WANG Dan,YANG Ai-kuan,LI Guang-hong,et al.The Effect of LED Light Qualities on Physiological and Biochemical Characteristics of the Orchid Hybrid (♀:Cymbidium lowianum;♂:Cymbidium eburneum Lindl) Tissue Culture Plantlets[J].Northern Horticulture,2014,38(16):61-65,66.
LED光质对碧玉兰×独占春组培苗生理生化影响
- Title:
- The Effect of LED Light Qualities on Physiological and Biochemical Characteristics of the Orchid Hybrid (♀:Cymbidium lowianum;♂:Cymbidium eburneum Lindl) Tissue Culture Plantlets
- 文章编号:
- 1001-0009(2014)16-061-06
- 关键词:
- LED光质; 碧玉兰×独占春组培苗; 生理生化
- Keywords:
- light emitting diodes (LEDs); the orchid hybrid (♀：Cymbidium lowianum; ♂：Cymbidium eburneum Lindl)tissue culture plantlets; physiological and biochemical characteristics
- 分类号:
- S682.31
- 文献标志码:
- A
- 摘要:
- 以碧玉兰♀×独占春♂组培苗为试材，研究了LED光源（以荧光灯作为对照）不同光质配比组合对兰花组培苗生理生化指标的影响。结果表明：红蓝绿光质（RBG）培养下的植株，形态指标综合系数较高。红蓝白光质（RBW）促进植株叶绿素a、b和胡萝卜素含量增加；蓝光（B）处理下的植株可溶性糖含量显著增加；白光（W1）和红蓝光质（1RB）培养的植株可溶性蛋白质含量较高。红光（R）处理的植株MDA含量最高；红蓝绿光质（RBG）培养的植株SOD活性最高；白色荧光灯（W2）处理下的植株POD活性最高；红蓝白光质（RBW）处理的植株CAT活性最高。研究表明，红蓝绿组合光质（RBG）培养的碧玉兰♀×独占春♂组培苗各项形态指标综合系数较高；LED复合光质促进兰花叶绿素a、b和胡萝卜素的合成；蓝光（B）有利于兰花可溶性糖的积累；1RB、W1光质能促进兰花蛋白质的合成；红光提高了兰花SOD活性、POD活性、CAT活性，有效地清除细胞中的超氧自由基，缓解对细胞质的伤害。
- Abstract:
- Taking the orchid hybrid (♀:Cymbidium lowianum;♂:Cymbidium eburneum Lindl) tissue culture plantlets as test materials,effects of the different light quality and ratio of light emitting diodes (LEDs) on the physiological and biochemical of plantlets were studied.The results showed that under red-blue-green light,the indexes comprehensive coefficient were higher.Red-blue-white light enhanced the contents of chlorophyll a,b and carotene.Under blue light,content of soluble sugar of the plantlets significantly increased.W1 and 1RB light treatments led to higher contents of soluble protein.Under red light,contents of MDA of the plantlets increased.Red-blue-green light enhanced activities of SOD；under fluorescent lamp (W2),activity of POD increased；red-blue-white light enhanced activities of CAT.And it concluded that RBG light could be used as the most suitable for growth of light quality of the orchid hybrid tissue culture plantlets;LED compound light supported chlorophyll a,b and carotene synthesis；the blue light was in favour of the accumulation of soluble sugar；1RB,W1 compound light supported protein synthesis；the red light had important effect on SOD,POD and CAT of the orchid hybrid,effectively eliminated the free radicals in cells,and helped alleviate the damage on the cytoplasm.
参考文献/References:
［1］Maced A F,Leal Costa M V,Tavareses,et al.The effect of light quality on leaf production and development of in vitro-cultured plants of Alternanthera brasilianaKuntze［J］.Environ Exp Bot,2011,70:43-50.
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备注/Memo
第一作者简介：王丹（1987-），女，吉林人，硕士研究生，研究方向为园林植物资源利用与创新。E-mail：398090529@qq.com.