LI Wen-lu,JIANG Shou-yang,ZHANG Nan,et al.Establishment of the Efficient Regeneration System of Cucumber[J].Northern Horticulture,2014,38(20):96-100.
黄瓜高效再生体系的建立
- Title:
- Establishment of the Efficient Regeneration System of Cucumber
- 文章编号:
- 1001-0009（2014）20-0096-05
- Keywords:
- cucumber; cotyledon node; adventitious buds; regeneration system
- 分类号:
- S 642.2
- 文献标志码:
- A
- 摘要:
- 以黄瓜20个栽培品种的子叶、子叶节和下胚轴为试材，进行发芽、分化和诱导分化不定芽试验，建立最适于黄瓜品种的再生体系。结果表明：供试的20个黄瓜品种中，“农家乐”、“神农春四F1”2个品种的发芽情况显著优于其它品种，发芽率分别为66.7%和83.3%；在MS+0.5 mg/mL 6-BA培养基中，20个黄瓜品种不同类型外植体的分化效率以子叶节最佳；“农家乐”、“神农春四F1”使用MS+0.5 mg/L 6-BA+1.0 mg/L AgNO3培养基，芽的增殖系数最高，诱导率分别为50.0%、56.3％。该试验根据不同品种的发芽率以及诱导愈伤组织和芽的分化，建立了黄瓜快速、高频再生体系，为进一步研究黄瓜的遗传转化和基因工程育种提供参考依据。
- Abstract:
- Taking cotyledons,cotyledon node and hypocotyl of 20 kinds of cucumber cultivated cultivars as material,the germination rate,callus and induced differentiation of adventitious buds were tested,the most suitable for cucumber varieties of regeneration system was established.The results showed that the highest germination rate were 66.7% and 83.3% for ‘Nongjiale’and ‘Shennongchunsi F1’ cucumber;the cotyledon node germination rate was the best in the medium of MS+0.5 mg/mL 6-BA for different type explants；‘Nongjiale’ and ‘Shennongchunsi F1’ cucumber in the medium of MS+0.5 mg/L 6-BA+1.0 mg/L AgNO3 got the highest bud value-added factor and the induction rate were 50.0% and 56.3%.According to the germination rate,callus induction and bud differentiation in different varieties,the fast and high frequency regeneration system in cucumber was established.The study would provide the technological basis for the gene engineering of cucumber.
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备注/Memo
第一作者简介：李文璐（1988-）,女，河北邯郸人，硕士研究生，研究方向为蔬菜遗传育种与生物技术。E-mail:wenlujiayou@126.com.
责任作者：杨正安（1974-）,男，云南建水人，博士，副教授，现主要从事蔬菜生物学及生物技术等的教学与科研工作。E-mail：dyangza@aliyun.com.
基金项目：国家自然科学基金资助项目（30972012；31260481）；云南省自然科学基金资助项目（2011FB049）。
收稿日期：2014-05-14