LIU Ping,HU Guang-yu,DING Yi-feng,et al.Effects of Maleic Hydrazide on Flowering and Florescence Physiological Indices in Chrysanthemum[J].Northern Horticulture,2013,37(06):65-69.
马来酰肼喷施对菊花开花及花期生理的影响
- Title:
- Effects of Maleic Hydrazide on Flowering and Florescence Physiological Indices in Chrysanthemum
- 文章编号:
- 1001-0009（2013）06-0065-05
- Keywords:
- MH; chrysanthemum; flower diameter; plant height; florescence; physiological indices
- 分类号:
- S 682.1+1
- 文献标志码:
- A
- 摘要:
- 以菊花“兼六香菊”品种为试材，研究了定头至花芽分化期喷施10、15、20 mg/L 3种浓度的马来酰肼（MH）水溶液对“兼六香菊花期”生理生化动态及花径、株高和花期的影响。结果表明：各处理花瓣中的可溶性蛋白与可溶性糖含量都有一定程度的下降，超氧化物歧化酶（SOD）与过氧化物酶（POD）活力均受到不同程度的抑制，而丙二醛（MDA）含量、超氧阴离子（O?2）产生速率以及花瓣浸出液的相对电导率等不利的生理指标降低较为明显。总体反应为负面生理指标的减低幅度大于正面生理指标。处理组花径减小，株高降低，开花推迟，花期延长。其中以MH 15 mg/L处理优化观赏价值、延长花期最为适中。
- Abstract:
- Taking chrysanthemum ‘Jianliuxiangju’ varieties as materails,three concentrations(10，15，20 mg/L) maleic hydrazide (MH) aqueous solution were sprayed from fixed-head to flower bud differentiation.Physiological and biochemical indices in petals，flower diameter and plant height were determined during florescence.The results showed that the contents of soluble protein and soluble sugar in petals of treatment groups had a decrease to a certain extent，activities of superoxide dismutase (SOD) and peroxidase (POD) were inhibited in varying degrees.Malondialdehyde (MDA) content，superoxide anion (O?2) production rate and petals extract relative conductivity in petals were obviously declined.The overall changes for negative physiological indices reduced greater than the positive ones.Flower diameter and plant height reduced，flowering delayed，florescence extended in treatment groups.The effects of MH 15 mg/L was the best in optimizing ornamental value and extend florescence.
参考文献/References:
[1]Willits D H,Bailey D A.The effect of night temperature on chrysanthemum flowering
：
heat-tolerant versus heat-sensitive cultivars[J].Scientia
Horticulturae,2000,83:325-330.
[2]冯渐超,王少勤,李令成.植物生长调节剂马来酰肼[J].黎明化工,1994,37(1):33-35. [3]潘瑞炽.植物生理学[M].5版.北京:高等教育出版社,2004:201. [4]王玉元.马来酰肼(MH)的使用及其毒性问题[J].植物生理学通讯,1989(6):7-13. [5]Bradford M M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein-dye binding[J].Anal Biochem,1976,72:248-254. [6]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:195-197. [7]张妙霞,孔祥生,郭秀璞,等.蒽酮法测定可溶性糖显色条件的研究[J].洛阳农专学报,1997,17(4):24-25. [8]刘萍,李明军.植物生理学实验技术[M].北京:科学出版社,2007:147-149. [9]任小林,李嘉瑞.杏果实成熟衰老过程中活性氧和几种生理指标的变化[J].植物生理学通讯,1991,27(1):34-36. [10]王爱国,罗广华.植物的超氧物自由基与羟胺的反应[J].植物生理学通讯,1990(6):55-57. [11]He B,Ye H B,Yang X E.Effects of Pb on chlorophyll contents and antioxidant enzyme activity in leaf for Pb accumulating and non-accumulating ecotypes of?Sedum alfredii(Hance) [J].J Agro-Environ Sci,2003,22:274-278. [12]候福林.植物生理学实验教程[M].北京:科学出版社,2004:90. [13]Hasegawa P M,Bressan R A,Zhu J K,et al.Plant cellular and molecular responses to high salinity[J].Annu Rev Plant Physiol Plant Mol Biol,2000,51:463-499. [14]蔡马,郭颖,韩蕊莲,等.干旱胁迫对黄土高原4种乡土禾草保护酶活性及膜系统的影响[J].西北农业学报,2012,21(1):98-103,145. [15]Dhindsa R S,Plubme D H,Indsa P L,et al.Leaf senescence and lipid peroxidation:Effect of some phythohormones and scavengers of free radicals and singlet oxygen[J].Physiol Plant,1982,56:453-457. [16]Yoshidak,Kaothienp,Matsuit,et al.Molecular biology and application of plant peroxidase genes[J].Applied Microbiology and Biotechnology,2003,60(6):665-670. [17]Alscher R G,Donahue J L,Cramer C L.Reactive oxygen species and antioxidant:relationship in green cells[J].Physiol Plant,1997,100:224-233. [18]Noctor G,Foyer C H.Ascorbate and glutathione：keeping active oxygen under control[J].Annu Rev Plant Physiol Plant Mol Biol,1998,49:249-279. [19]王宝驹,李鲁华,张伟,等.咸水灌溉对4种树木扦插苗存活率,细胞膜相对透性及丙二醛质量摩尔浓度的影响[J].西北农业学报,2012,21(2):157-161. [20]Geret F,Serafim A,Bebianno M J.Antioxidant enzyme activities，metallothioneins and liqid peroxidation as biomarkers in?Ruditapes decussates[J]. Ecotoxicology,2003,12(3):426-427. [21]Suttle J C,Kende H.Ethylene action and loss of membrane integrity during petal senescence in Tradescantia[J].Plant Physiol,1980,5,1067-1072. [22]Carlos G B,Marcela S,Juan J G,et al.Antioxidant enzymes and lipid peroxidation during aging of chrysanthemum morifolium RAM petals[J].Plant Science,1995,104:161-168. [23]刘海英,崔长海,李慧,等.马来酰肼对菊花花期叶片几个与衰老有关的生理指标的影响[J].安徽农业科学,2008,36(26):11214-11215,11235. （该文作者还有李安琪、刘骨挺，单位同第一作者。）
相似文献/References:
[1]马丽.几种试剂组合对菊花切花保鲜效果的影响[J].北方园艺,2013,37(04):135.
[2]勾畅,武慧,将达,等.激素配比对不同基因型菊花再生体系的调控[J].北方园艺,2013,37(13):135.
GOU Chang,WU Hui,JIANG Da,et al.Effects of Different Hormone Combinations on the Regeneration of ?Four Different Chrysanthemum Genotypes[J].Northern Horticulture,2013,37(06):135.
[3]妙晓莉,曹轩峰,陈建宏.菊花新品种“胭脂露”的选育[J].北方园艺,2013,37(15):172.
MIAO Xiao-li,CAO Xuan-feng,CHEN Jian-hong.Breeding of a New Chrysanthemum Variety ‘Yanzhilu’[J].Northern Horticulture,2013,37(06):172.
[4]杨红超,马丽,吴有花.6-BA与2，4-D混合保鲜剂对菊花切花保鲜效果研究[J].北方园艺,2013,37(01):166.
YANG Hong-chao,MA Li,WU You-hua.Effects of 6-BA and 2,4-D-mixed Preservative on Cut Dendranthema morifolium[J].Northern Horticulture,2013,37(06):166.
[5]尹冬梅,黄宁宇,沈 晴.春季观赏菊在上海园林中的应用调查研究[J].北方园艺,2013,37(06):76.
YIN Dong-mei,HUANG Ning-yu,SHEN Qing.Investigation of the Application of Spring Chrysanthemum in Shanghai Landscape[J].Northern Horticulture,2013,37(06):76.
[6]李翠香,胡元森,姜孝先,等.菊花愈伤组织诱导的影响因素研究[J].北方园艺,2013,37(09):132.
LI Cui-xiang,HU Yuan-sen,JIANG Xiao-xian,et al.Study on the Factors Affecting the Induction of Callus of Chrysanthemum morifolium L.[J].Northern Horticulture,2013,37(06):132.
[7]邸葆,杨际双,何雪娜.不同基质对菊花‘神马’扦插生根的影响[J].北方园艺,2012,36(04):69.
DI Bao,YANG Ji-shuang,HE Xue-na.Effect of Different Media on Rooting of Chrysanthemum ‘Jinba’ Cuttings[J].Northern Horticulture,2012,36(06):69.
[8]林桂玉,田素波,郑成淑,等.菊花花芽分化期生物发光与核酸代谢的变化[J].北方园艺,2013,37(20):64.
LIN Gui-yu,TIAN Su-bo,ZHENG Cheng-shu,et al.Changes of Bioluminescence and Nucleic Acid Metabolism in Chrysanthemum During Floral Differentiation[J].Northern Horticulture,2013,37(06):64.
[9]李荣华,阎旭东,赵松山.二十个名贵菊花品种耐盐性的筛选[J].北方园艺,2012,36(08):77.
LI Rong-hua,YAN Xu-dong,ZHAO Song-shan.Screening of Salt-tolerance for 20 Kinds of Precious Chrysanthemum[J].Northern Horticulture,2012,36(06):77.
[10]党云萍,丁媛媛,刘盼盼,等.矮化菊花试管苗生根研究[J].北方园艺,2012,36(20):112.
DANG Yun-ping,DING Yuan-yuan,LIU Pan-pan,et al.Study on Rooting of Dwarf Chrysanthemum Test Tube Seedling[J].Northern Horticulture,2012,36(06):112.
备注/Memo
第一作者简介：刘萍(1958-)，女，本科，教授，现主要从事植物生理学的教学与科研工作。E-mail：liuping5812@sina.com.