[1]任晗堃,李丽,董银卯,等.正交实验优选赤小豆萌芽工艺研究[J].北方园艺,2013,37(24):17-20.
　REN Han-kun,LI Li,DONG Yin-mao,et al.Study on Optimization Technology of Vigna umbellata Germination by Orthogonal Test[J].Northern Horticulture,2013,37(24):17-20.
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正交实验优选赤小豆萌芽工艺研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第37卷 期数: 2013年24期 页码: 17-20 栏目: 出版日期: 2013-12-30
Title:
Study on Optimization Technology of Vigna umbellata Germination by Orthogonal Test
文章编号:
1001-0009(2013)24-0017-04
作者:
任晗堃; 李丽; 董银卯; 张小慧
北京市植物资源重点实验室，北京工商大学 食品学院，北京 100048
Author(s):
REN Han-kun; LI Li; DONG Yin-mao; ZHANG Xiao-hui
College of Food Science，Beijing Technology and Business University，Beijing Key Laboratory of Plant Resources Research and Development，Beijing 100048
关键词:
赤小豆; 萌芽工艺; 抗敏活性; 透明质酸酶抑制率; 正交实验
Keywords:
Vigna umbellata sprouts; germination process; anti-allergic activity; hyaluronidase; orthogonal array design
分类号:
S 521
文献标志码:
A
摘要:
以9个产地的赤小豆为试材，以代表抗过敏活性的透明质酸酶抑制率为指标，在产地筛选的基础上，对光照、温度、水分3个因素通过L9（33）正交实验，以期优化赤小豆萌芽工艺条件。结果表明：在无光照、温度30℃、浇灌次数3次/d的萌芽条件下，赤小豆提取物透明质酸酶抑制率为（90.67±4.82）%。该试验工艺设计合理、结果可靠，为赤小豆萌芽抗敏功效研究提供了参考依据。
Abstract:
Taking 9 origin of Vigna umbellata as materials，hyaluronidase inhibition rate that represent anti-allergic effect，based on screening of origin，the factors of sunlight，temperature and moisture were investigated by L9(33) orthogonal test，in order to optimize the technology for Vigna umbellata sprouts.The results showed that，under the conditions for Vigna umbellata germination for at 30℃ with no illumination and irrigation 3 times/day，hyaluronidase inhibition rate reached (90.67±4.82)%.The experiment method was reasonable and stable,could be provide reference for anti-allergic activity extracted from Vigna umbellata sprouts.

参考文献/References:

[1]中国科学院中国植物志编辑委员会.中国植物志[M].66卷.北京:科学出版社，1977:336-338.

[2]肖培根.新编中药志(二)[M].北京:化学工业出版社,2001:248
[3]邱明义,李小慧,石拓,等.麻黄连翘赤小豆汤血清对肥大细胞脱颗粒,组胺生成的影响[J].中药药理与临床,2003,19(5):3-4.
[4]CherngJ M，Chiang W，Chiang L C.Immunomodulatory activities of ediblebeans and related constituents from soybean[J].Food Chemistry,2007,104(2):613-618
[5]金云隆.中药抗过敏实验刍议[J].中国中医药现代远程教育,2011,9(1):220-220
[6]刘学仁,邓佑芳,吕亚新.天然化合物抗过敏作用的研究进展[J].中药新药与临床药理,2011,22(1):123-127
[7]张波,薛文通.红小豆功能特性研究进展[J].食品科学,2012,33(9):264-266.
[8]Lee K K，Choi J D.The effects of Areca catechu L extract on anti-aging[J].International Journal of Cosmetic Science,1999,21(4):285-295.
[9]李杨,董银卯,孟宏,等.7种中草药提取物抗过敏功效及刺激性研究[J].中国实验方剂学杂志,2013,19(4):191-194.
[10]KaleM，Misar A V，Dave V，et al.Anti-inflammatory activity of Dalbergialanceolaria bark ethanol extract in mice and rats[J].Journal of Ethnopharmacology,2007,112(2):300-304.
[11]陈建,刘敏,王梅,等.麻黄连轺赤小豆汤拆方抗过敏反应作用研究[J].吉林中医药,2007,27(11):55-56.
[12]颜兵,刘龙,岳小强,等.浅析仲景对赤小豆的配伍运用[J].安徽中医学院学报，2008,27(5):7-9.
[13]Yu K W，Murthy H N，Hahn E J，et al.Ginsenoside production by hairy root cultures of Panax ginseng：influence of temperature and light quality[J].Biochemical Engineering Journal,2005,23(1):53-56.
[14]Kendrick R E，Kronenberg G H M.Photomorphogenesis in Plants[M].Dordrecht：Kluwer Academic Publishers,1993.
[15]汪海峰,鞠兴荣,何广斌,等.不同海拔高度和生长季节对银杏叶中黄酮苷含量的影响[J].林产化学与工业,2002,22(4):47-50.
[16]Kandan A，Commare R R，Nandakumar R，et al.Induction of phenylpropanoid metabolism by Pseudomonas fluorescens against tomato spotted wilt virus in tomato[J].Folia Microbiologica,2002,47(2):121-129.
[17]Tomooka N，Vaughan D，Xu R Q，et al.Japanese native Vigna genetic resources[J].Japan Agricultural Research Quarterly,2001,35(1):1-10.
[18]Isemura T，Tomooka N，Kaga A，et al.Comparison of the pattern of crop domestication between two asian beans，Azuki Bean (Vigna angularis) and Rice Bean (V.umbellata)[J].Japan Agricultural Research Quarterly,2011,45(1):23-30.

备注/Memo

第一作者简介：任晗堃（1989-），男，硕士，研究方向为植物源化妆品功效添加剂的开发。E-mail：hankun89@163.com.

责任作者：董银卯（1963-），男，硕士，教授，研究方向为植物源化妆品功能成分研究与应用。E-mail：ymdong2008@163.com.
收稿日期：2013-09-09

更新日期/Last Update: 2014-12-06