WANG Yang,DONG Ran,GU De-feng,et al.Study on Sporophytic Induction Technology of Phyllitis japonica[J].Northern Horticulture,2014,38(19):93-98.
东北对开蕨孢子体诱导技术的研究
- Title:
- Study on Sporophytic Induction Technology of Phyllitis japonica
- 文章编号:
- 1001-0009（2014）19-0093-06
- Keywords:
- Phyllitis japonica; sporophytic induction; spore multiply
- 分类号:
- S 682.35
- 文献标志码:
- A
- 摘要:
- 以东北对开蕨孢子为试材，采用无菌培养及常规繁殖的方法，研究了常规条件和无菌条件对孢子体诱导的影响。结果表明：孢子最佳萌发培养基为1/4MS无糖培养基；最佳增殖培养基为MS+15 g/L蔗糖；最佳孢子体无菌诱导培养基为MS培养基；最适宜的常规诱导条件是以草炭为最适宜基质，温度20~30℃，空气湿度95%。
- Abstract:
- Taking spore of Phyllitis japonica as experimental material，the effect of general conditions and sterile conditions on the sporophyte induce of Phyllitis japonica were studied by using normal condition and isolated culture of breeding methods.The results showed that the best germination medium was 1/4MS sugar-free culture medium.The best multiplying culture was MS+15 g/L sucrose.The best sporophyte inductive medium was MS.The best conventional induction conditions was turf,the temperature was 20~30℃ and the air humidity was 95%.
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相似文献/References:
[1]李金鹏,王克凤,芦廷峰,等.东北对开蕨植物研究进展[J].北方园艺,2021,(20):138.[doi:10.11937/bfyy.20210546]
LI Jinpeng,WANG Kefeng,LU Tingfeng,et al.Research Progress on Phyllitis japonica Kom．[J].Northern Horticulture,2021,(19):138.[doi:10.11937/bfyy.20210546]
备注/Memo
第一作者简介：王阳（1989-），女，硕士研究生，研究方向为野生植物引种驯化。E-mail：young0608@qq.com.
责任作者：董然（1966-），女，吉林长春人，博士，教授，现主要从事野生植物引种驯化等研究工作。E-mail:dongr999@163.com.
基金项目：国家“十二五”科技支撑计划资助项目（2012BAD22B0401）；吉林省科技发展计划资助项目（20110262）。
收稿日期：2014-06-10