WANG Li-na,YIN Kui-de,JIN Xun,et al.Effect of Low Temperture Stress on Inclusions Change and Cell Ultrastructure of Jerusalem artichoke Tuber[J].Northern Horticulture,2013,37(09):19-22.
低温锻炼对不同菊芋品种块茎内含物和细胞超微结构的影响
- Title:
- Effect of Low Temperture Stress on Inclusions Change and Cell Ultrastructure of Jerusalem artichoke Tuber
- 文章编号:
- 1001-0009(2013)09-0019-04
- Keywords:
- Jerusalem artichoke; physiological index; ultrastructure; cold stress
- 分类号:
- S 632.9
- 文献标志码:
- A
- 摘要:
- 以“青芋1号”和“神芋”菊芋品种为试材，通过自然状态的低温锻炼，测定了2个菊芋品种块茎中总糖、还原糖、可溶性糖及淀粉含量的变化；并利用扫描透射电镜观察了低温锻炼下的块茎细胞超微结构。结果表明：抗寒性品种“青芋1号”随温度降低，总糖含量升高，还原糖含量升高，可溶性糖含量增加，淀粉含量降低；反之，非抗寒性品种“神芋”总糖含量降低，还原糖升高幅度小，可溶性糖含量降低，淀粉含量降低幅度大。细胞超微结构发现，抗寒性品种“青芋1号”在-20℃时才表现出液泡破碎，线粒体数量增多，变形及解体；非抗寒性品种“神芋”在5℃时就出现类似现象，这与植物在遭受寒冷环境条件细胞适应性变化相一致。
- Abstract:
- Using ‘Qingyu No.1’ and ‘Shengyu’ as materials,the content of total sugar，reducing sugar，soluble sugar and starch of two varieties Jerusalem artichoke tuber under the nature low temperture stress were determined.Meanwhile，tuber cells ultrastructure under cold stress by scanning transmission electron microscopy were also observed.The results showed that ‘Qingyu No.1’ had total sugar increasing，reducing sugar increasing，soluble sugar increasing，starch reducing trend.Otherwise，‘Shenyu’ had total sugar reducing，reducing sugar small increasing，soluble sugar reducing，starch more reducing trend by statistical analysis.The results of cells ultrastructure observation showed that cold resistance tuber ‘Qingyu No.1’ was vacuole crushing，the number of mitochondria increasing，deformation，disintegration under -20℃,but showed by non-cold resistance tuber ‘Shenyu’ under 5℃,this order was identical with plants cytop cell defense phenomenon cope with cold environment condition.
参考文献/References:
[1]刘祖祺,张石诚.植物抗性生理学[M].北京:农业出版社，1994:13-15. [2]何若韬,王光洁.植物寒冷驯化机理[J].植物生理生化进展,1987(5):17-29. [3]Li P H,Chen H H.Plant cold hardiness and freezing stresses[M].New York:Academic Press,1982:5-22. [4]潘红丽,王殿奎.能源植物菊芋的品种鉴定和筛选[J].黑龙江农业科学,2008(4):79-80. [5]王学奎.植物生理生化实验原理和技术[M].北京:高等教育出版社,2006:174-176. [6]李雯,邵远志,陈维信.淀粉酶活性测定方法的改进[J].植物生理学通讯,2005,41(5):655-656. [7]张景云,白雅梅,于萌,等.盐胁迫对二倍体马铃薯叶超微结构的影响[J].东北农业大学学报,2010,41(8):7-10. [8]简令成,孙龙华.抗冻植物避免细胞内结冰机制的探讨-液泡内吞作用和水外排渠道的发现.植物学集刊[M].北京:科学出版社,1983:107-113. [9]周蕴薇.翠南报春叶片细胞超微结构对低温的适应性变化[J].园艺学报,2006,33(6)：1361-1364. [10]纪忠雄.柑橘抗寒性的生理生化指标[J].园艺学报,1983,10(4):239-244. [11]扬阿明,沈征言.低温锻炼提高黄瓜幼苗耐寒性效应[J].园艺学报,1992,19(1):61-66. [12]姜卫兵,王业遴,马凯.渗透保护物质在无花果抗寒性发育中的作用[J].园艺学报,1992,19(4):371-372. [13]朱月林,曹寿椿.不结球白菜抗冻性鉴定及其相关性状的研究[J].园艺学报,1989,16(2):127-133. [14]刘祖祺,张石城.植物抗性生理学[M].北京:中国农业出版社,1994. [15]周蕴薇.低温锻炼和低温胁迫期间翠南报春叶肉细胞超微结构的适应变化[J].植物生理学通讯,2007,43(2):255-258. （该文作者还有宋群雁，工作单位为黑龙江八一农垦大学。）
相似文献/References:
[1]高畅,张鹏,朱宏,等.转il-4基因番茄生理指标的测定[J].北方园艺,2013,37(04):9.
[2]刘思妹,朱毅,郝睿,等.镉胁迫对萝卜幼苗生长及相关生理指标的影响[J].北方园艺,2014,38(04):13.
LIU Si-mei,ZHU Yi,HAO Rui,et al.Effect of Cd Stress on Growth and Related Physiological Indicators of Radish Seedling[J].Northern Horticulture,2014,38(09):13.
[3]王铁良,陈思,毛丽珍.渗灌条件下土壤水分对温室秋冬茬茄子生长效应[J].北方园艺,2014,38(04):45.
WANG Tie-liang,CHEN Si,MAO Li-zhen.Effect of Soil Moisture on Growth of Greenhouse Eggplant With[J].Northern Horticulture,2014,38(09):45.
[4]王宁.植物抗寒生理性研究进展[J].北方园艺,2014,38(04):174.
WANG Ning.Research Progress on Cold Resistance Physiology of Plant[J].Northern Horticulture,2014,38(09):174.
[5]吴春燕,宋廷宇,张晓明,等.不同灌水下限对油菜生理指标的影响[J].北方园艺,2014,38(02):37.
WU Chun- yan,SONG Ting- yu,ZHANG Xiao- ming,et al.Effects of Different Irrigation Lower Limits on Physiological Indexes of Rape[J].Northern Horticulture,2014,38(09):37.
[6]李晓文,李海梅,董 琳,等.五种草坪草蒸散特性研究[J].北方园艺,2014,38(02):71.
LI Xiao- wen,LI Hai- mei,DONG Lin,et al.Study on Evapotranspiration Characteristics of Five Kinds of Turfgrass[J].Northern Horticulture,2014,38(09):71.
[7]艾星梅,杨 越,徐永艳,等.千层金幼苗部分生理指标对水分胁迫的响应[J].北方园艺,2014,38(02):75.
AI Xing- mei,YANG Yue,XU Yong- yan,et al.Dynamic Responses of Part Physiological Indices Under Water Stress in Melaleuca bracteata Seedling Stage[J].Northern Horticulture,2014,38(09):75.
[8]王正义,王玉平.灌溉定额对酿酒葡萄生长和品质的影响[J].北方园艺,2014,38(06):39.
WANG Zheng-yi,WANG Yu-ping.Effect of Irrigation Quota on Growth and Quality of Wine Grape[J].Northern Horticulture,2014,38(09):39.
[9]吴丽君,张俊凤.高温胁迫对番茄幼苗生长和生理指标的影响[J].北方园艺,2013,37(23):19.
WU Li-jun,ZHANG Jun-feng.Effect of High Temperature Stress on Growth and Physiological Indexes of Tomato Seedlings[J].Northern Horticulture,2013,37(09):19.
[10]薛志忠,杨雅华,李可晔,等.菊芋耐盐碱性研究进展[J].北方园艺,2014,38(09):196.
XUE Zhi-zhong,YANG Ya-hua,LI Ke-ye,et al.Research Progress on Saline-Alkali Tolerance of Helianthus tuberosus L.[J].Northern Horticulture,2014,38(09):196.
备注/Memo
第一作者简介：王丽娜（1983-），女，满族，在读硕士，助理研究员，现主要从事生物化学与分子生物学等研究工作。E-mail:dqnkywln@163.com.