[1]武玉环,鲁建斌,尉文彬,等.黄瓜枯萎病菌的分离纯化及药剂筛选[J].北方园艺,2014,38(03):111-114.
　WU Yu-huan,LU Jian-bin,YU Wen-bin,et al.Isolation and Fungicide Screening of Cucumber Fusarium Wilt[J].Northern Horticulture,2014,38(03):111-114.

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黄瓜枯萎病菌的分离纯化及药剂筛选

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第38卷期数: 2014年03期页码: 111-114 栏目: 出版日期: 2014-02-10

Title:: Isolation and Fungicide Screening of Cucumber Fusarium Wilt

文章编号:: 1001-0009(2014)03-0111-04

作者:: 武玉环¹; 鲁建斌²; 尉文彬³; 武亚明¹; 张红杰¹; 1.河北北方学院，河北张家口 075000；
2.张家口市农业信息网站，河北张家口 075000；
3.张家口市农业科学院，河北张家口 075000

Author(s):: WU Yu-huan¹; LU Jian-bin²; YU Wen-bin³; WU Ya-ming¹; ZHANG Hong-jie¹; 1.Hebei North University，Zhangjiakou，Hebei 075000；
2.Agricultural Information Websites of Zhangjiakou，Zhangjiakou，Hebei 075000；
.Zhangjiakou Academy of Agricultural Sciences，Zhangjiakou，Hebei 075000

关键词:: 黄瓜; 枯萎病; 分离; 药剂筛选

Keywords:: cucumber; Fusarium wilt; isolation; fungicide screening

分类号:: S 642.2

文献标志码:: A

摘要:: 以感黄瓜枯萎病的病株为试材，采用组织分离法对其病原菌进行分离和形态观察；采用生长速率法，研究测定了12种化学药剂对黄瓜枯萎病菌的毒力；并通过盆栽试验测定了室内抑制效果较好的7种化学药剂对黄瓜枯萎病的防治效果。结果表明：经鉴定，感黄瓜枯萎病病菌为尖镰孢菌（Fusarium oxysporum）；抑菌效果最好的是36%噁霉?福美双可湿性粉剂1 000倍液，抑制率为93.92%，其次是10%苯醚甲环唑水分散粒剂1 500倍液、62.5%精甲?咯菌腈悬浮剂1 500倍液和99%恶霉灵粉剂3 000倍液，抑制率分别为85.48%、81.20%和80.45%；80%嘧霉胺水分散粒剂1 500倍液、99%恶霉灵粉剂3 000倍液和32.5%苯甲?嘧菌酯悬浮剂1 500倍液对黄瓜枯萎病的防治效果为79.48%~93.61%，可作为土壤处理剂防治黄瓜枯萎病，而且对黄瓜安全无害，具有较好的应用前景。

Abstract:: Taking cucumber disease plants of fusarium wilt as material，the pathogen was isolated through tissue isolation and pathogen morphological observation of cucumber was done，and toxicity tests of twelve fungicides to Fusarium oxysporum were carried out using inhibiting growth rate in laboratory，and the effect of seven different kinds of chemical agents on control cucumber fusarium wilt was investigated by potted plant trial.The results showed that the isolate was identified as Fusarium oxysporum.The effect of 36% evil mildew ? thiram WP 1 000 times liquid was the best with control effect of 93.92%，followed by 10% difenoconazole WG 1 500 times liquid，62.5% fine fludioxonil FS 1 500 times liquid and 99% hymexazol powder 3 000 times liquid with control effect 85.48%，81.20% and 80.45% respectively.80% pyrimethanil WDG，99% hymexazol powder and 32.5% benzoyl?azoxystrobin SC were good fungicides for control cucumber fusarium wilt，and the control effect was between 79.48%~93.61%.These fungicides could be used as a soil treatment agent on cucumber fusarium wilt，and they were safe to cucumber，so they should be widely exploited and extended in the future.

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备注/Memo

第一作者简介：武玉环（1981-），女，河北衡水人，硕士，讲师，研究方向为植物病理学。

基金项目：张家口市科技攻关资助项目（12110034C-3）。

更新日期/Last Update: 2014-08-22