ZHAO Qing,TANG Dao-cheng.Optimization of Microscope Observation Method on Root-tip Chromosomes of Marigold[J].Northern Horticulture,2013,37(20):98-100.
万寿菊根尖染色体观测方法的优化
- Title:
- Optimization of Microscope Observation Method on Root-tip Chromosomes of Marigold
- 文章编号:
- 1001-0009(2013)20-0098-03
- Keywords:
- Tagetes erecta L.; root-tip; chromosome; mitotic split-figure
- 分类号:
- S 682.1+1
- 文献标志码:
- A
- 摘要:
- 以万寿菊‘QU2-2’为试材，从取材时间、根的长度、预处理方法、解离时间、染色时间、压片工具6个方面对根尖染色体制片方法进行了筛选和优化。结果表明：万寿菊根尖染色体观测采用室内催根培养，于上午9：14，剪取根长1.0 cm的根尖，利用低温（0～4℃）水浴进行预处理24 h、1.0 mol/L盐酸解离7 min、染色25 min、结合带橡皮头铅笔压片，可以获得高质量的有丝分裂前中期临时片。
- Abstract:
- Taking the root-tip of Tagetes erecta L.‘QU2-2’ as the experimental material，the material taking time，length of the root，material pretreatment method，dissociating time，dyeing time and squashing tools were screened and optimized.The results showed that using indoor root culture，observed the high-quality images of the chromosomes of root-tips at 9:14，1.0 cm of the root length，the cool water pretreatment (0～4℃)，1.0 mol/L HCl for dissociation in 7 minutes，dyeing in 25 minutes，squashing the slides with a rubber head pencil，could observe clear mitotic prometaphase.
参考文献/References:
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备注/Memo
第一作者简介：赵庆（1989-），女，硕士研究生，现主要从事园林植物遗传育种工作。E-mail:zhaoqing8811@126.com.