WANG Li-juan,ZHAO Hui,WANG Yan-cai,et al.Cloning and Tissues Expression Analysis of Soluble Acid Invertase Gene from Lycium barbarum.L.[J].Northern Horticulture,2014,38(01):86-90.
枸杞酸性转化酶基因的克隆及组织表达分析
- Title:
- Cloning and Tissues Expression Analysis of Soluble Acid Invertase Gene from Lycium barbarum.L.
- 文章编号:
- 1001-0009（2014）01-0086-05
- 分类号:
- R 282.4
- 文献标志码:
- A
- 摘要:
- 以“宁杞1号”枸杞为试材，通过RT-PCR及RACE技术进行枸杞酸性转化酶基因的克隆及组织表达分析，并运用 MEGA 5.0 软件对植物转化酶基因进行了系统树分析。结果表明：扩增获得2 193 bp 的枸杞酸性转化酶基因，命名为 LbSAI(GenBank：KC776575)，该基因推导氨基酸序列与马铃薯、番茄、梨等酸性转化酶基因氨基酸序列同源性为 68%～100%；LbSAI?编码的蛋白质属于液泡酸性转化酶；Real-time PCR 表达分析显示,LbSAI基因在枸杞花中表达量最高,在根中表达水平较低。
- Abstract:
- Using Lycium barbarum. L.‘Ningqi No.1’ as material,cloning and tissue expression analysis of SAIgenes of Lycium barbarum.L. was studied,and using MEGA 5.0 software to SAIgenes for phylogenetic tree was analyzed.The results showed that by RT-PCR and RACE methods a SAI gene named as Lb SAI (GenBank number：KC776575) was identified from Lycium barbarumL.the full length cDNA of Lb SAI was 2 193 bp;the amino acid sequences of Lb SAIwere 68%～100% identical to the sequences of potato,tomato,pear etc.The putative protein encoded byLb SAIwas belonged to cell wall invertase.Real-time PCR analysis showed that the Lb SAIgene was the highest expressed in flower,the expression level reached the lowest level in root.
参考文献/References:
[1] 白寿宁.宁夏枸杞研究[M].银川：宁夏人民出版社，1999:29-30． [2] Schaffer A A,Aloni B,Fogelman E.Sucrose metabolism and accumulation in developing fruit ofCucumis[J].Phytochem,1987(26)：1883-1887. [3] 王永章,张大鹏.“红富士”苹果果实蔗糖代谢与酸性转化酶和蔗糖合成酶关系的研究[J].园艺学报,2001,28(3):259-261. [4] Hubbard N L,Parr D M,Huber S C.Sucrose-phosphate synthase and other sucrose metabolizing enzymes in fruit of various species[J].Physiol Plant,1991,82:191-196. [5] Winter H,Huber S C.Regulation of sucrose and regulation of activity of key enzymes[J].Crit Rev Plant Sci,2000,19(1)：31-67. [6] 郑国琦,罗霄,买立芸,等.不同品种宁夏枸杞果实糖积累差异的生理原因[J].农业科学研究,2009,30(1):1-4. [7] Haouazine T N,Tymowska L Z,Takvorian A,et al.Characterization of two members of theArabidopsis thalianagene family,Atbfruct3 and Atbfruct4,coding for vacuolar invertases[J].Gene,1997,197:239-251. [8] Lorenz K,Lienhard S,Sturm A.Structural organization and differential expression of carrot b-fructofuranosidase genes：identification of a gene coding for a flower bud_specific isozyme[J].Plant Mol Biol,1995(28):189-194. [9] Mercier R W,Gogarten J P.A second cell_wall acid invertase gene inArabidopsis thaliana[J].Plant Physiol,1995,107：659-660. [10]Kim J Y,Mahe A,Sylvain G,et al.Characterization of two members of the maize gene family,Incw3 and Incw4,encoding cell_wall invertases[J].Gene,2000,245:89-102. [11]Davies C,Robinson S P.Sugar accumulation in grape berries,cloning of two putative vacuolar invertase cDNAs and their expression in grapeving tissues [J].Plant Physiol,1996,111:275-283. [12]Xu J,Avigne W T,Mccarty D R.A similar dichotomy of sugar modulation and developmental expression affects both paths of sucrose metabolism.Evidence from a maize invertase gene family[J].Plant Cell,1996(8):1202-1220. [13]Sturm A.Molecular characterization and functional analysis of sucrose-cleaving enzymes in carrot (DaucuscarotaL.)[J].J Exp Bot,1996,47:1187-1192. [14]Tymowska-Lalanne Z,Kreis M.Expression of theArabidopsis thalianainvertase gene family[J].Planta,1998b,207：259-265. [15]Ranwala A P,Baird W V,Miller W E.Organ specific localization and molecular properties of three soluble invertase fromLilium longiflorumflower buds[J].Physiologia Plantarum,1998,103：551-559. [16]Lorenz K,Lienhard S,Strum A.Structural organization and differential expression of carrot β-fructofuranosidase genes identification of a gene coding for a flower bud-specific isozyme[J].Plant Molecular Biology,1995,28：189-194. [17]Godt D E,Roitsch T.Regulation and tissue specific distribution of mRNAs for three extracellular invertase isoenzymes of tomato suggests：an important function in establishing and maintaining sink metabolism[J].Plant Physiology,1997,115：273-282.
相似文献/References:
[1]武小靖,杨 晴,张国君,等.土壤含盐量对枸杞生理生化指标的影响[J].北方园艺,2014,38(12):137.
WU Xiao-jing,YANG Qing,ZHANG Guo-jun,et al. Effect of Salinity of Soil on Physio-biochemistry Indexes of Lycium barbarum[J].Northern Horticulture,2014,38(01):137.
[2]王亚军,安巍,罗青,等.丛枝菌根真菌菌剂扩繁及菌根化枸杞育苗技术研究[J].北方园艺,2014,38(05):139.
Research on Enlargment Reproducing of AMF Microbial Inoculum and Growing Seedling Technology of Wolfberry With Mycorrhization.WANG Ya-jun1,2,AN Wei1，LUO Qing1，MA Ping3,SHI Zhi-gang1，ZHAO Jian-hua1[J].Northern Horticulture,2014,38(01):139.
[3]樊光辉.“宁杞1号”枸杞辐射诱变育种初报[J].北方园艺,2014,38(02):149.
FAN Guang-hui.Preliminary Report on ‘Ningqi No. 1’ Lycium barbarum Radiation Mutation Breeding[J].Northern Horticulture,2014,38(01):149.
[4]魏雯雯,班兆军,冯建华,等.热处理对‘宁杞4号’鲜食枸杞果实冷藏品质的影响[J].北方园艺,2013,37(10):124.
WEI Wen-wen,BAN Zhao-jun,FENG Jian-hua,et al.Effects of Heat Treatment on Quality of Lycium barbarum Druit During Storage[J].Northern Horticulture,2013,37(01):124.
[5]刘长江,匡 明.枸杞钙果复合运动饮料的研制[J].北方园艺,2013,37(17):129.
LIU Chang-jiang,KUANG Ming.Manufacture of Medlar-calcium Fruit Compound Health Beverage[J].Northern Horticulture,2013,37(01):129.
[6]王忠忠,马茜,慕金凤,等.宁夏枸杞果实发育过程中黄酮类化合物含量的变化研究[J].北方园艺,2013,37(11):152.
WANG Zhong-zhong,MA Qian,MU Jin-feng,et al.Changes of Flavonoid Compounds Content During the Fruit Development of Lycium barbarum L.[J].Northern Horticulture,2013,37(01):152.
[7]鲍瑞,周筠,康建宏,等.不同有机肥对枸杞活性成分的影响[J].北方园艺,2013,37(05):174.
BAO Rui,ZHOU Yun,KANG Jian-hong,et al.Effect of Different Organic Fertilizer Treatments on Active Ingredient of Lycium barbarum L.[J].Northern Horticulture,2013,37(01):174.
[8]郑国琴.,柳金凤.宁夏枸杞叶片组培快繁技术研究[J].北方园艺,2012,36(01):122.
ZHENG Guo-qin.,LIU Jin-feng.Study on Rapid Propagation by Leaf Tissue Culture of the Lycium barbarum L.[J].Northern Horticulture,2012,36(01):122.
[9]何 军,李晓莺,焦恩宁,等.三个枸杞品种花粉直感效应研究[J].北方园艺,2013,37(09):178.
HE Jun,LI Xiao-ying,JIAO En-ning,et al.Study on Pollen Xenia Effect of Three Wolfberry Cultivars[J].Northern Horticulture,2013,37(01):178.
[10]王丽娟,石晶,王彦才.枸杞蔗糖合成酶基因cDNA分离及表达分析[J].北方园艺,2013,37(21):105.
WANG Li-juan,SHI Jing,WANG Yan-cai.Isolation and Expression Analysis of Sucrose Synthase Gene cDNA from Lycium barbarum L.[J].Northern Horticulture,2013,37(01):105.
备注/Memo
第一作者简介：王丽娟(1974-),女，博士，副教授，现主要从事植物基因工程等研究工作。E-mail：Lijuanwang279@hotmail.com.