HOU Jiang-tao,GAO Qi-ming,KARIM·Ali.Effect of Salt Stress on the Content of K+,Na+,Cl- and Variations of Ultra Structures in Roots of Two Species of Amygdalus communis L.[J].Northern Horticulture,2014,38(23):1-5.
NaCl胁迫对两种扁桃幼苗根系K+、Na+、Cl-含量和超微结构的影响
- Title:
- Effect of Salt Stress on the Content of K+,Na+,Cl- and Variations of Ultra Structures in Roots of Two Species of Amygdalus communis L.
- 文章编号:
- 1001-0009(2014)23-0001-05
- Keywords:
- almond; salt stress; contributions of inorganic ions; ulstrastructure
- 分类号:
- S 662.906+.2
- 文献标志码:
- A
- 摘要:
- 以石头扁桃和桃扁桃为材料，采用盆栽方法，研究了盐胁迫对2种扁桃幼苗根部K+、Na+和Cl-含量的影响和超微结构的变化，以揭示其耐盐性差异机制。结果表明：NaCl胁迫下，2种扁桃根部Na+、Cl-含量均随NaCl浓度的升高和胁迫时间延长而增加，桃扁桃根部Na+、Cl-相对增长量大于石头扁桃；K+含量均随盐浓度的增加而降低，桃扁桃根中K+含量大于石头扁桃。2种扁桃根中Na+/K+随盐浓度的增加而升高，在高盐浓度胁迫下，桃扁桃根中Na+/K+值较石头扁桃低。盐胁迫后，2种扁桃根细胞核膜不清晰，细胞中均出现很多大的液泡，根细胞导管中出现大量淀粉粒。扁桃根细胞中Na+、Cl-含量明显增加，根部细胞核膜发生解离，导管扭曲严重，说明离子毒害起到破环作用，但是没有发生明显的质壁分离，不能明确表明渗透胁迫的同时发生。桃扁桃根部根组织维持较低的Na+/K+值，其受危害的程度小于石头扁桃，表现出较强的抗盐能力。
- Abstract:
- Taking Amygdalus communis L.and A.commans var. persicoides as test materials.The two species were grew in pots to study the effect of salt stress on content of K+,Na+,Cl- and on variations of ultra structures in roots.The objective was to show the differences of salt tolerance between the two species.The results showed that,the content of Na+ and Cl- in roots of the two species were increased as the concentration of NaCl increased with time longer,and the growth of the content of Na+ and Cl- in roots of Amygdalus communis L. was higher than that of A.commans var. persicoides.As the concentration of NaCl increased,the content of K+ in roots of the two species was decreased,the content of K+ in A.commans var.persicoides was higher than that of Amygdalus communis L..The ratio of Na+ to K+ in both two species were increased as the concentration of NaCl increased.The ratio of Na+ to K+ in roots was lower in A.commans var. persicoides than that in Amygdalus communis L.under salt stress.Under salt stress,the nuclear membrane in roots of two species was not clear,and many big vacuoles appeared in cells while many starch grains appeared in ducts of roots.The content of Na+ and Cl- in roots of the two species was increased,the nuclear membrane in roots of two species was not clear and catheter distorted seriously,which indicated ion breaking,but there was not noticeable plasmolysis and didn’t clearly indicate osmotic stress.The ratio of Na+ to K+ was lower in root organizations of A.commans var. persicoides than that in Amygdalus communis L.,which showed no serious damage and indicated strong salt-resistant ability.
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备注/Memo
第一作者简介：侯江涛（1980-），女，硕士，讲师，现主要从事园艺作物抗逆等研究工作。E-mail：250647155@qq.com.