ZENG Wen-dan,LUO Xing-lu,GUO Ya-jing,et al.Cloning and Sequence Analysis of SSII Gene in Manihot esculenta Crantz[J].Northern Horticulture,2014,38(02):99-103.
木薯可溶性淀粉合成酶基因SSII克隆及生物学分析
- Title:
- Cloning and Sequence Analysis of SSII Gene in Manihot esculenta Crantz
- 文章编号:
- 1001-0009(2014)02-0099-05
- 关键词:
- 木薯; 可溶性淀粉合成酶基因SSII; 克隆; 生物学分析
- Keywords:
- Taking Manihot esculenta Crantz cultivar SC124 as material; by RT-PCR technology; the whole sequece of SSII gene was firstly obtained.The results showed that the gene contained an 2 256 bp open reading frame (ORF) of SSII and encoding 751 amino acids.Comparaing to the published sequence(EF667961. 1); the homology of nucleotide sequence could shares 99%.However; the homology was low comaraing with other plants; about 77%~84%.The analysis of SSII polymorphism showed that there were alleles and paralogous gene in Manihot esculenta Crantz gene groups.
- 分类号:
- S 889+.5
- 文献标志码:
- A
- 摘要:
- 以木薯品种SC124为试材，采用RT-PCR的方法，克隆了木薯可溶性淀粉合成酶基因SSII的全长，并对其进行生物学分析。结果表明：克隆的基因全长包含完整的开放阅读框2 256 bp，编码751个氨基酸，通过核苷酸序列同源性分析，该cDNA与基因库中登录的木薯SSII（登录号为：EF667961. 1）序列同源性达到99%，但与其它植物的同源性较低，为77%～84%。对木薯SSII多态性分析可知，在木薯基因组内存在等位基因及旁系同源基因。
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相似文献/References:
[1]曾文丹,罗兴录,袁圣勇,等.不同方法提取木薯茎总RNA效果的比较研究[J].北方园艺,2013,37(08):103.
ZENG Wen-dan,LUO Xing-lu,YUAN Sheng-yong,et al.Comparative Study on Different Extraction Methods of Total RNA from Stem of Manihot esculenta Crantz[J].Northern Horticulture,2013,37(02):103.
备注/Memo
第一作者简介：曾文丹（1982-），女，湖北随州人，硕士，研究实习员，现主要从事木薯遗传育种等研究工作。E-mail:zengwendan0700208@163.com.