LI Xu,CAO Wan-wan,JIANG Dan,et al.Study on Sex Identification of Actinidia arguta by Morphology and Physiological Biochemical[J].Northern Horticulture,2014,38(24):6-9.
基于形态观察法和生理生化法的软枣猕猴桃
- Title:
- Study on Sex Identification of Actinidia arguta by Morphology and Physiological Biochemical
- 文章编号:
- 1001-0009(2014)24-0006-04
- Keywords:
- Actinidia arguta; sex identification; morphology observation; physiological biochemical index
- 分类号:
- S 663.4
- 文献标志码:
- A
- 摘要:
- 以软枣猕猴桃雌雄株休眠枝和新梢为试材，采用形态观察法和生理生化法对软枣猕猴桃植株的性别进行了鉴定研究。结果表明：软枣猕猴桃雌株的叶痕间距和皮孔密度显著大于雄株；组培苗雄株显著高于雌株，根系数量显著低于雌株；BTB染色法鉴别软枣猕猴桃性别的反应时间为4 h以后，但以反应4 h差异最明显；TTC染色法是鉴定成龄软枣猕猴桃雌雄株的有效方法，但是否适用于童期还有待进一步探讨。
- Abstract:
- Taking male and female plants of Actinidia arguta dormant branches and shoots as experimental materials,with the method of morphology observation and physiological biochemical index determination for the Actinidia arguta plant gender identification were studied.The results showed that,leaf scars spacing and lenticel density of female plants were significantly greater than the male plants in Actinidia arguta；tissue culture seedling height of male plants was significantly higher than female plants；root number of male plants was significantly lower than female plants；BTB staining method to identify Actinidia arguta gender reaction after 4 hours,but the most obvious difference appear at 4 hours；TTC staining method was an effective method for the identification between male and female plants of Actinidia arguta,whether it was suitable for the juvenile phase remains to be further discussed.
参考文献/References:
[1]
陕西省果协赴澳考察组
.
新西兰猕猴桃考察报告
[J].
果农之友
,2009(8):36-37.
[2]Bugala W.Sex determioation of poplars from the color of leaves[J].Forestry Abstracts,1951,52(2):13-16. [3]丁之恩.银杏[M].北京:中国林业出版社,1999:39-40. [4]罗晶.沙棘植株性别的判断[J].中国水土保持,1986(Ⅱ):46. [5]徐东生,华光安,刘殊,等.猕猴桃雌雄识别的多元统计分析[J].武汉植物学研究,1998,16(3):283-284. [6]赵林森,徐锡增.复叶槭雌雄株叶片中水溶性酚类物质的比较分析[J].新疆农业大学学报,1998(3):229-232. [7]李国梁,林柏年,沈德绪.酚类物质在鉴别园艺雌雄性植物中的应用研究[J].园艺学报,1993,20(4):397-398. [8]曹宗粪.植物的性别分化及其控制[J].生物学通报,1965(2):4-7. [9]杨俊杰,张天翔,蔡坤秀,等.用溴麝香草酚蓝鉴别芦笋成年植株的雌雄性[J].福建热作科技,2008,4(33):18-22. [10]曲超,胡兰英,字肖萌,等.栝楼雌雄株形态、生理生化指标差异的研究[J].种子,2010,29(10):4-7. [11]李广旭,沈永波,高艳敏,等.皮孔组织结构及密度与苹果枝干粗皮病发生的关系[J].果树学报,2005,21(4):350-353. [12]龙程,潘瑞炽.用溴麝香草酚蓝鉴定石刁柏(芦笋)雌雄株[J].植物生理学通讯,1991,27(3):204-205. [13]张雄.用“TTC”法(红四氮唑)测定小麦根和花粉的活力及其应用[J].植物生理学通讯,1982,48(3):50. [14]Seo J H,Hwang Y S,Chun J P,et al.Changes of phenolic compounds and occurrence of skin browning,and characterization of cartially purified polyphenol oxidases in oriental pear fruits[J].Journal-Korean Society For Horticultural Science,2001,42(2):184-188. [15]李珊,程舟,李彦,等.雌雄栝楼植株内含物比较研究[J].中草药,2008,39(2):260-263.
相似文献/References:
[1]曾照旭,朴一龙,金东淳,等.1-甲基环丙烯处理对软枣猕猴桃果实软化的影响[J].北方园艺,2014,38(01):123.
ZENG Zhao-xu,PIAO Yi-long,JIN Dong-chun,et al.Effects of 1-MCP Treatment on Softening of Actinidia arguta Fruit[J].Northern Horticulture,2014,38(24):123.
[2]王宁,杨玉红,康宗利,等.软枣猕猴桃植株的过氧化物酶同工酶酶谱差异研究[J].北方园艺,2012,36(04):120.
WANG Ning,YANG Yu-hong,KANG Zong-li,et al.Study on the Differences of Peroxidase Isozyme Electrophoretogram in Plants of Actinidia arguta[J].Northern Horticulture,2012,36(24):120.
[3]刘延吉,郝桂杰,姜岩岩,等.不同品种野生软枣猕猴桃最佳组培方法的研究[J].北方园艺,2012,36(17):124.
LIU Yan-ji,HAO Gui-jie,JIANG Yan-yan,et al.Study on the Optimal Method of Tissue Culture on Different Species of Wild Actinidia arguta[J].Northern Horticulture,2012,36(24):124.
[4]温 钢,刘海燕,杨 梅.软枣猕猴桃黄酮的提取及体外抗氧化研究[J].北方园艺,2015,39(04):140.[doi:10.11937/bfyy.201504033]
WEN Gang,LIU Hai-yan,YANG Mei.Extraction of Flavonoids from Actinidia arguta and Its Antioxidant Activity in vitro[J].Northern Horticulture,2015,39(24):140.[doi:10.11937/bfyy.201504033]
[5]李旭,曹万万,姜丹,等.长白山野生软枣猕猴桃资源分布与果实和叶片性状多样性[J].北方园艺,2015,39(15):22.[doi:10.11937/bfyy.201515005]
LI Xu,CAO Wanwan,JIANG Dan,et al.Resource Distribution and Character Diversity of Fruit and Leaf of Wild Actinidia arguta From Changbai Mountain Area[J].Northern Horticulture,2015,39(24):22.[doi:10.11937/bfyy.201515005]
[6]刘 延 吉１,耿 书１,田 晓 艳 ２.辽宁地区五种野生软枣猕猴桃 Ｒ Ａ Ｐ Ｄ 遗传多样性分析[J].北方园艺,2010,34(18):0.[doi:10.11937/bfyy.201018052]
,Ｔ ｈ ｅ Ａ ｎ ａ ｌ ｙ ｓ ｉ ｓ ｏ ｆ Ｇ ｅ ｎ ｅ ｔ ｉ ｃ Ｄ ｉ ｖ ｅ ｒ ｓ ｉ ｔ ｙ ｏ ｎ Ｒ ａ ｎ ｄ ｏ ｍ Ａ ｍ ｐ ｌ ｉ ｆ ｉ ｅ ｄ Ｐ ｏ ｌ ｙ ｍ ｏ ｒ ｐ ｈ ｉ ｃ Ｄ Ｎ Ａｏ ｆ Ｆ ｉ ｖ ｅ Ａ ｃ ｔ ｉ ｎ ｉ ｄ ｉ ａ ａ ｒ ｇ ｕ ｔ ａ Ｓ ｐ ｅ ｃ ｉ ｅ ｓ ｉ ｎ Ｌ ｉ ａ ｏ ｎ ｉ ｎ ｇ Ｐ ｒ ｏ ｖ ｉ ｎ ｃ ｅ[J].Northern Horticulture,2010,34(24):0.[doi:10.11937/bfyy.201018052]
[7]朴 一 龙,朴 日 子.野生软枣猕猴桃离体培养体系的建立及优化[J].北方园艺,2011,35(15):0.[doi:10.11937/bfyy.2011015059]
.Ｅ ｓ ｔ ａ ｂ ｌ ｉ ｓ ｈ ｍ ｅ ｎ ｔ ａ ｎ ｄ Ｏ ｐ ｔ ｉ ｍ ｉ ｚ ａ ｔ ｉ ｏ ｎ ｏ ｆ ｔ ｈ ｅ Ｔ ｉ ｓ ｓ ｕ ｅ Ｃ ｕ ｌ ｔ ｕ ｒ ｅ Ｓ ｙ ｓ ｔ ｅ ｍ ｏ ｆ Ｗ ｉ ｌ ｄ Ａ ｃ ｔ ｉ ｎ ｉ ｄ ｉ ａ ａ ｒ ｇ ｕ ｌ ａ[J].Northern Horticulture,2011,35(24):0.[doi:10.11937/bfyy.2011015059]
[8]杨义明,赵淑兰,艾 军,等.大果软枣猕猴桃优系‘840l’选育初报[J].北方园艺,2011,35(02):0.[doi:10.11937/bfyy.201102065]
YANG Yi-ming,ZHAO Shtrlan,Al Jun,et al.Preliminary Study on Excellent Ctdtivar of ’ 8401 ’ of Actinidia arguta P!anch[J].Northern Horticulture,2011,35(24):0.[doi:10.11937/bfyy.201102065]
[9]朴 一 龙,赵 兰 花.软 枣 猕 猴 桃 研 究 进 展[J].北方园艺,2008,32(03):0.[doi:10.11937/bfyy.200803032]
[J].Northern Horticulture,2008,32(24):0.[doi:10.11937/bfyy.200803032]
[10]王颖,刘嘉仪,马志,等.软枣猕猴桃绿枝扦插生根效果[J].北方园艺,2016,40(24):22.[doi:10.11937/bfyy.201624006]
WANG Ying,LIU Jiayi,MA Zhi,et al.Orthogonal Experiment Optimization of Actinidia arguta in Rooting Effect on Soft-wood Cuttage[J].Northern Horticulture,2016,40(24):22.[doi:10.11937/bfyy.201624006]
备注/Memo
第一作者简介：李旭（1989-），男，黑龙江兰西人，硕士研究生，研究方向为果树栽培生理。E-mail：ybu09yllixu@163.com.