TIAN He,SHI Xiao-xin,DU Guo-qiang,et al.Effect of Substrate and Nutrient Solution Supplied on Growth of Plantlets of Apple Dwarf Rootstocks in vitro [J].Northern Horticulture,2013,37(06):8-11.
基质及营养液对苹果矮砧组培苗移栽后生长的影响
- Title:
- Effect of Substrate and Nutrient Solution Supplied on Growth of Plantlets of Apple Dwarf Rootstocks in vitro
- 文章编号:
- 1001-0009(2013)06-0008-04
- Keywords:
- apple dwarf rootstock; SH series; in vitro plantlet; transplantation substrate; nutrient solution
- 分类号:
- S 661.1
- 文献标志码:
- A
- 摘要:
- 以苹果矮化砧木SH28、SH29生根组培苗为试材，研究了不同草炭土与蛭石基质配比、草炭土处理方式及3种营养液浇灌(MS、园试配方、霍格氏配方）对移栽组培苗生长的影响。结果表明：以草炭土与蛭石1∶2或1∶1配比为移栽基质，组培苗移栽成活率较高，分别达90.77%和88.24%，显著高于草炭土与蛭石2∶1配比；同时草炭土与蛭石1∶1配比处理还可有效提高幼苗生长量。草炭土经灭菌处理后，组培苗单株叶面积及单叶片面积显著增加，苗木生长更健壮。基质中添加0.1%枯草芽孢杆菌对幼苗生长量未见显著作用。MS、霍格兰、园试3种营养液浇灌40 d后，均可使移栽幼苗生长量显著大于对照，综合效果以MS营养液促苗效果最佳。
- Abstract:
- Taking apple dwarf rootstocks of SH28 and SH29?in vitro as materials，the effect of the transplantation substrate with the mix of turfy soil and vermiculite，the pre-treating ways of the turfy soil，and the watering of nutrient solutions (with formula of MS，Garden test and Hoagland) on the growth of the plantlets after transplanted were studied.The results showed that the survival rates of the plantlets were significant higher when using the mix of turfy soil and vermiculite at ratio of 1∶1 or 1∶2，and could reach 88.24% or 90.77%，respectively，compared to the using of the ratio of 2∶1.Meanwhile，the plantlets had more growth amount when transplanted in the substrate mixed with turfy soil and vermiculite at ratio of 1∶1.The turfy soil pre-treated with autoclave had an effect on the increasing of the leaf area of the plantlet and the single leaf area，and contributed to more healthy plantlets.The supplement of 0.1% Bacillus subtilis in the substrate had no significant effect on the plantlet growth.Three kinds of the nutrient solutions(MS，Hoagland，Garden test) could accelerate the plantlet growth，which could be seen 40 days after the application，and the formula of MS showed the best in general.
参考文献/References:
[1]
邵开基
,
李登科
,
张忠仁
,
等
.SH
系列苹果矮化砧木育种研究
[J].
华北农学报
,1988,3(2):86-93.
[2]邵开基,李登科,张忠仁,等.SH系苹果、K系梨矮化砧的性状特性[J].山西果树,2001,84(2):11-12. [3]陈四维,马宝焜,吕增仁,等.长富-2苹果试管苗移栽前后叶片内部解剖构造的观察[J].河北农业大学学报,1989,120(2):99-103. [4]梁美霞,戴洪义,葛红娟.组培和大田条件下苹果叶片结构和表皮特征的比较[J].果树学报,2009,26(6):781-785. [5]Michael M B，Ann R B.Stomata of apple leaves cultured in vitro[J].Plant Cell,Tissue and Organ Culture，1989(19):85-89. [6]杜振宇,马海林,马丙尧,等.葡萄组培苗适宜生长基质研究初探[J].山东林业科技,2006,162(1):3-6. [7]段春华,刘元铅,刘翠兰,等.大樱桃Gisela砧木组培苗移栽基质筛选试验[J].山东林业科技,2005,159(4):27-28. [8]曹琴,杜俊杰.欧李组培苗移栽成活影响因子的研究[J].山西农业大学学报,2009,29(3):238-242. [9]董铁,贾克礼,王发林,等.苹果试管苗移栽技术在培育脱毒自根苗中的应用[J].甘肃农业科技,1995(3):16. [10]李双林,万贵成,杨治明,等.GS营养液在葡萄试管苗移栽中的影响[J].中外葡萄与葡萄酒,2007(4):31-32. [11]郭世荣.无土栽培学[M].北京:中国农业出版社,2003:112,114. [12]马宝焜,孙建设,高仪,等.苹果试管苗移栽技术研究[J].中国果树,1991(1):4-7,15. [13]何承忠,王立苍,许昌慧,等.马缨杜鹃组培苗移栽基质的筛选研究[J].湖北农业科学,2009,48(6):1228-1230. [14]徐进,何礼远,冯洁,等.生防细菌对马铃薯青枯病的防病增产作用研究[J].植物保护,2003,29(5)：40-42. [15]王雅平,刘伊强.枯草芽孢杆菌TG26防病增产效应的研究[J].生物防治通报,1993,9(2)：63-68. [16]于海,程广有.不同营养液对三色堇无土栽培的研究[J].北方园艺,2011(18):99-101. [17]吕芬华,蒋小留,周智萍,等.基质和营养液对组培非洲菊幼苗生长的影响[J].浙江农业科学,2002(5):228-230. [18]Wagner B W，Erwin Beck E.Cytokinins in the perennial herb Urtica dioicaL.as influenced by its nitrogen status[J].Planta,1993,190:511-518. [19]Martin R J,Sutton K H,Moyle T N,et al.Effect of nitrogen fertilizer on the yield and quality of six cultivars of autumn-sown wheat[J].New Zealand Journal of Crop and Horticultural Science,1992(20):273-282. [20]徐伟忠,朱丽霞,赵根.植物生态适应性在植物水生诱导上的运用[J].分子植物育种,2006(S1):143-150.
备注/Memo
第一作者简介：田河（1985-），男，陕西蒲城人，在读硕士，现主要从事果树生物技术研究工作。E-mail：tianh1985@163.com.