[1]吴桂容,曲芬霞,余炳锋.贺州荸荠开放式组织培养体系建立[J].北方园艺,2013,37(06):110-112.
　WU Gui-rong,QU Fen-xia,YU Bing-feng.Establishment of Eleocharistuberosa System of Open Tissue Culture in Hezhou[J].Northern Horticulture,2013,37(06):110-112.

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贺州荸荠开放式组织培养体系建立

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第37卷期数: 2013年06期页码: 110-112 栏目: 出版日期: 2013-03-30

Title:: Establishment of Eleocharistuberosa System of Open Tissue Culture in Hezhou

文章编号:: 1001-0009（2013）06-0110-03

作者:: 吴桂容; 曲芬霞; 余炳锋; 贺州学院桂东特色资源开发与利用重点实验室，广西贺州 542800

Author(s):: WU Gui-rong; QU Fen-xia; YU Bing-feng; Province Key Construction Laboratory of Characteristic Resources Research and Development of Guangxi，Hezhou，Guangxi 542800

关键词:: 荸荠; 开放式组织培养; 污染率

Keywords:: Eleocharis tuberose; open tissue culture; pollution rate

分类号:: S 632.9

文献标志码:: A

摘要:: 以 “贺蹄一号”荸荠为外植体，研究了抵制剂种类和浓度、蔗糖浓度、有机成分和培养方式对贺州荸荠开放式组织培养的影响。结果表明：当次氯酸钠浓度为1.0%时，污染率最低，为23.3%，平均诱导率高达79.0%；蔗糖浓度为2%时，抑菌效果较好，污染率为23.0%；有机成分去除甘氨酸时污染率最低，为11.0%，诱导率达80.7%；接种材料在培养室中培养，污染率低但诱导率高，分别为14.0%和87.7%；但是改善玻璃温室的环境条件，从而降低污染率，是开放式组织培养的有效途径之一。

Abstract:: The ‘Heti No.1’of Eleocharis tuberosa in Hezhou was used as explants，the effects of resist agent type and concentration，the concentration of sucrose，organic ingredients and effects of culture ways in Hezhou open tissue culture were studied.The results showed that hypochlorite sodium concentration for 1.0%，pollution rate was minimum，for 23.3%，average induced rate up to 79.0%；sucrose concentration for 2.0%，the effect was better，pollution rate for 23.0%；organic components removal ammonia acid was pollution minimum，for 11.0%，induced rate up 80.7%；in vaccination material in training in the culturing room，pollution rate were low and induced rate high，respectively for 14.0% and 87.7%，but if glass greenhouse of environment conditions was improved，thus reduced pollution rate，was one of the effective ways to open tissue culture.

参考文献/References:

[1] 潘丽 . 荸荠秆枯病病原学、侵染来源及品种抗性评价研究 [D]. 武汉 : 华中农业大学 ,2012:1-2. 

[2]林辉峰,周辉明,周建金,等.荸荠的组织培养和快速繁殖探讨[J].浙江农业科学,2011(6):38-40.

[3]吴桂容,李忠芳,邓年芳,等.马蹄组织培养中的外植体灭菌及培养基筛选研究[J].贺州学院学报,2007(2):126-127.

[4]解辉,莫廷辉,曾丽星.次氯酸钠在香蕉开放式培养中应用研究[J].热带作物学报,2011,32(5):886-890.

[5]赵青年,陈永波,腾建勋,等.开放式组织培养下魔芋快繁技术[J].现代农业科技,2011(13):114-115.

[6]崔刚,单文修,秦旭,等.葡萄开放式组织培养外植体系建立[J].农业生物技术科学,2011,20(6):36-38.

备注/Memo

第一作者简介：吴桂容（1970-），女，在读博士，副教授，研究方向为植物繁育技术。E-mail：hzwgr510@163.com.

基金项目：广西技术研究与开发资助项目（桂科转10191001-6）；广西教育厅计划资助项目（201012MS209）；贺州市科学研究与技术开发计划资助项目（贺科转1012007N）；贺州学院植物学重点建设学科资助项目（2011ZDJSXK05）。

更新日期/Last Update: 2014-08-23