CHENG Hua,LI Lin-ling,WANG Shao-bin,et al.The Relationship Between Flower Bud Differentiation and the Changes of Metabolism-related Enzyme Activity in Castanea mollissima ‘Bayuehong’[J].Northern Horticulture,2013,37(20):6-9.
罗田“八月红”板栗花芽分化与相关酶活性的变化
- Title:
- The Relationship Between Flower Bud Differentiation and the Changes of Metabolism-related Enzyme Activity in Castanea mollissima ‘Bayuehong’
- 文章编号:
- 1001-0009(2013)20-0006-04
- Keywords:
- chestnut; flower bud differentiation; enzymatic activity
- 分类号:
- S 664.2
- 文献标志码:
- A
- 摘要:
- 以罗田板栗主栽品种“八月红”为试材,研究分析了板栗花芽分化过程中相关酶活性的变化规律,以期为果树育种工作的顺利进行提供理论基础和技术支撑。结果表明:板栗雌花分化过程中吲哚乙酸(IAA)的大量氧化有利于板栗植株完成从营养生长到生殖生长的转变,从而完成有序花芽分化;过氧化氢酶(CAT)活性随着花芽分化进程的进行不断增强,后期增加趋势转为平缓;过氧化物酶(POD)活性在花芽分化前逐渐上升直到花芽分化期活性达到最高值,之后呈现一段时间的下降趋势;不同分化时期淀粉酶活性降低有利于板栗花芽分化。
- Abstract:
- Taking Castanea mollissima ‘Bayuehong’ as material,the relationship between flower bud differentiation and related enzyme activity in ‘Bayuehong’ were studied,in order to provide theoretical basis and technical support for the work of fruit genetic breeding.The results showed that the IAA content decreased due to the large number of oxidation in the process of chestnut female flower differentiation;the CAT activity in chestnut flower buds was increasing as the flower bud differentiation went on,but the increasing trend became gentle in the last period;the change of peroxidase activity in front of the flower bud differentiation increased gradually until the activity reached to the highest value in the flower bud differentiation stage;the decreasement of amylase activity in the differentiation period was beneficial to the flower bud differentiation.
参考文献/References:
[1]冯学文.实用果树栽培技术[M].天津:天津科学技术出版社,1993. [2]张宇和,王福堂,高新一.板栗[M].北京:中国林业出版社,1989. [3]姜德志,程水源,王燕,等.罗田3个板栗栽培品种主要营养成分分析[J].湖北农业科学,2012,50(23):4882-4884. [4]郭成圆.板栗花芽分化及内源激素变化的研究[D].杨凌:西北农林科技大学,2010. [5]程华,李琳玲,姜德志,等.罗田板栗主栽品种物候期观察[J].北方园艺,2012(23):1-5. [6]徐田军,董志强,兰宏亮,等.低温胁迫下聚糠萘合剂对玉米幼苗光合作用和抗氧化酶活性的影响[J].作物学报,2012,38(2):352-359. [7]张英.竹叶提取物类SOD活性的邻苯三酚法测定[J].食品科学,1997,18(5):47-49. [8]唐云明.柑叶片蔗糖酶的分离纯化及其部分性质的研究[J].植物学报,1995,37(8):594-600. [9]李忠光,龚明.磺胺比色法测定植物组织硝酸还原酶活性的改进[J].植物生理学通讯,2009,45(1):67-68. [10]陈立松,刘星辉.水分胁迫下荔枝叶片过氧化物酶和IAA氧化酶活性的变化[J].武汉植物学研究,2002,20(2):131-136. [11]孙文全.联苯胺比色法测定果树过氧化物酶活性的研究[J].果树学报,1988(3):105108. [12]李雯,邵远志,陈维信.淀粉酶活性测定方法的改进[J].植物生理学通讯,2005,41(5):655-656. [13]陈晓芳,穆鼎,吕英民.低温储藏亚洲百合鳞茎内部淀粉含量和相关酶活性变化[J].栽培生理,2006(2):315-320. [14]吴邦良,春森.树开花结实生理和调控技术[M].上海:科学技术出版社,1995.〓 [15]靳丹丹.碗莲花芽分化形态发育以及相应时期生理生化的动态研究[D].郑州:河南农业大学,2008. [16]孔德政,靳丹丹,孔丽娜,等.碗莲花花芽分化期几种酶活性的变化[J].河南农业科学,2008(4):97-100. [17]郭金丽,玉兰.果梨花芽分化期蛋白质、淀粉代谢的研究[J].蒙古农牧学院学报,1999,20(2):50-53. [18]董合忠,李维江,任桂杰,等.棉花花芽分化过程中IAA含量与过氧化物活性变化趋势的研究[J].棉花学报,1999,11(6):303-305. [19]路苹,郭蕊,于同泉,等.切花百合鳞茎花芽形态分化期碳水化合物代谢变化[J].北京农学院学报,2003,18(4):259-261. [20]张建铭,谈锋,陈京.大花栀子花芽生理分化期内源激素和碳氮比的动态变化[J].西南师范大学学报(自然科学版),1999,24(2):219-224. [21]田华,段美洋,王兰.植物硝酸还原酶功能的研究进展[J].中国农学通报,2009,25(10):96-99. [22]李文平.甘蓝春化过程中相关酶活性及内源激素的变化规律的研究[D].哈尔滨:东北农业大学,2010. [23]张慧君,王学征,高鹏.甜瓜性别分化的研究进展[J].园艺学报,2012,39(9):1773-1780.
相似文献/References:
[1]程 华,李琳玲,袁红慧,等.内源激素含量变化与板栗花芽分化关系研究[J].北方园艺,2013,37(22):5.
CHENG Hua,LI Lin-ling,YUAN Hong-hui,et al.Study on the Relationship of Changes of the Endogenous Hormone Content in Castanea mollissima and Bud Differentiation[J].Northern Horticulture,2013,37(20):5.
[2]李琳玲,程 华,吴聪华,等.SPE-HPLC法同时分离测定板栗花芽中四种内源激素的研究[J].北方园艺,2014,38(01):22.
LI Lin-ling,CHENG Hua,WU Cong-hua,et al.Simultaneous Determination of 4 Endogenous Hormones in Chinese Chestnut Floral Bud by Solid Phase Extraction-High Performance Liquid Chromatography[J].Northern Horticulture,2014,38(20):22.
[3]韩素芹,郝树芹,王秀峰.观赏辣椒穴盘苗氮素营养的运转分配规律及对花芽分化的影响研究[J].北方园艺,2014,38(02):83.
HAN Su- qin,HAO Shu- qin,WANG Xiu- feng.Study on N Distribution and Its Influence on Flower Bud Differentiation of Ornamental Pepper Plug Seedling[J].Northern Horticulture,2014,38(20):83.
[4]刘国彬,兰彦平,曹均,等.板栗和锥栗远缘杂交亲和性研究[J].北方园艺,2013,37(15):1.
LIU Guo-bin,LAN Yan-ping,CAO Jun,et al.Study on the Distant Hybridization Compatibility Between Castanea mollissima and C.henryi[J].Northern Horticulture,2013,37(20):1.
[5]陈 娟,金文龙,莫亿伟.两种方法提取板栗叶片DNA的效果比较[J].北方园艺,2013,37(18):89.
CHEN Juan,JIN Wen-long,MO Yi-wei.Comparison on Genomic DNA Extracted from the Leaves of Chinese Chestnut by Two Methods[J].Northern Horticulture,2013,37(20):89.
[6]刘国彬,曹均,周连第,等.雄性不育板栗种质杂交结实特性研究[J].北方园艺,2013,37(01):4.
LIU Guo-bin,CAO Jun,ZHOU Lian-di,et al.Study on Pollinated Fruit-setting Characteristics of Male Sterility Germplasm in Chestnut[J].Northern Horticulture,2013,37(20):4.
[7]李艳萍,张义勇,李世,等.绿色食品山地板栗生产技术规程[J].北方园艺,2013,37(07):46.
[J].Northern Horticulture,2013,37(20):46.
[8]金亚征,姚太梅,丁丽梅,等.果树花芽分化机理研究进展[J].北方园艺,2013,37(07):193.
JIN Ya-zheng,YAO Tai-mei,DING Li-mei,et al.Research Progress on the Mechanism of Flower Bud Differentiation of Fruit Trees[J].Northern Horticulture,2013,37(20):193.
[9]高秀岩,谢洪刚,李坤,等.短梢修剪对葡萄花芽分化及碳氮物质代谢的影响[J].北方园艺,2013,37(19):15.
GAO Xiu-yan,XIE Hong-gang,LI Kun,et al.Effect of Leaving Four Buds Pruning on Flower Bud Differentiation and Carbon-Nitrogen Metabolism of Grape[J].Northern Horticulture,2013,37(20):15.
[10]林桂玉,田素波,郑成淑,等.菊花花芽分化期生物发光与核酸代谢的变化[J].北方园艺,2013,37(20):64.
LIN Gui-yu,TIAN Su-bo,ZHENG Cheng-shu,et al.Changes of Bioluminescence and Nucleic Acid Metabolism in Chrysanthemum During Floral Differentiation[J].Northern Horticulture,2013,37(20):64.
备注/Memo
第一作者简介:程华(1980-),男,湖北麻城人,博士,讲师,现主要从事经济林种质资源评价与利用等研究工作。E-mail:chenghua1437@126.com.