ZHANG Jian-ying,MAO Xiang-hong,ZHANG Ying-ying.Study on the Genetic Diversity of Walnut Germplasm Resources Based on RAMP Marker[J].Northern Horticulture,2014,38(09):106-108.
核桃种质资源遗传多样性的RAMP标记分析
- Title:
- Study on the Genetic Diversity of Walnut Germplasm Resources Based on RAMP Marker
- 文章编号:
- 1001-0009（2014）09-0106-03
- Keywords:
- walnut; RAMP markers; germplasm resources; genetic diversity; cluster analysis
- 分类号:
- S 664.1
- 文献标志码:
- A
- 摘要:
- 以核桃嫩叶为试材，对34份核桃种质资源进行了RAMP技术分析研究，以期建立基因组DNA的有效提取方法。结果表明：利用11对引物共扩增出185个条带，其中多态性条带142个，多态性条带比率62.5%～93.3%;34份核桃样品间的相异系数0.0274～0.4848。表明34份核桃种质资源具有较丰富的遗传多样性，用 RAMP 进行核桃资源的DNA 指纹分析可行；核桃的遗传基础具有一定的地域特征，“石门核桃”种群在遗传关系上是相对独立的栽培群体。
- Abstract:
- Taking walnut as material,34 walnut germplasm resources were analyzed by RAMP technology,in order to establish genomic DNA extraction method.The results showed that eleven primers amplificated 185 DNA bands totally,142 bands among which were polymorphic.The rate of polymorphic bands were 62.5%~93.3%.The dissimilarity coefficient among samples were 0.0274 to 0.4848,which indacted that 34 walnut germplasm had abundant genetic diversity.RAMP wass a feasible approach for DNA fingerprint of walnut germplasm resources.The genetic basis of walnut had a certain regional features,and ‘Shimen’ walnut was a relatively independent cultivated population in genetic relation.
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备注/Memo
第一作者简介：张建英（1970-），女，本科，高级工程师，现主要从事经济林育种与栽培工作。E-mail：lkyjjl@163.com.