[1]朴 锦,王 坤,范惠明,等.关苍术的开花动态和花蕊形态及花粉活力研究[J].北方园艺,2014,38(12):142-145.
　PIAO Jin,WANG Kun,FAN Hui-ming,et al.Reseach on Flowering Dynamic,Flower Morphology and Pollen Viability of Atractylodes japonica [J].Northern Horticulture,2014,38(12):142-145.
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关苍术的开花动态和花蕊形态及花粉活力研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第38卷 期数: 2014年12期 页码: 142-145 栏目: 出版日期: 2014-06-30
Title:
Reseach on Flowering Dynamic,Flower Morphology and Pollen Viability of Atractylodes japonica
文章编号:
1001-0009(2014)12-0142-04
作者:
朴 锦1; 2; 王 坤2; 范惠明3; 王永明3
1.沈阳农业大学 园艺学院，辽宁 沈阳 110000；
2.延边大学 农学院，吉林 延吉 133002；
3.吉林省延边长白山药业有限公司，吉林 汪清 133200
Author(s):
PIAO Jin1; 2; WANG Kun2; FAN Hui-ming3; WANG Yong-ming3
1.College of Horticulture,Shenyang Agricultural University,Shenyang,Liaoning 110000;
2.Agricultural College,Yanbian University,Yanji,Jilin 133002;
3.Jilin Yanbian Changbai Mountain Pharmaceutical Co.Ltd,Wangqing,Jilin 133200
关键词:
关苍术; 开花动态; 花粉活力; 花粉; 柱头
Keywords:
Atractylodes japonica; flowering dynamic; pollen viability; pollen; stigma
分类号:
S 567. 239
文献标志码:
A
摘要:
以关苍术为试材，通过挂牌调查、扫描电镜观察及花粉培养等方法，研究了关苍术开花动态、花蕊形态及花粉活力。结果表明：关苍术单株花序的开放顺序是自上而下，花序为由外至内，雌花花期为12~14 d，两性花花期为14~17 d；关苍术花粉最长轴为57.95±2.44 μm，属于大孢粉，花粉粒形态指数为1.19，属于长球形；检测花粉活力的染色法中以联苯胺-过氧化氢染色为最好，适宜的花粉培养条件为蔗糖浓度15%、培养温度25℃、培养时间4 h，此条件下花粉萌发率达80.4%。
Abstract:
Taking Atractylodes japonica as material,the flowering dynamics of rhizoma atractylodis,flower morphology and pollen viability were studied by listing survey,scanning electron microscope and the methods of pollen culture.The results showed that the opening sequence of a single plant inflorescence of Atractylodes japonica was from top to bottom,inflorescence inside was from outside to inside,the florescence of female flower was 12~14 d,the florescence of bisexual flower was 14~17 d;the pollen longest axis of Atractylodes japonica was 57.95±2.44 μm and was the large pollen,pollen morphology index was 1.19 and was the long ball shape;Benzidine-hydrogen peroxide staining method was the best for staining of pollen viability detection.The most suitable pollen culture conditions was when sucrose concentration was 15%,culture temperature was 25℃,culture time was 4 h,the pollen germination rate of rhizoma atractylodis was 80.4% under this condition.

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相似文献/References:

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备注/Memo

第一作者简介：朴锦（1973-），女，博士研究生，副教授，研究方向为中药材栽培及育种。E-mail：piaojin@ybu.edu.cn.
基金项目：延边大学校级资助项目（2011800-602013127）。
收稿日期：2014-01-16

更新日期/Last Update: 2014-08-02