抗生素对苜蓿子叶节再生的影响
- 摘要:
- 以苜蓿品种"金皇后"为试材,研究了不同激素浓度对其子叶节再生的影响,以期为建立苜蓿子叶节离体再生体系提供依据。结果表明:不加任何激素是苜蓿子叶节芽诱导的最佳培养基;在生根阶段加入1.0mg/L的IBA和10g/L的蔗糖是生根的最佳组合。潮霉素对苜蓿子叶节芽诱导具有抑制作用,当潮霉素浓度达到20mg/L时,随着时间的延长,诱导的丛生芽几乎褐化死亡,因此其适宜筛选浓度为20mg/L。头孢霉素作为抑菌剂时,在700mg/L以内对苜蓿子叶节的再生影响不大,但随着浓度的增加子叶节的死亡率增加。
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备注/Memo
基金:国家“十一五”科技支撑计划资助项目(2011BAC07B03);;国家“973”资助项目(2012CB114204);;宁夏农发办资助项目(NTKJ-2012-12)