LIU Ya-jie,CHANG Ping,TANG Dong-qin.Effect of Exogenous Sugars on Active Oxygen Balance of Cut Freesia Flowers[J].Northern Horticulture,2014,38(09):140-147.
外源糖对香雪兰切花活性氧平衡的影响
- Title:
- Effect of Exogenous Sugars on Active Oxygen Balance of Cut Freesia Flowers
- 文章编号:
- 1001-0009(2014)09-0140-08
- 关键词:
- 上海交通大学 农业与生物学院; 上海 200240
- Keywords:
- Freesia hybrid; vase holding; petals aging; active oxygen balance
- 分类号:
- S 682.2+9
- 文献标志码:
- A
- 摘要:
- 以香雪兰园艺品种‘上农金皇后’为试材，将其鲜切花瓶插于6%葡萄糖溶液和蒸馏水（对照）中，研究其瓶插期间花瓣抗氧化酶活性和膜脂过氧化水平的变化。结果表明：整个花序的观赏期为10 d左右，而单朵小花瓶插寿命却不足1周。花瓣吸水量在瓶插期间发生明显变化，表现为在瓶插前期下降，7 d后有所回升。对照中可溶性蛋白质含量呈逐渐下降趋势，添加了6%葡萄糖溶液的处理在瓶插第4天出现1个峰值后其含量才逐渐下降，直到瓶插末期。超氧化物歧化酶（SOD）活性和过氧化氢酶（CAT）活性在瓶插期间变化趋势一致，均为先上升后下降的过程。过氧化物酶（POD）活性在整个瓶插期间持续上升，在花序中不同部位活性存在差异，基部最高，上部最低。花瓣中丙二醛（MDA）含量随瓶插时间增加而上升，同一瓶插期基部小花MDA含量高于中上部小花。在瓶插前2 d，超氧自由基（O2）含量明显上升，之后有一短暂下降过程，第4天后又逐渐上升；6%葡萄糖溶液处理和对照变化趋势一致，但在瓶插不同时期及花序不同位置上，O2含量有所差别。综合各因素表明，失水、抗氧化酶活性下降、自由基的积累以及膜脂过氧化水平的增加可能是香雪兰切花衰老的主要原因。
- Abstract:
- Taking cut flowers of Freesia hybrid ‘Shangnong Jinhuanghou’ as material,being immersed in 6% glucose solution (treatment group) and distilled water (control),respectively,the changes of antioxidant enzyme activity and membrane lipid peroxidation level in petals during vase holding were studied.The results showed that the vase life of a whole inflorescence was about 10 d,while an individual floret was less than one week.Water absorption content in petals changed obviously during the vase holding,with a decrease in the early stage and picking up after 7 d.Soluble protein content in control showed a trend of gradual decline, while a peak was observed at 4 d in treatment group followed by a constant decrease up to the end of vase holding.Superoxide dismutase (SOD) and catalase (CAT) activities had a consistent trend,rising early and then decreasing during the vase holding.However,peroxidase (POD) activity presented a constant increase trend.Meanwhile,a significant difference in POD activity was observed at different position of freesia at the same vase time,respectively.The content of Malondialdehyde (MDA) accumulated gradually during vase holding,and the higher MDA content was observed in base floret than that in middle floret and upper floret at the same vase time,respectively.The content of super oxygen free radicals (O2) increased obviously at 2 d followed by a drop at 4 d,and then increased up to the end of vase holding.Change trend of treatment group was consist with control,but O2 content was varied in different periods and disparate positions of the inflorescence.The results revealed that the water loss,decreased activity of antioxidant enzymes,the accumulation of O2 and the rising of membrane lipid peroxidation level were considered to be the main causes of senescence of cut Freesia flowers.
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备注/Memo
第一作者简介：刘亚杰（1987-），女，硕士，研究方向为园林植物与观赏园艺。E-mail：814482254@sjtu.edu.cn.