[1]赵利,王玉珍,吕恃衡,等.木奈叶片β-羟脂酰-CoA脱水酶(HCD) 基因全长cDNA的分离与表达研究[J].北方园艺,2014,38(16):100-104,105.
　ZHAO Li,WANG Yu-zhen,LV Shi-heng,et al.Study on Isolation of Full-length cDNA of HCD from Leaf of Prunus salicina and Its Expression[J].Northern Horticulture,2014,38(16):100-104,105.
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木奈叶片β-羟脂酰-CoA脱水酶(HCD) 基因全长cDNA的分离与表达研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第38卷 期数: 2014年16期 页码: 100-104,105 栏目: 出版日期: 2014-08-30
Title:
Study on Isolation of Full-length cDNA of HCD from Leaf of Prunus salicina and Its Expression
文章编号:
1001-0009(2014)16-0100-06
作者:
赵利1; 2; 王玉珍1; 2; 吕恃衡1; 潘东明1; 2; 姜翠翠3; 陈桂信1; 2
1.福建农林大学 园艺学院，福建 福州 350002；
2.福建农林大学 园艺产品贮运保鲜研究所，福建 福州 350002；
3.福建省农业科学院 果树研究所，福建 福州 350013
Author(s):
ZHAO Li1; 2; WANG Yu-zhen1; 2; LV Shi-heng1; PAN Dong-ming1; 2; JIANG Cui-cui3; CHEN Gui-xin1; 2
1.College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002;
2.Institute of Storage Science and Technology of Horticultural Products,College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002；
3.Fruit Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013
关键词:
β-羟脂酰-CoA脱水酶; 全长cDNA; 表达分析
Keywords:
Prunus salicina; β-hydroxyacylCoA dehydratase; full-length cDNA; expression analysis
分类号:
Q943.2
文献标志码:
A
摘要:
以木奈（Prunus salicina Lindl.var.cordata J.Y.Zhangetal）5个不同生长发育时期叶片为试材，根据已构建木奈5K.tif,JZ的5个不同生长发育时期叶片的均一化全长cDNA文库，分离得到了一个羟脂酰CoA脱水酶(HCD)基因，命名为PsHCD 。对PsHCD基因生物信息进行分析，同时利用Real-time PCR检测PsHCD基因在叶片中5个不同生长发育时期的表达量。结果表明：该基因的cDNA开放阅读框编码区为第322个核苷酸到第987个核苷酸，编码221个氨基酸残基,5′UTR长度为321 bp，3′UTR长度为156 bp。利用TMHMM server软件分析，该基因编码的蛋白质有4个跨膜区域,PSORT Ⅱ Prediction软件分析推测，亚细胞定位于内质网、微体和溶酶体中。实时荧光定量PCR结果表明，PsHCD的表达量随着叶片生长逐渐升高，在展开叶时达到最高峰，在幼叶到成熟叶时处于一种稳定水平，随后降低，老叶表达量最低。
Abstract:
Taking five different growth period of Prunus salicina’s leaves as material,a clone named PsHCD was separated from cDNA library prepared of five different growth and development periods of leaf in the Prunus salicina.The biological information of PsHCD was analyzed and gene expression levels in five different growth periods of Prunus salicina’s leaves were tested using Real-time PCR.The results showed that the gene cDNA open reading frame coding regions for the 322thnucleotide to 987th,encoding a 221 amino acid proteins,with 321 bp in the 5’UTR and 156 bp in the 3’UTR.Using TMHMM server software to analysis,the gene encoding the protein had four transmembrane domains.PsHCD was localized in endoplasmic reticulum,microbodies and lysosomes by PSORT Ⅱ Prediction software.The results of Real-time PCR showed that the PsHCD gene was gradually increasing with development of leaves,reached to maximum at stage of unfold leaves,was stable during spire to climax leaves,then decreased,reached to minimum at stage of old leaves.

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备注/Memo

第一作者简介：赵利(1989-)，女，硕士研究生，研究方向为果树生物技术。

责任作者：陈桂信（1969-），男，博士，副教授，现主要从事果树生物技术教学与科研工作。E-mail:guixinchen@126.com.
基金项目：国家科技部科技支撑计划资助项目（2007BAD07B01）；福建农林大学创新团队资助项目（cxtd12013）。

更新日期/Last Update: 2014-09-26