[1]周晓馥,杨伟新,丁雪,等.月季茎段快繁体系的优化[J].北方园艺,2014,38(22):98-102.
　ZHOU Xiao-fu,YANG Wei-xin,DING Xue,et al.Optimization of Rapid Propagation System of Rose Stems[J].Northern Horticulture,2014,38(22):98-102.
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月季茎段快繁体系的优化

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第38卷 期数: 2014年22期 页码: 98-102 栏目: 出版日期: 2014-11-30
Title:
Optimization of Rapid Propagation System of Rose Stems
文章编号:
1001-0009（2014）22-0098-05
作者:
周晓馥; 杨伟新; 丁雪; 杜丰平; 张鑫; 徐洪伟
吉林师范大学，生物资源与环境信息吉林省高校重点实验室，吉林 四平 136000
Author(s):
ZHOU Xiao-fu; YANG Wei-xin; DING Xue; DU Feng-ping; ZHANG Xin; XU Hong-wei
Key Laboratory for Plant Resources Science and Green Production,Jilin Normal University,Siping,Jilin 136000
关键词:
月季; 外源激素; 活性炭; 离体再生体系
Keywords:
rose; exogenous hormones; active carbon; in vitro regeneration system
分类号:
S 685.120.36
文献标志码:
A
摘要:
以月季无菌苗茎段为试材，以MS为基础培养基，探究细胞分裂素6-BA、TDZ对茎段增殖的影响，TDZ与NAA 2种激素的配比对株高、节间的影响，及活性炭浓度对月季组培苗生根的影响。结果表明：月季单芽茎段在激素浓度为6-BA 4.0 mg/L培养基上增殖数最高，确定MS+6-BA 4.0 mg/L+活性炭0.6 g/L为月季茎段最适芽增殖培养基；当TDZ浓度为1.0 mg/L，NAA浓度为0.05 mg/L时，株高最大达5.02 cm，节间长度最大达到0.40 cm；月季茎段的最适生根培养基为1/2 MS+活性炭1.0 g/L，生根率为92.9%。
Abstract:
Taking aseptic rose stem segments as materials and MS as basal medium,effect of exogenous 6-BA,TDZ on rose stem proliferation,TDZ and NAA on plant height and internode,active carbon concentration on rooting were explored.The results showed that 6-BA 4.0 mg/L had a maximum bud proliferation,so MS+6-BA 4.0 mg/L+active carbon 0.6 g/L was the optical proliferation medium.When TDZ was 1.0 mg/L,NAA was 0.05 mg/L,seedling had the maxium height of 5.02 cm and internode 0.40 cm.The optimum rooting medium was 1/2 MS+active carbon 1.0 g/L with a 92.9 rooting rate.

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备注/Memo

第一作者简介：周晓馥（1964-），女，博士，教授，硕士生导师，研究方向为植物分子生物学。E-mail:sunflower329@163.com.

责任作者：徐洪伟(1964-)，男，博士，教授，硕士生导师，研究方向为植物分子生物学。E-mail：zhouxiaofu@jlnu.edu.cn.
基金项目：国家自然科学基金资助项目（31070224）。
收稿日期：2014-07-17

更新日期/Last Update: 2014-12-12