ZHOU Hui-ming,ZHANG Yan-zhen,XI Ya-li,et al.Screening of Different Carbon and Nitrogen Sources and Suitable for Fermentation on Five Strains of Lyophyllum decastes[J].Northern Horticulture,2014,38(09):159-162.
五株荷叶离褶伞碳氮源及适合发酵菌株的筛选
- Title:
- Screening of Different Carbon and Nitrogen Sources and Suitable for Fermentation on Five Strains of Lyophyllum decastes
- 文章编号:
- 1001-0009(2014)09-0159-04
- Keywords:
- Lyophyllum decastes; submerged culture; carbon source; nitrogen source; mycelia biomass; exopolysaccharid
- 分类号:
- S 646.19
- 文献标志码:
- A
- 摘要:
- 以荷叶离褶伞为试材，以菌丝体生物量和胞外多糖产量为指标，分别采用不同碳源和氮源对5株荷叶离褶伞菌株进行单因素液体发酵试验，以期筛选荷叶离褶伞液体培养最适碳源、氮源及适合发酵菌株，并为探索其工厂化发酵奠定理论基础。结果表明：菌株ZY01、ZY02 及ZY03的最适碳源是蔗糖，最适氮源是蛋白胨，其次为麸皮、尿素；菌株ZY04、ZY05以菌丝体生物量为指标的最适碳源是葡萄糖，最适氮源是蛋白胨，以胞外多糖产量为指标的最适碳源是蔗糖，最适氮源分别是尿素、蛋白胨；菌株ZY02的菌丝体生物量为12.626 g/L与ZY03的胞外多糖产量为11.5148 mg/L在分别以2%蔗糖+0.2%蛋白胨、2%蔗糖+0.3%蛋白胨组合试验中值最大；不同碳、氮源对荷叶离褶伞菌丝体生物量及胞外多糖产量的影响不呈正相关，菌株ZY02、ZY03分别是各指标下的适宜发酵菌株。
- Abstract:
- Taking Lyophyllum decastes as material,the effects of different C and N-sources on mycelial biomass and exopolysaccharide production by five strains of Lyophyllum decastes were studied by single factor liquid fermentation experiment.The aim was to screen the optimum C-sources,N-sources and suitable fermenting strains for Lyophyllum decastes liquid culture,which was the basis of exploring its main factory fermentation.The results showed that the best C-source of strains ZY01,ZY02 and ZY03 was sucrose,the best N-source was peptone,followed by offals,urea.The mycelium biomass of strains ZY04 and ZY05 was most with glucose and peptone as optimum C and N-sources,sucrose was the best C-sources for its exopolysaccharide yield,the best N-sources was respectively urea,peptone.The mycelium biomass (12.626 g/L) and exopolysaccharide production (11.5148 mg/L) of strain ZY02 was most with combination of 2% sucrose+0.2% peptone and 2% sucrose+0.3%peptone,respectively.Therefore,the effect of different C and N-sources on mycelial biomass and exopolysaccharide production was not a positive correlation,strain ZY02 and ZY03 was respectively the suitable fermenting strains of two kinds of indicators.
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备注/Memo
第一作者简介：周会明（1984-）,男，硕士，助教，研究方向为大型真菌驯化与遗传。E-mail:632243530@qq.com.