[1]蒋 荡,侯大斌,邓文伟,等.不同产地附子中附子灵和宋果灵含量的比较[J].北方园艺,2013,37(06):159-161.
　JIANG Dang,HOU Da-bin,DENG Wen-wei,et al.Study on Content Comparison of Fuziline and Songorine from Daughter Roots of ?Aconitum carmichaeli Produced from Different Areas[J].Northern Horticulture,2013,37(06):159-161.
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不同产地附子中附子灵和宋果灵含量的比较

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第37卷 期数: 2013年06期 页码: 159-161 栏目: 出版日期: 2013-03-30
Title:
Study on Content Comparison of Fuziline and Songorine from Daughter Roots of ?Aconitum carmichaeli Produced from Different Areas
文章编号:
1001-0009(2013)06-0159-03
作者:
蒋 荡1; 侯大斌1; 邓文伟1; 刘向鸿2; 赵祥升3
1.西南科技大学 生命科学与工程学院，四川 绵阳 621010；
2.甘肃省庆阳市林木种苗管理站，甘肃 庆阳 745000；
3.中国医科学院 药用植物研究所 海南分所，海南 万宁 571533
Author(s):
JIANG Dang1; HOU Da-bin1; DENG Wen-wei1; LIU Xiang-hong2; ZHAO Xiang-sheng3
1.College of Life Science，Southwest University of Science and Technology，Mianyang，Sichuan 621010；
2.Forest Seedling Management Station of Qingyang in Gansu Province，Qingyang，Gansu 745000；
3.Hainan Branch，Medical College of Chinese Medicinal Plant Research Institute,Wanning，Hainan 571533
关键词:
高效液相色谱-质谱法; 乌头; 附子灵; 宋果灵; 含量测定
Keywords:
HPLC-MS; Aconitum carmichaeli Debx; fuziline; songorine; quantitative determination
分类号:
S 567.9
文献标志码:
A
摘要:
以不同产地的附子为试材，采用高效液相色谱-质谱法（HPLC-MS）对不同产地附子中附子灵和宋果灵的含量进行了比较研究。结果表明：附子灵和宋果灵2种生物碱在0.116～1.16 μg/mL、0.25～2.50 μg/mL范围内标准曲线呈良好线性关系，相关系数分别为0.9998、0.9992，平均加样回收率为99.1%、99.7%；不同产地附子中附子灵和宋果灵的含量存在较大差异,四川江油的含量最高，陕西城固的最低。该方法简便快速，结果准确，重复性好，灵敏度高，可用于附子灵和宋果灵含量的同时测定，并为附子质量控制研究提供了更加合理可靠的检测方法。
Abstract:
Taking daughter roots of Aconitum carmichaeli produced from different areas as materials，the content of fuziline and songorine were analyzed using HPLC-MS method.The results showed that there had a strong linear correlation between the peak and the concentration of the fuziline and songorine within test ranges，with correlation coefficients 0.9998，0.9992 and average recovery rates of 99.1%，99.7% respectively.The content of fuziline and songorine was different in daughter roots of Aconitum carmichaeli produced from different areas,among which Jiangyou of Sichuan was the highest,Chenggu of Shaanxi was the lowest.The developed method was sensitive，accurate and suitable for the quality control of daughter roots of Aconitum carmichaeli.

参考文献/References:

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备注/Memo

第一作者简介：蒋荡（1986-），男，在读硕士，研究方向为植物分子生物学及中药质量标准研究。

责任作者：侯大斌（1965-），男，博士，教授，硕士生导师，现主要从事植物分子生物学及中草药质量标准等研究工作。E-mail：dbhou@126.com.
基金项目：国家“十一五”科技支撑计划重大专项资助项目（2007BAD89B15）。

更新日期/Last Update: 2014-08-24