WANG Miao-miao,MA Xiao-yue,ZHANG Xue-ying,et al.Study on Rapid-Micropropagation Technique by Shoot Tip Culture of New Apple Dwarf Rootstock Strain ‘Short Stock No.6’[J].Northern Horticulture,2014,38(22):102-104.
苹果矮化砧木新品系“矮砧6号”茎尖组培快繁研究
- Title:
- Study on Rapid-Micropropagation Technique by Shoot Tip Culture of New Apple Dwarf Rootstock Strain ‘Short Stock No.6’
- 文章编号:
- 1001-0009（2014）22-0102-03
- 分类号:
- S 661.103.6
- 文献标志码:
- A
- 摘要:
- 以苹果矮化砧木新品系“矮砧6号”茎尖为外植体，研究了培养基中不同植物生长调节剂配比对芽苗增殖和生根的影响，以筛选出适合“矮砧6号”茎尖组培快繁的培养基配方。结果表明：苹果矮化砧木“矮砧6号”茎尖用0.1% HgCl2杀菌处理的适宜时间为8 min；以MS为基本培养基，附加蔗糖30 g/L、琼脂6 g/L，适宜增殖培养的植物生长调节剂配比为6-BA 1.0 mg/L+NAA 0.05~0.10 mg/L，增殖系数可达到每4周4.53倍；有利于生根的继代培养基的植物生长调节剂配比为6-BA 1.0 mg/L+NAA 0.1 mg/L，适宜组培苗生根的植物生长调节剂为IAA 1.0 mg/L+IBA 0.4 mg/L，平均生根率为90%以上，平均生根数为6条左右，根系发达，移栽后成活率达90%以上。
- Abstract:
- Taking the new strain ‘Short Stock No.6’ of apple dwarf rootstock as materials,the effects of plant growth regulators on multiplication and rooting of seedlings in vitro were researched to selecte the optimal medium.The results showed that the sterilization time of shoot tip using 0.1% HgCl2 was 8 minutes.The basic medium was MS supplemented with sugar 30 g/L and agar 6 g/L,the optimal plant growth regulators in subculture medium were 6-BA 1.0 mg/L+NAA 0.05-0.10 mg/L,and the coefficient of multiplication reached 4.53 times every four weeks.The optimal plant growth regulators of subculture medium that suited rooting were 6-BA 1.0 mg/L+NAA 0.1 mg/L,the optimal plant growth regulators for rooting were IAA 1.0 mg/L+IBA 0.4 mg/L,the rooting rate was more than 90%,and the average number of root was about 6.Then the rooted plantlets were transplanted,the survival rate was more than 90%.
参考文献/References:
[1]韩振海.苹果矮化密植栽培——理论与实践[M].北京:科学出版社,2011.
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备注/Memo
第一作者简介：王淼淼（1989-），女，河北石家庄人，硕士研究生，研究方向为果树生物技术。E-mail:920487374@qq.com.