JIAN Li,YU Dan-feng,QIN Xiao-jun.Comparative Analysis on the Chlorophyll Contents and SPAD Value of Wild Bletilla striata Germplasm Resources[J].Northern Horticulture,2013,37(21):165-167.
野生白芨叶绿素含量与SPAD值的测定与分析
- Title:
- Comparative Analysis on the Chlorophyll Contents and SPAD Value of Wild Bletilla striata Germplasm Resources
- 文章编号:
- 1001-0009(2013)21-0165-03
- Keywords:
- Cymbidium; chlorophyll content; SPAD value; cluster analysis
- 分类号:
- S 567
- 文献标志码:
- A
- 摘要:
- 以20份野生白芨资源为试材，利用SPAD-502叶绿素计和丙酮乙醇混合法对其叶片叶绿素含量进行比较分析。结果表明：不同的白芨种质资源的叶绿素a、叶绿素b和总叶绿素含量以及SPAD值均有所不同，通过相关性分析，发现叶绿素含量和SPAD值之间存在显著的线性相关关系，表明利用叶片SPAD值可以很好的反应整个植株的叶绿素含量水平，且方法简单易行。
- Abstract:
- Taking 20 wild Bletilla striata germplasm resources as materils,the chlorophyll contents of 20 Bletilla striata cultivars were measured and compared by chlorophyll meter SPAD-502 and acetone ethanol mixed methods.The results showed that different cultivars had different chlorophyll a content，chlorophyll b content，total chlorophyll content and SPAD value.The relativity of the chlorophyll content and SPAD value were notability lever.So it concluded that SPAD readings could fully reflect the chlorophyll contents and it had simple and convenient measure without any harmon leaves.
参考文献/References:
[1]陈心启,吉占和,罗毅波.中国野生兰科植物彩色图[M].北京：科学出版社，1999：107. [2]文璨.春兰组培条件和体内多糖的研究[D].成都：四川大学,2005：31. [3]侯大强.春石斛兰离体培养、再生过程生理变化及转基因初步研究[D].杭州：浙江大学，2006. [4]董运斋.大花蕙兰营养特性与施肥的研究[D].北京：北京林业大学，2005：11-13. [5]Loh F，Grabosky J，Bassuk N.191 Use of the minolta SPAD-502 to determine chlorophyll concentration in Ficus benjamina?L.and Populus deltoides?Marsh leaf tissue[J].Hort Science，2000，35(7)：423-424. [6]Han H H，Kim Y K，Lee J Y.Correlation among the nitrogen，chlorophyll (SPAD Value) and photosynthetic reactions on the leaves of one-year-old shoots by training angles in ‘Fuji’ apple trees[J].Hort Science，2004，39(4)：762-763. [7]Babar M A，Reynolds M P，van Ginkel M，et al.Spectral reflectance to estimate genetic variation for in-season biomass，leaf chlorophyll，and canopy temperature in wheat[J].Crop Sic,2006，46(3)：1046-1057. [8]Loh F C W，Grabosky J C，Bassuk N L.Using the SPAD 502 Meter to Assess Chlorophyll and Nitrogen Content of Benjamin Fig and Cottonwood Leaves[J].HortTechnology，2002，12(4)：541-740. [9]Neilsen D，Hogue E J，Neilsen G H，et al.Using SPAD-502 values to assess the nitrogen status of apple trees[J].HortScience，1995，30(3)：508-512. [10]张宪政.植物叶绿素含量测定-丙酮乙醇混合液法[J].辽宁农业科学，1986(3)：26-28.
相似文献/References:
[1]王 薇,宋廷宇,王 艳,等.番茄叶片SPAD值与叶绿素含量的相关性分析[J].北方园艺,2013,37(23):12.
WANG Wei,SONG Ting-yu,WANG Yan,et al.Correlation Analysis on Chlorophyll Content and SPAD Value in Tomato Leaves[J].Northern Horticulture,2013,37(21):12.
[2]刘芳,李怡然,刘峄,等.遮荫对海姆维斯蒂栒子生长及光合特性的影响[J].北方园艺,2014,38(07):52.
LIU Fang,LI Yi-ran,LIU Yi,et al.Effect of Shading on Growth and Photosynthetic Characteristics of Cotoneaster hjelmqvistii[J].Northern Horticulture,2014,38(21):52.
[3]张忠宝,李大成,荆 昕.不同遮荫度和不同栽植密度对独角莲产量的影响[J].北方园艺,2014,38(03):152.
ZHANG Zhong-bao,LI Da-cheng,JING Xin.Effect of Different Shading and Cultivation Density on Yield of Typhonium giganteum [J].Northern Horticulture,2014,38(21):152.
[4]张燕,黎斌,李汝娟,等.白芨种子的无菌萌发过程观察和组培快繁研究[J].北方园艺,2013,37(03):158.
ZHANG Yan,LI Bin,LI Ru-juan,et al.Study on Observation of Aseptic Germination of Bletilla striata Seeds and Rapid Propagation of Bletilla bulbocodioides [J].Northern Horticulture,2013,37(21):158.
[5]潘静,曹兵,万仲武.两种果树叶片SPAD值与叶绿素含量相关性分析[J].北方园艺,2012,36(05):9.
PAN Jing,CAO Bing,WAN Zhong-wu.Relationship Between SPAD Value and Chlorophyll Content in Leaves of Two Fruit Tree Species[J].Northern Horticulture,2012,36(21):9.
[6]王春枝,于小静,齐宝利,等.施肥对南果梨叶片叶绿素含量及光合特性的影响[J].北方园艺,2012,36(14):150.
WANG Chun-zhi,YU Xiao-jing,QI Bao-li,et al.Effect of Fertilize on Chlorophyll Contents and Photosynthetic Characteristics of ‘Nanguo’ Pear[J].Northern Horticulture,2012,36(21):150.
[7]刘伟,丁长春,常征.干旱胁迫下吊石苣苔三种生理指标变化规律研究[J].北方园艺,2014,38(19):65.
LIU Wei,DING Chang-chun,CHANG Zheng.Study on Three Physiological Indexes of Lysionotus pauiflorus Under Drought Stress[J].Northern Horticulture,2014,38(21):65.
[8]杜保伟,方庆,胡月华,等.叶面喷钙对‘3-18’桃叶片性状的影响[J].北方园艺,2014,38(24):21.
DU Bao-wei,FANG Qing,HU Yue-hua,et al.Effect of Spraying CaCl2 on Characters of Peach Strain ‘3-18’ Leaves[J].Northern Horticulture,2014,38(21):21.
[9]钟娟,钟俊荣.水分胁迫对金桔光合特性的影响[J].北方园艺,2015,39(06):26.[doi:10.11937/bfyy.201506007]
ZHONG Juan,ZHONG Jun-rong.Effect of Water Stress on Photosynthetic Characteristics of Fortunella margarita[J].Northern Horticulture,2015,39(21):26.[doi:10.11937/bfyy.201506007]
[10]刘佳学,周祥,胡世方.城市污染对园林景观植物的影响及其对环境的检测[J].北方园艺,2015,39(08):64.[doi:10.11937/bfyy.201508019]
LIU Jia-xue,ZHOU Xiang,HU Shi-fang.Effect of Urban Pollution on the Landscape Plants and Detection of Environment[J].Northern Horticulture,2015,39(21):64.[doi:10.11937/bfyy.201508019]
备注/Memo
第一作者简介：蹇黎(1978-)，女,博士,副教授，研究方向为植物分子育种。