ZHOU Xing-chen,LI Peng,GU Pei-wen.System Optimization of TLC and HPLC Simultaneous Determination of Sophoridine and Matrine in Total Alkaloid of Sophora alopecuroides L.[J].Northern Horticulture,2014,38(18):41-43.
TLC和HPLC法同时测定苦豆子总碱中槐定碱和苦参碱的体系优化
- Title:
- System Optimization of TLC and HPLC Simultaneous Determination of Sophoridine and Matrine in Total Alkaloid of Sophora alopecuroides L.
- 文章编号:
- 1001-0009(2014)18-0041-03
- Keywords:
- Sophora alopecuroides L.; alkaloid; TLC; HPLC
- 分类号:
- Q 946.8
- 文献标志码:
- B
- 摘要:
- 采用薄层层析法（TLC），展开剂为氯仿-甲醇-氨水=8∶2∶0.1；同时采用高效液相色谱法（HPLC），色谱柱为Ultra IITM C18(5 μm×250 mm×4.6 mm)、流动相为0.01 mol/L磷酸盐缓冲液-甲醇(45∶55)、检测波长为216 nm、流速为1.0 mL/min、柱温为30℃、进样量为10 μL；建立TLC和HPLC同时分离测定苦豆子生物碱体系的优化方法。结果表明：槐定碱和苦参碱在200～900 μg/mL浓度范围内呈良好的线性关系，R2分别为0.9994和0.9996。平均回收率(n=9)分别为99.47%和99.82%；试验表明可通过TLC和HPLC法同时测定苦豆子总碱中槐定碱和苦参碱的含量，并可用该方法进行苦豆子药材及含苦豆子药用成分的质量控制。
- Abstract:
- Thin layer chromatography(TLC) was adopted，developing solvent was chloroform-methanol-ammonia=8∶2∶0.1;meanwhile，the chromatographic column of high performance liquid chromatography(HPLC) included Ultra IITM C18(5 μm×250 mm×4.6 mm) and mobile phase consisting of a mixture of 0.01 mol/L phosphate buffer-methanol (45∶55)，the detection wavelength was 216 nm，the flow rate was 1.0 mL/min;to establish the system optimization of TLC and HPLC methods for simultaneous separation and determination of alkaloids of Sophora alopecuroides L.The results showed that，the method had a good linearity and correlation coefficient:the sophoridine and matrine were 200～900 μg/mL.The mean recoveries (n=9) were 99.47% and 99.82% respectively.The experiment showed that，this method was simple，rapid and accurate assay for the determination of sophoridine and matrine in total alkaloid of Sophora alopecuroides L.，and suitable for quality control in the production of Sophora alopecuroides L.
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备注/Memo
第一作者简介：周星辰（1988-），男，辽宁丹东人，硕士研究生，研究方向为生物防治与微生物资源利用。
责任作者：顾沛雯（1969-），女，宁夏银川人，博士，教授，现主要从事植物病理学等教学与科研工作。E-mail:gupeiwen2013@126.com.
基金项目：国家自然科学基金资助项目（31260452）。收
稿日期：2014-04-22