SUN Jin-xu.Fermentation Condition Optimization of Antrodia camphorata and the Preliminary Analysis of Triterpene in Antrodia camphorata [J].Northern Horticulture,2013,37(22):156-159.
樟芝真菌深层发酵条件优化及三萜成分初步分析
- Title:
- Fermentation Condition Optimization of Antrodia camphorata and the Preliminary Analysis of Triterpene in Antrodia camphorata
- 文章编号:
- 1001-0009(2013)22-0156-04
- Keywords:
- Antrodia camphorate; triterpene; fermentation
- 分类号:
- S 759. 81
- 文献标志码:
- A
- 摘要:
- 以樟芝真菌为试材，以樟芝三萜类化合物为目的物，利用2 L发酵罐对其深层发酵条件进行优化，10 L发酵罐放大培养，并利用高效液相色谱（HPLC）法对樟芝真菌三萜类化合物种类进行初步分析。结果表明:适宜于樟芝真菌深层发酵产三萜类化合物的条件为：接种方式为菌丝接种,培养温度28℃、培养基初始pH为4、溶氧量控制在20%，此条件下10 L发酵罐放大后，樟芝真菌菌体和三萜类化合物的最大产量分别为3.19 g/L和33.59 mg/L，相对稳定。处理后的樟芝三萜，以齐墩果酸为标准品，经检测共有5种三萜类化合物，其中1种与标准品相近。
- Abstract:
- Taking Antrodia camphorate as material,triterpene compounds of Antrodia camphorate as objective, the fermentation conditions of Antrodia camphorate was optimized by 2 L fermentor and amplified in 10 L fermentor.The triterpene compounds of Antrodia camphorate was analyzed by HPLC.The results showed that the best in oculate way was turf,temperature 28℃,the initial pH 4 and 20% dissolve of oxygen was suitable for Antrodia camphorata fermentation,the maximum yield of Antrodia camphorata mycelia and triterpene was 3.19 g/L and 33.59 mg/L respectively.The oleanolic acid as the standard,the result for the triterpene of Antrodia camphorate being analyzed by HPLC showed that the triterpene of Antrodia camphorate was constituted by five kinds of monomer,one of them was similar to standard.
参考文献/References:
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备注/Memo
作者简介：孙金旭（1975-），男，博士，副教授，现主要从事发酵工程和食品等研究工作。E-mail：wztg8268@163.com.
资助项目：衡水学院重点资助项目（2008031）。
收稿日期：2013-06-24