate of genetic was 87.2%.The 15 samples’ matrix of genetic similarity coefficient was calculated by the software of NTSYSpc-2.0，and the clustering dendrograms were obtained for 15 samples and 10 samples of Aquilegia yabeana.The similarity coefficient of 15 samples was between 0.6416-0.8571，the samples had more relationship with botanical classification.The similarity coefficient of the 10 samples of Aquilegia yabeana was between 0.7218-0.8571，and the clustering result of these samples has more relationship with the environment and geographical distribution.

参考文献/References:

[1]朱蕊蕊,杨姗姗,明晓,等.耧斗菜愈伤组织和不定芽诱导的初步研究[J].江西农业学报，2009,21(7):81-82.

[2]吕晋慧,杨玉芳.山西野生观赏植物种质资源及其利用研究[J].山西农业科学，2007，35(6)：12-15.
[3]滕红梅,姚建信,李鸿俊,等.山西南部野生花卉种质资源调查研究[J].运城学院学报,2008,26(2):41-47.
[4]明军,张启翔,毛庆山,等.‘美人’梅与其近缘种亲缘关系的AFLP研究[J].园艺学报,2002,29(6)：588-589.
[5]刘青林,陈俊愉.梅花亲缘关系RAPD研究初报[J].北京林业大学学报，1999,21(5):81-85.
[6]戴思兰,陈俊愉,李文彬.菊花起源的RAPD分析[J].植物学报,1998,40(11):110-116.
[7]赵宣,周志钦,林启冰,等.芍药属牡丹组(Paeonia sect.Moutan)种间关系的分子证据:GPAT基因的PCR-RFLP和序列分析[J].植物分类学报，2004，42(3)：236-244.
[8]陈向明,郑国生,孟丽.玫瑰、月季、蔷薇等蔷薇属植物RAPD分析[J].园艺学报，2002，29(1):78-80.
[9]王亚玲,李勇,张寿洲.几种玉兰亚属植物的RAPD亲缘关系分析[J].园艺学报,2003，30(3):299-302.
[10]苏友波,林春,毛静,等.香石竹品种的RAPD标记[J].园艺学报,2004,31(1):109-111.
[11]Li G,Quiros C F.Sequence-related amplifed polymorphism(SRAP),A new marker system based on a simple PCR reaction：its application to mapping and gene in Brassica[J].Theoretical and Applied Genetics，2003,107(1)：168-180.
[12]邱英雄,胡邵庆,陈跃磊,等.ISSR-PCR技术在桂华品种分类研究中的应用[J].园艺学报,2004,31(4):529-532.
[13]张西西,徐进.万寿菊杂交一代遗传多态性的SRAP标记分析[J].园艺学报,2008,35(8):1221-1226.
[14]Ferriol M,Pico B,Nuez F.Genetic diversity of a germplasm collection of cucurbita pepo using SRAP and AFLP markers[J].Theoretical and Applied Genetics,2003,107:271-282.
[15]Ferriol M，Pico B,Pascual Femandez de Cordova,Nuez F.Molecular diversity of a gemplasm collection of squash(Cucurbita moschata) determined by SRAP and AFLP marker[J].Crop Science,2004,44:653-664.[16]高丽霞,胡秀.中国姜花属基于SRAP分子标记的聚类分析[J].植物分类学报，2008,46(6):899-905.

相似文献/References:

[1]王鸿磊,丁 强,吕 蔚,等.我国双孢菇菌株与国外引进菌株间亲缘关系的ISSR分析[J].北方园艺,2014,38(11):96.
　WANG Hong-lei,DING qiang,LV Wei,et al.Genetic Relationship Based on ISSR Markers Among Agaricus bisporus Strains From China and Overseas[J].Northern Horticulture,2014,38(02):96.
[2]张玉平,姜明,鲁绍伟,等.黑莓亲缘关系的AFLP分析[J].北方园艺,2013,37(21):116.
　ZHANG Yu-ping,JIANG Ming,LU Shao-wei,et al.Genetic Relationship Analysis of Blackberries by AFLP[J].Northern Horticulture,2013,37(02):116.
[3]鲁敏,汤浩茹,罗娅,等.SSR标记技术在梨属植物亲缘关系研究中的应用[J].北方园艺,2012,36(23):193.
　LU Min,TANG Hao-ru,LUO Ya,et al.The Applied Research Progression of SSR in Studying Parentage Relationship of Pyrus L.[J].Northern Horticulture,2012,36(02):193.
[4]马丽,王玉海,明东风.石榴种质资源过氧化物酶同工酶的亲缘关系分析[J].北方园艺,2012,36(19):124.
　MA Li,WANG Yu-hai,MING Dong-feng.Analysis of Genetic Relationship for Punica granatum L.Germplasm Based on Peroxidase Isozyme[J].Northern Horticulture,2012,36(02):124.
[5]韩山师范学院 生物系,广东 潮州 0.粤东地区不同橄榄品种叶片解剖结构比较[J].北方园艺,2012,36(13):24.
　CHEN Dan-sheng,ZHUANG Dong-hong,MA Rui-jun,et al.Comparison on the Anatomical Leaf Structure of Different Chinese Olive Cultivars in Eastern Guangdong[J].Northern Horticulture,2012,36(02):24.
[6]高强,王惠林,郑健,等.父母本与多倍体西瓜着色秕籽的相关性研究[J].北方园艺,2014,38(18):22.
　GAO Qiang,WANG Hui-lin,ZHENG Jian,et al.Study on Correlation Between Parents and Colored Aborted Seed of Polyploid Watermelon[J].Northern Horticulture,2014,38(02):22.
[7]童俊,周媛,毛静,等.鸢尾属部分园艺品种遗传多样性的ISSR分析[J].北方园艺,2015,39(10):104.[doi:10.11937/bfyy.201510025]
　TONG Jun,ZHOU Yuan,MAO Jing,et al.Genetic Diversity Analysis of Iris L.Germplasm by ISSR[J].Northern Horticulture,2015,39(02):104.[doi:10.11937/bfyy.201510025]
[8]赵英,黄国弟,付海天,等.十二份杧果种质亲缘关系的SRAP分析[J].北方园艺,2015,39(21):99.[doi:10.11937/bfyy.201521026]
　ZHAO Ying,HUANG Guodi,FU Haitian,et al.SRAP Analysis of 12 Mango Germplasm Genetic Relationships[J].Northern Horticulture,2015,39(02):99.[doi:10.11937/bfyy.201521026]
[9]郭印山,牛早柱,石广丽,等.基于SSR分子标记的葡萄品种遗传多样性分析[J].北方园艺,2016,40(07):89.[doi:10.11937/bfyy.201607023]
　GUO Yinshan,NIU Zaozhu,SHI Guangli,et al.Genetic Diversity Analysis of Grape Resources Based on SSR Markers[J].Northern Horticulture,2016,40(02):89.[doi:10.11937/bfyy.201607023]
[10]牛红云,王 臣,薛贵彬,等.四种寒带睡莲的杂交亲合性研究[J].北方园艺,2011,35(02):0.[doi:10.11937/bfyy.201102037]
　NU Hong-yun,WANG Chen,XTIE Gui-bina,et al.Study on Affinity of Sexual Hybridizationin Four Taxa of Nymphae Northeast[J].Northern Horticulture,2011,35(02):0.[doi:10.11937/bfyy.201102037]

备注/Memo

第一作者简介：李森(1982-)，男，博士研究生，讲师，研究方向为园艺植物种质资源利用与创新。E-mail：saulisen@163.com.基金项目：山西省重大科技攻关资助项目（20080311010-1）；山西农业大学青年基金资助项目（201002）。

更新日期/Last Update: 2015-02-24