ZHANG Yan-zhen,XIAO Zhi-xin,PU Yong,et al.Study on Antifungal Activity of Four Plant Extracts on Alternaria alternata[J].Northern Horticulture,2014,38(11):108-110.
四种植物提取物对烟草赤星病菌抑菌活性研究
- Title:
- Study on Antifungal Activity of Four Plant Extracts on Alternaria alternata
- 文章编号:
- 1001-0009(2014)11-0108-03
- Keywords:
- plant extracts; Alternaria alternate; antifungal activity
- 分类号:
- S 435. 72
- 文献标志码:
- A
- 摘要:
- 以烟草赤星病菌为供试靶标,采用孢子萌发及生长速率测定法,对华山姜、毗黎勒、团花树和金钮扣的95%乙醇粗提物进行了抑菌活性测定。结果表明:在终浓度为200 μg/mL下,华山姜对该菌的孢子萌发及菌丝生长的抑制率最高,分别为72%、53%,其次为毗黎勒。团花树和金钮扣只对该菌的孢子萌发有低抑制活性。因此,华山姜对烟草赤星病菌孢子萌发及菌丝生长的抑制效果显著优于另外3种提取物,与75%百菌清WP阳性对照在相同浓度下达到同一显著水平。
- Abstract:
- Using Alternaria alternata as the trail target,the antifungal activity of 95% ethanol crude extract from Alpinia chinensis,Terminalia bellirica,Anthocephalus chinensis,Spilanthes paniculata were studied by the spore germination and growth rate method.The results showed that Alpinia chinensis had the highest inhibitory rate for spore germination and mycelial growth,the inhibition rate were 72% and 53% respectively at the concentration of 200 μg/mL,followed by Terminalia bellirica.The extracts of Anthocephalus chinensis and Spilanthes paniculata only had low inhibitory activities against the spores of Alternaria alternata.Therefore,at the same concentration,the extract of Alpinia chinensis showed equivalent strong inhibitory effects with the positive control of 75% Chlorothalonil WP,and significantly stronger than the other three extracts.
参考文献/References:
[1] 关博元.重庆烟草赤星病菌种级地位及致病力分化研究[D].重庆:西南大学,2007.
[2] Durrant W E,Dong X.Systemic acquired resistance[J].Annual Review of Phytopathology,2004,42:185-209.
[3] Liu T,Li L,Wang Y,et al.Differential expression profiles of Alternaria alternate genes in response to carbonyl sulfide fumigation[J].The Journal of Microbiology,2010,48(4):480-485.
[4] Tsuge T,Harimoto Y,Akimitsu K,et al.Host-selective toxins produced by the plant pathogenic fungus Alternaria alternata[J].FEMS Microbiology Reviews,2013,37(1):44-66.
[5] 程丹丹,孙学娟,高辉远,等.烟草赤星病菌代谢产物诱导的烟草BY-2细胞ROS爆发和ATP损耗[J].中国农业科学,2011,44(8):1610-1617.
[6] Simmons E G.Alternaria:An identification manual[M].Utrecht:CBS Fungal Biodiversity Centre,2007:1-585.
[7] Dilip,Monga D.Reaction of some Motibari Tobacco Against Brown Spot Disease[J].Tobacco Research,1989,15(2):138-140.
[8] Monga D.Laboratory Evaluation of Fungicides Against Alternaria alternata (Fries) Keissler Causing Brown Spot of Motibari Tobacco CN[J].Tobacco Research,1990,16(1):61-64.
[9] 祖艳青,蒋士君,王海涛,等.河南省烟草赤星病病原鉴定[J].中国烟草学报,2013,19(4):73-77.
[10] 廉立慧,高丽君,杨娜,等.烟草赤星病菌拮抗性放线菌Q14的筛选及鉴定[J].生物技术通报,2013(10):127-130.
[11] 陈雪,万科,张传萍,等.烟草赤星病拮抗细菌的筛选,鉴定及机制初步研究[J].生物学通报,2013,48(7):51-54.
[12] 马志远.烟草赤星病菌拮抗芽孢杆菌的筛选、鉴定及应用研究[D].杨凌:西北农林科技大学,2012.
[13] 周建国.防治烟草赤星病药剂筛选研究[D].长沙:湖南农业大学,2012.
[14] 易龙,肖崇刚,马冠华,等.防治烟草赤星病有益内生细菌的筛选及抑菌作用[J].微生物学报,2004,44(1):19-22.
[15] 江绍玫.植物源无公害农药研究开发现状[J].江西农业大学学报,2000,22(1):140-142.
[16] 卢金清,王琴,何冬黎,等.两种植物源农药对烟草赤星病的药效试验[J].湖北农业科学,2011,50(6):1155-1157.
[17] 杨晓楠,王猛,申瑞平,等.微孔板法检测番茄灰霉病菌对杀菌剂的敏感性[J].中国农业科学,2012,45(15):3075-3082.
[18] 张伟,廖静静,朱贵李,等.8种植物挥发物和浸提液对三七根腐病菌的抑制活性研究[J].中国农学通报,2013,29(30):197-201.
[19] 周会明,柴红梅,赵静,等.基于SPSS的杨柳田头菇菌丝生长速度与交配型相关性分析[J].西南农业学报,2010,23(6):1992-1998.
[20] 邓业成,毕秀莲,杨林林,等.红树植物苦郎树提取物及其活性成分对植物病原真菌的抑菌活性研究[J].湖北农业科学,2012,51(10):2010-2013.
[21] 曹静,段英姿,马艳芝.菊花提取物对番茄晚疫病菌抑制效果的研究[J].北方园艺,2009(2):111-112.
相似文献/References:
[1]周梦娇,万春鹏,陈金印.植物提取物对柑橘采后病害抑制及保鲜效果的研究进展[J].北方园艺,2014,38(02):186.
ZHOU Meng-jiao,WAN Chun-peng,CHEN Jin-yin.Research Progress on Inhibition of Citrus Postharvest Diseases and Preservation Effects of Plant Extraction[J].Northern Horticulture,2014,38(11):186.
[2]刘胜贵,李路,左清清,等.悬钩子属植物提取物的抑菌效果及抗氧化性研究[J].北方园艺,2014,38(06):128.
LIU Sheng-gui,LI Lu,ZUO Qing-qing,et al.Study on Antimicrobial and Antioxidant Activity of the Extract of Rubus Spp.[J].Northern Horticulture,2014,38(11):128.
[3]武月红.六种蒿属植物提取物抑菌效果的比较研究[J].北方园艺,2014,38(19):121.
WU Yue-hong.Comparative Study on Antibacterial Effect About Plant Extracts of Six Species of Artemisia L. Plants[J].Northern Horticulture,2014,38(11):121.
[4]贺润丽,樊杰,平莉莉,等.不同植物提取物对款冬蚜虫杀虫活性研究[J].北方园艺,2015,39(16):119.[doi:10.11937/bfyy.201516028]
HE Runli,FAN Jie,PING Lili,et al.Insecticidal Activity of the Extracts From Different Plants Against Coltsfoot Aphids[J].Northern Horticulture,2015,39(11):119.[doi:10.11937/bfyy.201516028]
[5]和 玉 华,杨 婧,谢 雯 颖,等.微量点滴法测定植物提取物对绣线菊蚜的触杀作用[J].北方园艺,2010,34(04):0.[doi:10.11937/bfyy.201004060]
HE Yu - hua,YANG Jing,XIE Wen- ying,et al.The Contact Toxicity of Plant Extracts on Aphiscitricola by Micro - drip[J].Northern Horticulture,2010,34(11):0.[doi:10.11937/bfyy.201004060]
[6]和 玉 华,孟 梦.植物提取物对甘蓝蚜影响的研究[J].北方园艺,2009,33(03):0.[doi:10.11937/bfyy.200903017]
HE Yu -hua,MENG Meng.The Study of Plant Extracts on Brevicoryne Brassicae[J].Northern Horticulture,2009,33(11):0.[doi:10.11937/bfyy.200903017]
[7]高小宽,梁魁景,张志强,等.三种植物提取物对梨储藏期黑斑病菌抑制作用[J].北方园艺,2018,42(02):161.[doi:10.11937/bfyy.20171630]
GAO Xiaokuan,LIANG Kuijing,ZHANG Zhiqiang,et al.Inhibitory Activities of Three Kinds of Traditional Plant Medicine Extracts Against Mycelial Growth and Conidial Germination of Alternaria kikuchiana[J].Northern Horticulture,2018,42(11):161.[doi:10.11937/bfyy.20171630]
[8]张宝清,翟雅鑫,薛丽芳,等.四季秋海棠炭疽病菌生物学特性及生防因子的筛选[J].北方园艺,2021,(10):66.[doi:10.11937/bfyy.20202508]
ZHANG Baoqing,ZHAI Yaxin,XUE Lifang,et al.Biological Characteristic of Colletotrichum truncatum on Begonia semperflorens and Screening of Biocontrol Factors[J].Northern Horticulture,2021,(11):66.[doi:10.11937/bfyy.20202508]
备注/Memo
第一作者简介:张焱珍(1986-),女,硕士,助教,研究方向为植物病理学。E-mail:421154119@qq.com.
责任作者:李家瑞(1964-),男,本科,研究员,研究方向为天然产物研发及应用技术研究。E-mail:jiaruili@hotmail.com.
基金项目:国家烟草专卖局中烟办资助项目(2012[200]110201202015);
云南省烟草专卖局资助项目(云烟司[2012]263-2012YN11,[2012]263-2012YN08)。
收稿日期:2014-01-21