[1]闫京艳,唐道城,巨秀婷,等.焦锑酸钾沉淀法在百合花柱钙分布研究中的应用[J].北方园艺,2014,38(01):78-80.
　YAN Jing-yan,TANG Dao-cheng,JU Xiu-ting,et al.Application of Potassium Pyroantimonate Precipitation on Calcium Distribution in the Style of Lily[J].Northern Horticulture,2014,38(01):78-80.
点击复制

焦锑酸钾沉淀法在百合花柱钙分布研究中的应用

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第38卷 期数: 2014年01期 页码: 78-80 栏目: 出版日期: 2014-01-10
Title:
Application of Potassium Pyroantimonate Precipitation on Calcium Distribution in the Style of Lily
文章编号:
1001-0009（2014）01-0078-03
作者:
闫京艳; 唐道城; 巨秀婷; 张永玉; 马巧玲
青海大学 高原花卉研究中心，青海 西宁 810016
Author(s):
YAN Jing-yan; TANG Dao-cheng; JU Xiu-ting; ZHANG Yong-yu; MA Qiao-ling
Plateau Flower Research Center,Qinghai University,Xining,Qinghai 810016
关键词:
百合; 钙分布; 焦锑酸钾沉淀法
Keywords:
lily; calcium distribution; potassium pyroantimonate precipitation
分类号:
Q 949.71+8
文献标志码:
A
摘要:
以百合为试材，研究了焦锑酸钾沉淀法用于定位百合花柱钙分布的条件和效果及固定液的pH值对固定效果的影响。结果表明：焦锑酸钾沉淀法准确稳定，样品经含焦锑酸钾的固定液处理后钙沉淀颗粒非常明显，易于识别；而固定液中未添加焦锑酸钾的对照中则不存在黑色钙沉淀颗粒；固定液pH为7.0~7.6时细胞结构清晰度较pH为6.8好,该范围内不同的pH值对固定效果的影响不是很明显。
Abstract:
Using lily as material,the conditions and the effects of potassium pyroantimonate precipitation technique to be used to localize calcium in the style of lily,as well as the influences of fixative solutions with different pH were studied.The results showed that calcium precipitates were so obvious in sample sections dealed with in fixative solutions containing potassium pyroantimonate and could be recognized easily under TEM,while no such calcium precipitate particles could be found in the treatment without potassium pyroantimonate.And the cell structure clarity of samples fixed in solutions with pH differing from 7.0 to 7.6 was relatively better than that fixed in solutions with pH 6.8,although no significant differences were found among this range.

参考文献/References:

[1] Bush D S.Calcium regulation in plant cells and its role in signaling[J].Annu Rev Plant Physiol Plant Mol Biol,1995,46:95-122.

[2] Sanders D,Brownlee C,Harper J F.Communicating with calcium[J].Plant Cell,1999,11：691-706.

[3] 吴志华,郑伟文,庄伟建.钙、钙调蛋白与植物的有性生殖[J].福建农业学报,2002,17（4）:244-247.

[4] 田惠桥,远彤.钙在被子植物受精过程中的作用[J].植物生理学报,2000(26):369-380.

[5] Polito V S.Membrane-associated calcium during pollen grain germination：a microfluorome-tric analysis[J].Protoplasma,1983,117：226-232.

[6] Reiss H D,Herth W.Visualization of Ca2+ gradient in growing pollen tubes of Lilium longiflorumwith chlorotetracycline fluorescence[J].Protoplasma,1978,97:373-377.

[7] Reiss H D,Herth W,Nobiling R.Development of membrane-and calcium-gradients during pollen germination of Lilium longiflorum[J].Planta,1985,163:84-90.

[8] Yu F L,Zhao J,Liang S P,et al.Ultracytochemical localization of calcium in gynoecium and embryo sac of rice[J].Acta Bot Sin,1999,41:125-129.

[9] Zhao J,Yu FL,Liang S P,et al.Changes of calcium distribution in egg cells,zygotes and two-celled proembryos of rice (Oryza sativaL.)[J].Sex Plant Reprod,2002,14:331-337.

[10]谢潮添,杨延红,邱义兰,等.白菜核雄性不育两用系可育和不育花药中Ca2+的分布[J].植物生理与分子生物学学报,2005,31(6):615-624.

[11]尚忠林,马力耕,王学臣,等.细胞外钙调素对百合花粉细胞内钙离子浓度的影响[J].植物学报,2001,43（1）:12-17.

[12]张丰德,王秀玲.现代生物学技术[M].天津:南开大学出版社,2001:52-76.

[13]杨淑娟,宋玉霞,田惠桥,等.枸杞花药发育过程中钙的分布[J].分子细胞生物学报,2006,39(6):516-526.

[14]邓芳,史银连,姚家玲.光敏核不育水稻花药中Ca2+分布及其与花粉育性的相关性研究[J].中国农业科学,2009,42(3):809-815.

[15]彭抒昂.焦锑酸钾沉淀法定位Ca2+在梨生理研究中的应用[J].果树学报,2001,18(3):140-144.

[16]张彦妮,钱灿.12种百合属植物花粉形态扫描电镜观察[J].草业学报,2011,20(5):111-118.

[17]刘选明,何立珍,周朴华,等.百合鳞叶细胞形态发生中胚性细胞的电镜观察[J].湖南农业大学学报,1997,23（1）:30-35.

[18]Wick S M,Hepler P K.Selective localization of intracellular Ca2+ with potassium antimonite[J].J Histochem Cytochem,1982,30:1190-1204.

[19]郭琼杰,孙淑娟.细胞内游离钙离子的测定方法[J].食品与药品,2006,8（12）:25-27.

[20]张国增,李瑞玲,王强.荧光染料在植物细胞Ca2+浓度测定中的应用[J].中国农学通报,2010,26(9):198-201.


相似文献/References:

[1]朱莉,李云飞,王忠义,等.秸秆发酵技术在百合生产中的应用[J].北方园艺,2013,37(04):74.
[2]王丽媛,周厚高.不同营养组合和施肥量对切花百合光合特性及干物质含量的影响[J].北方园艺,2014,38(04):58.
　WANG Li-yuan,ZHOU Hou-gao.Effect of Nutrient Combinations and Fertilizing Amount on the Photosynthetic Characteristics and Dry Matter of Cut-flower Lily[J].Northern Horticulture,2014,38(01):58.
[3]赵兴华,吴海红,杨佳明.百合远缘杂交未成熟子房的离体培养研究[J].北方园艺,2014,38(04):90.
　ZHAO Xing-hua,WU Hai-hong,YANG Jia-ming.Study on Lily Distant Hybridization in vitroCulture of Immature Ovary[J].Northern Horticulture,2014,38(01):90.
[4]吴 超,丁晓瑜,黎 侠,等.东方百合“Siberia”基因组DNA提取方法比较研究[J].北方园艺,2014,38(02):108.
　WU Chao,DING Xiao-yu,LI Xia,et al.Comparative Study on Genomic DNA Extraction Methods From the Oriental Lily ‘Siberia’[J].Northern Horticulture,2014,38(01):108.
[5]周春华,尤超,陈凝华.百合组织培养研究进展[J].北方园艺,2013,37(14):193.
　ZHOU Chun-hua,YOU Chao,CHEN Ning-hua.Research Advances on Tissue Culture of Lilium brownie[J].Northern Horticulture,2013,37(01):193.
[6]夏九成,郑毅,吕会,等.利用百合花器官诱导试管苗快繁技术研究[J].北方园艺,2013,37(01):102.
　XIA Jiu-cheng,ZHENG Yi,LV Hui,et al.The Rapid Propagation of Lily by Inducing Seedling in Test Tube with Floral Organ[J].Northern Horticulture,2013,37(01):102.
[7]时婷婷,张桂玲,温四民,等.百合花粉超低温保存方法研究[J].北方园艺,2013,37(03):64.
　SHI Ting-ting,ZHANG Gui-ling,WEN Si-min,et al.Study on Crypreservation Methods of Lilium spp Pollens[J].Northern Horticulture,2013,37(01):64.
[8]黄海涛,王丹,陈霞.百合耐盐突变体同工酶电泳分析[J].北方园艺,2013,37(03):127.
　HUANG Hai-tao,WANG Dan,CHEN Xia.Isozyme Electrophoresis Analysis of Salt-tolerant Mutant of Lily[J].Northern Horticulture,2013,37(01):127.
[9]王建宇.外施赤霉素对休眠百合生理指标的影响[J].北方园艺,2012,36(02):64.
　WANG Jian-yu.Research on Effects of External GA3on Physiological Index of Dormancy of Lilium siberia[J].Northern Horticulture,2012,36(01):64.
[10]苏頔,方正,李英丽,等.不同氮素形态配比对百合生长发育的影响[J].北方园艺,2012,36(02):67.
　SU Di,FANG Zheng,LI Ying-li,et al.Effect of Different NH4+-N/NO3--N Ratios on the Growth of Lilium[J].Northern Horticulture,2012,36(01):67.

备注/Memo

第一作者简介：闫京艳（1988-），女，硕士研究生，现主要从事园林植物遗传育种等工作。E-mail:yanjingyan@163.com.

责任作者：唐道城（1954-），男，硕士，教授，博士生导师，现主要从事高原花卉研究等工作。E-mail:tangdaocheng6333@163.com.
基金项目：青海省科技厅科研资助项目（2011-H-806）。

更新日期/Last Update: 2014-08-08